TITLE:
Impact of the residues of detergents and disinfectants used in dairy farms on the results of inhibitor tests for raw milk
AUTHORS:
Joana Šalomskienė, Irena Mačionienė, Renata Žvirdauskienė, Dovilė Jonkuvienė
KEYWORDS:
Detergents; Disinfectants; Residues; Sensitivity; Inhibitor Tests; Raw Milk
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.4 No.2A,
February
28,
2013
ABSTRACT:
The aim of the study was to determine the impact of residues
of detergents and disinfectants on the results of most commonly used inhibitor tests
for raw milk. Microbiological test (Delvotest
SP-NT) and three rapid tests (Charm 3 MRL BL/TET2, Charm ROSA MRL BL/TET and Penzym) were used in the study. Three
concentrations (recommended by the manufacturer, 10 times lower and twice higher)
of 36 detergents and disinfectants in
raw milk were investigated. All methods did not detect concentrations of detergents and disinfectants of alkaline and acid origin 10 times lower than recommended
by the manufacturer. 39% of the investigated substances of alkaline origin were
detected by Delvotest SP-NT and Penzym; Charm tests showed non-typical results only. Delvotest SP-NT did not detect substances
of acid origin; Penzym detected 50%
of these substances, Charm tests showed only non-typical results. Delvotest SP-NT and Penzym appeared to
be more sensitive to the substances used for teat hygiene and disinfection. The
scope of rapid tests (receptor or enzymatic) does not cover the detection of detergent
and disinfectant re- sidues in milk. However, according to the non-typical results
of the test, it is possible to suspect the presence of these substances in milk.
McNemar’s and Cochran’s Q tests were used for statistical analysis of the data.