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has been cited by the following article:

• TITLE: Nickase-dependent isothermal DNA amplification

  AUTHORS: Yan He, Tao Jiang

  KEYWORDS: DNA Amplification; DNA Polymerase; Isothermal; Nickase; Strand Displacement

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.4 No.4, April 19, 2013

  ABSTRACT: We developed a nicking endonuclease dependent DNA amplification (NDA), using Nt.BstNBI to catalyze single-stranded nick on double-stranded DNA, and Bst DNA polymerase to make extension while sealing the nick and displacing the downstream strand. The displaced single-stranded DNA thereby serves as template for primers hybridization and extension, resulting in exponential synthesis of target DNA under isothermal condition. Over 105 folds target DNA amplification can be achieved in 30 minutes, generating DNA product suitable for both diagnosis and DNA cloning. This NDA strategy does not require thermal cycling or prerequisite nucleotides modification, making it suitable for application in the field and at the point-of-care.