TITLE:
Influence of Insertion of the Last Sense Codon on Expression Efficiency of Green Fluorescent Protein Gene in Escherichia coli
AUTHORS:
Xiaoliang Hao, Shota Inoue, Masahide Ishikawa
KEYWORDS:
Last Sense Codon, Gene Expression, Green Fluorescent Protein, Fluorescence Intensity
JOURNAL NAME:
Journal of Materials Science and Chemical Engineering,
Vol.3 No.6,
June
11,
2015
ABSTRACT:
We studied the relationship
between insertion of the last sense codon (i.e.,
the codon preceding the stop codon) and the efficiency of gene expression. We
inserted 64 kinds of last sense codon at the 5’ end of the stop codon of the
green fluorescent protein (GFP) gene and introduced the modified GFP genes into
Escherichia coli (E. coli). Measuring the fluorescence intensity of the GFP produced
in E. coli showed that the last sense codon influenced GFP gene expression and
when CCG was inserted as the last sense codon, fluorescence intensity of E.
coli was increased to 2.09 fold. On the other hand, insertion of CUA caused
decrease of fluorescence intensity to 0.33 fold. We hope that our findings,
which may be applicable to gene engineering, will be useful for further studies
of protein expression.