Article citationsMore>>
Assmus, B., Schachinger, V., Teupe, C., Britten, M., Lehmann, R., D?bert, N., Grünwald, F., Aicher, A., Urbich, C., Martin, H., Hoelzer, D., Dimmeler, S. and Zeiher, A.M. (2002) Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction (TOPCARE-AMI). Circulation, 106, 3009-3017.
http://dx.doi.org/10.1161/01.CIR.0000043246.74879.CD
has been cited by the following article:
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TITLE:
Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery
AUTHORS:
Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza
KEYWORDS:
Stem Cells Recovery, CD133+, Percoll, Density Gradients
JOURNAL NAME:
Stem Cell Discovery,
Vol.4 No.3,
July
3,
2014
ABSTRACT:
Circulating CD133+ stem
cells from the peripheral blood have been shown to be able to differentiate
into numerous cell lineages. However, adults have only a small number of these
circulating stem cells. The aim of the present study was to assess a new
isolation and enrichment technique for CD133+ stem cells from peripheral blood
with the use of Percoll density gradients. Our results demonstrated the presence
of two large mononuclear bands when whole blood was centrifuged with 48% and
50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major
CD133+ enrichment at the 48% Percoll concentration in one of the two bands.
Further culture of these cells resulted in the formation of multiple
colony-forming units. Our results suggest an advantage from using a simple
Percoll gradient for successful CD133+ cell recovery, which could aid in
differentiation and transplantation protocols.
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