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has been cited by the following article:

• TITLE: Mycelial Growth of Paecilomyces hepiali in Various Agar Media and Yield of Fruit Bodies in Rice Based Media

  AUTHORS: Alfred Chioza, Shoji Ohga

  KEYWORDS: Agar Media; Cereal Grains Agar; Mycelial Growth; Paecilomyces hepiali

  JOURNAL NAME: Advances in Microbiology, Vol.3 No.7, November 27, 2013

  ABSTRACT: Growth ofPaecilomyces hepialiin various agar media and yield of fruit bodies in rice based media werestudied. The best growth in agar media was obtained at 25℃(61.86 mm colony diameter in 14 days). The initial agar media pH rangefrom6 to 8 was found to bethemost favourable for mycelial growth. This study found that agars made with powders of cereal grains alone do not support good mycelial growth ofP. hepiali. Addition of peptone improved mycelial growth significantly. The most favourable carbon sources were Mannose, Fructose and Glucose. Organic nitrogen sources were found to bethemost preferred. The results demonstrated that brown rice is better than polished rice in yield of fruit bodies. Addition of peptone was found to be quite significant in enhancing yield of fruit bodies. Peptone, as a supplement, gave a better yield than addition of egg yolk, albumen and a mixture of the two. The medium with40 g brown rice, 0.325 g glucose, 0.65 g sucrose, 2 g peptone and 65 ml corn steep liquor was found to bethemost favourable and it yielded 19.3 g of fresh fruit bodies.