Article citationsMore>>
Kuci, S., Wessels, J.T., Buhring, H.J., Schilbach, K., Schumm, M., Seitz, G., Loffler, J., Bader, P., Schlegel, P.G., Niethammer, D. and Handgretinger, R. (2003) Identification of a Novel Class of Human Adherent CD34-Stem Cells That Give Rise to SCID-Repopulating Cells. Blood, 101, 869-876.
http://dx.doi.org/10.1182/blood-2002-03-0711
has been cited by the following article:
-
TITLE:
Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery
AUTHORS:
Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza
KEYWORDS:
Stem Cells Recovery, CD133+, Percoll, Density Gradients
JOURNAL NAME:
Stem Cell Discovery,
Vol.4 No.3,
July
3,
2014
ABSTRACT:
Circulating CD133+ stem
cells from the peripheral blood have been shown to be able to differentiate
into numerous cell lineages. However, adults have only a small number of these
circulating stem cells. The aim of the present study was to assess a new
isolation and enrichment technique for CD133+ stem cells from peripheral blood
with the use of Percoll density gradients. Our results demonstrated the presence
of two large mononuclear bands when whole blood was centrifuged with 48% and
50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major
CD133+ enrichment at the 48% Percoll concentration in one of the two bands.
Further culture of these cells resulted in the formation of multiple
colony-forming units. Our results suggest an advantage from using a simple
Percoll gradient for successful CD133+ cell recovery, which could aid in
differentiation and transplantation protocols.
Related Articles:
-
Bélgica J. Molina, Elda Giansante, Héctor J. Finol
-
Steingrimur Stefansson, David S. Chung, Jamie Yoon, Won Seok Yoo, Young Wook Park, George Kim, David Hahn, Huyen Le, Sung-Jae Chung, Stephen P. Bruttig, David H. Ho
-
Shinji Deguchi, Haruhisa Bamba
-
Bin Gao, Ping Chen, Qifeng Jiang
-
Ying Xu, Na Guo, Jinghan Guo, Dongze Wang, Qian Xu, Xiangling Li, Zhengxin Zhang, Hongbin Yang, Ruxing Wang, Xiurong Zhao, Lei Liu