Article citationsMore>>
Jiang, Y., Jahagirdar, B.N., Reinhardt, R.L., Schwartz, R.E., Keene, C.D., Ortiz-Gonzalez, X.R., Reyes, M., Lenvik, T., Lund, T., Blackstad, M., Du, J., Aldrich, S., Lisberg, A., Low, W.C., Largaespada, D.A. and Verfaillie, C.M. (2002) Pluripotency of Mesenchymal stem Cells Derived from Adult Marrow. Nature, 418, 41-49.
http://dx.doi.org/10.1038/nature00870
has been cited by the following article:
-
TITLE:
Percoll Gradient Optimization for Blood CD133+ Stem Cell Recovery
AUTHORS:
Rosa María López-Franco, Jorge E. Moreno-Cuevas, María Teresa González-Garza
KEYWORDS:
Stem Cells Recovery, CD133+, Percoll, Density Gradients
JOURNAL NAME:
Stem Cell Discovery,
Vol.4 No.3,
July
3,
2014
ABSTRACT:
Circulating CD133+ stem
cells from the peripheral blood have been shown to be able to differentiate
into numerous cell lineages. However, adults have only a small number of these
circulating stem cells. The aim of the present study was to assess a new
isolation and enrichment technique for CD133+ stem cells from peripheral blood
with the use of Percoll density gradients. Our results demonstrated the presence
of two large mononuclear bands when whole blood was centrifuged with 48% and
50% Percoll concentrations. Flow cytometric analysis (FACS) revealed a major
CD133+ enrichment at the 48% Percoll concentration in one of the two bands.
Further culture of these cells resulted in the formation of multiple
colony-forming units. Our results suggest an advantage from using a simple
Percoll gradient for successful CD133+ cell recovery, which could aid in
differentiation and transplantation protocols.
Related Articles:
-
Maeda Mohammad, Nahi Yaseen, Ahmad Al-Joubory, Rafal Abdullah, Noah Mahmood, Aesar A. Ahmed, Ahmed Al-Shammari
-
Manabu Akahane, Tomoyuki Ueha, Takamasa Shimizu, Yusuke Inagaki, Akira Kido, Tomoaki Imamura, Kenji Kawate, Yasuhito Tanaka
-
Yong Zhao, Aiming Wang, Xiaorong Tang, Min Li, Ling Yan, Wei Shang, Meizhu Gao
-
Moataz A. Elawady, Mohammed M. Elmaghrabi, Nesrine Ebrahim, Mona A. Elawady, Dina Sabry, Ashraf Shamaa, Alyaa Ragaei
-
Lin-Lin Ma, Nan Wang, Zhen Zhou, Jun-Yun Zhang, Xue-Gang Luo, Yong Jiang, Tong-Cun Zhang