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has been cited by the following article:

• TITLE: Molecular Characterization of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Gene of Field Isolate of Edwardsiella tarda

  AUTHORS: Megha K. Bedekar, Praveena K. Soman, K. V. Rajendran, Gayatri Tripathi, M. Makesh

  KEYWORDS: E. tarda, GAPDH Gene, Vaccine, Cloning

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.6 No.2, February 11, 2015

  ABSTRACT: The E. tarda bacterial culture isolated from infected fish, was confirmed by morphological and biochemical characterization. GAPDH gene of 996 bp was amplified from bacterial genomic DNA. GAPDH gene was cloned in pTZ57R/T cloning vector. The positive clone was sequenced. The sequencing result showed very homology with published sequence from pathogenic E. tarda. The sequence (nucleotide and amino acid) divergence values were very low between E. tarda isolates from India (KF142190) and China (HQ697337-38) (Figure 4 and Figure 5). However, the divergence value was high when compared with E. tarda isolates from Japan (AB198939) and this value was higher than the inter-specific divergence value (E. tarda-E. ictaluri, E. tarda-E. coli & E. tarda-Klebsiella species).