Article citationsMore>>
Nakamura, T., Alder, H., Gu, Y., Prasad, R., Canaani, O., Kamada, N., Gale, R.P., Lange, B., Crist, W.M. and Nowell, P.C. (1993) Genes on chromosomes 4, 9, and 19 involved in 11q23 abnormalities in acute leukemia share sequence homology and/or common motifs. Proc. Natl. Acad. Sci. U. S. A, 90, 4631-4635.
doi.org/10.1073/pnas.90.10.4631
has been cited by the following article:
-
TITLE:
Etoposide-induced apoptosis results in chromosome breaks within the AF9 gene: Its implication in chromosome rearrangement in leukaemia
AUTHORS:
Cynthia Patricia Nicholas, Sai-Peng Sim
KEYWORDS:
Etoposide (VP-16); Apoptosis; AF9; Chromosome Breaks; Leukaemia
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.3 No.6A,
October
30,
2012
ABSTRACT: Treatment with etoposide (VP-16) has been associated with translocation of the mixed lineage leukaemia (MLL) gene seen in treatment-related acute myeloid leukaemia (t-AML). Among the different partner genes, AF9 is the most common partner gene of MLL. AF9 shares similar structural element with the MLL gene. Various mechanisms of translocation have been proposed for the MLL gene, including apoptosis, particularly the apoptotic nuclease. In the current study, we show that VP-16 induced cleavage of the AF9 gene in both leukaemic cells and cultured normal blood cell. All the breakpoints were mapped within the BCR1 of the AF9 gene. AF9 cleavages in leukaemic cells were abolished by pre-treatment with caspase inhibitor (Z-DEVD-FMK), suggesting the involvement of caspase-activated DNase (CAD). The absence of AF9 cleavage in K562 cells further supported the involvement of apoptosis. However, AF9 cleavages in cultured normal blood cell were not eliminated by caspase inhibitor. The possible role of CAD and other apoptotic nucleases/effectors that could be involved in AF9 translocation are discussed.
Related Articles:
-
Naoki Takezako, Takashi Ishii, Akihisa Nagata, Naohiro Sekiguchi, Satoshi Noto, Akiyoshi Miwa
-
Selcuk Yildirim
-
Filippus Iipinge Tshavuka, Lin Zou
-
Gmidène Abir, Wahchi Ines, Meksi Sondes, Jeddi Ramzi, Meddeb Balkis, Saad Ali, Sennana Hlima
-
Fatih M. Uckun, Sanjive Qazi