TITLE:
Mycelial Growth of Paecilomyces hepiali in Various Agar Media and Yield of Fruit Bodies in Rice Based Media
AUTHORS:
Alfred Chioza, Shoji Ohga
KEYWORDS:
Agar Media; Cereal Grains Agar; Mycelial Growth; Paecilomyces hepiali
JOURNAL NAME:
Advances in Microbiology,
Vol.3 No.7,
November
27,
2013
ABSTRACT: Growth ofPaecilomyces hepialiin various agar media and
yield of fruit bodies in rice based media werestudied. The best growth in
agar media was obtained at 25℃(61.86 mm colony diameter in 14 days). The initial agar media pH
rangefrom6 to 8 was found to bethemost favourable for mycelial
growth. This study found that agars made with powders of cereal grains alone do
not support good mycelial growth ofP. hepiali. Addition of peptone
improved mycelial growth significantly. The most favourable carbon sources were
Mannose, Fructose and Glucose. Organic nitrogen sources were found to bethemost
preferred. The results demonstrated that brown rice is better than polished
rice in yield of fruit bodies. Addition of peptone was found to be quite
significant in enhancing yield of fruit bodies. Peptone, as a supplement, gave
a better yield than addition of egg yolk, albumen and a mixture of the two. The
medium with40 g brown rice, 0.325 g glucose, 0.65 g sucrose, 2 g peptone
and 65 ml corn steep liquor was found to bethemost favourable and it
yielded 19.3 g of fresh fruit bodies.