Van Remmen, H., Ikeno, Y., Hamilton, M., Pahlavani, M., Wolf, N., Thorpe, S.R., Alderson, N.L., Baynes, J.W., Epstein, C.J., Huang, T.T., Nelson, J., Strong, R. and Richardson, A. (2003) Life-long reduction in MnSOD activity results in increased DNA damage and higher incidence of cancer but does not accelerate aging. Physiological Genomics, 16, 29-37.
http://dx.doi.org/10.1152/physiolgenomics.00122.2003

has been cited by the following article:

• TITLE: Action of protoporphyrin-IX (PP-IX) in the lifespan of Drosophila melanogaster deficient in endogenous antioxidants, Sod and Cat

  AUTHORS: Emilio Pimentel, Luz M. Vidal, Martha Patricia Cruces, Mariusz Krzysztof Janczur

  KEYWORDS: Protoporphyrin IX (PP-IX); Lifespan; Sod; Cat; Reactive Oxygen Species; Longevity; Drosophila melanogaster

  JOURNAL NAME: Open Journal of Animal Sciences, Vol.3 No.4B, November 18, 2013

  ABSTRACT: Protoporphyirin-IX (PP-IX) is a precursor of the biosynthesis of the hemo group, most of the cytochromes and the chlorophylls. The PP-IX is used for medical purposes, and recently a report indicated that it exhibits a dual action since it can decrease or increase the genetic damage caused by N-nitroso-N-ethylurea (ENU) in somatic cells of Drosophila. PP-IX is known to be able to act as an anti-or pro-oxidant agent. The aim of the present research was to study the role of PP-IX on the lifespan of Drosophila melanogaster, taking into account the fact that increasing levels of ROS can accelerate the aging process. The Canton-S strain (CS) was used as well as Sod and Cat which are deficient in the endogenous enzymes, superoxide dismutase and catalase, respectively. Groups of females and males were treated separately with 5 mg/ml of PP-IX solution. The comparison of survival curves indicates that this pigment extended the lifespan of CS. In contrast, Sod strain showed that the opposite effect and had no effect in Cat strain. The fact that PP-IX reduces the mean lifespan in Sod deficient strain might suggest a pro-oxidant action of PP-IX, and consequently the cumulating of ROS as a superoxide could have a mutagenic effect as was shown recently. The results presented evidence of the dual effect of PP-IX.