TITLE:
Inheritance of AFLP markers and genetic linkage analysis in two full-sib families of the marine shrimp Litopenaeus vannamei (Crustacea, Decapoda)
AUTHORS:
Michele Mantovani Gonçalves, Luciana Correia de Almeida Regitano, Cosme Damião Cruz, Caio Césio Salgado, Patrícia Domingues de Freitas, João Luís Rocha, Ana Karina Guerrelhas Teixeira, Pedro Manoel Galetti-Junior
KEYWORDS:
Shrimp; Litopenaeus vannamei; AFLP; Genetic Linkage Groups
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.3,
February
28,
2014
ABSTRACT:
The cultivation of the marine species of shrimp Litopenaeus vannamei has emerged as one
of the segments that best develop in the Brazilian aquaculture, representing
the agribusiness that grew the most in recent years. Regarding the economic
importance of the farming of this species
in Brazil, further studies on genetic improvement were conducted. A F2
segregating population consisting of 192 samples for each G1 and G2 families,
from crossing inbred lines was used for the studies performed in the present
work. The genetic linkage analysis was based on polymorphic markers derived
from nine AFLP (Amplified Fragment Length Polymorphism) primers. Fourteen genetic
linkage groups including 103 segregating polymorphic markers were constructed
covering350 cMfor G1 and four genetic linkage groups including 59 markers were constructed
covering300 cMfor G2. Simple marker analyses were performed among individuals evaluated
phenotypically, finding markers linked to genes that may be potentially
important and useful to assess characteristics of economic importance for
traits related to weight and the disease IMN (idiopathic muscle necrosis). The
statistical model including markers explained major proportion of phenotypic
characteristic weight in relation to disease incidence IMN.