TITLE:
Substitution of Deoxyinosine as Universal Base in Oligonucleotides for DNA Ligation
AUTHORS:
Zhiliang Yu
KEYWORDS:
Deoxyinosine; Substitution; Universal Base; Ligation
JOURNAL NAME:
Engineering,
Vol.5 No.10B,
December
17,
2013
ABSTRACT:
Oligonucleotides libraries have been developed for
various applications, but the library size of oligonucleotide increases
dramatically with the addition of oligonucleotide length. To assess the
possibility of shortening library size by using universal base, deoxyinosine
(dITP), the effect of single/multiple dITP substituted in oligonucleotide on
ligation was investigated. It was found that different pairs with dITP had different
ligation patterns and pairs with dITP at different locations also showed
different ligation patterns. With the departure of substitution position from
ligation site, the ligation yield increased. Single dITP substitution at
ligation site did extremely hurt the ligation efficiency, except for I:C pair.
On the other hand, single substitution at two bases or more apart from ligation
site, there is no obvious effect on ligation. Multiple dITP substitutions can
more or less affect the ligation, besides I:C pair. This research demonstrated
that dITP can be applied to reduce oligonucleotide library size after
substitution.