TITLE:
A set of SCAR markers in cluster bean (Cyamopsis tetragonoloba L. Taub) genotypes
AUTHORS:
Pratibha Sharma, Vinod Kumar, K. Venkat Raman, Kapil Tiwari
KEYWORDS:
Cluster Bean; ISSR; RAPD; SCAR
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.5 No.2,
January
24,
2014
ABSTRACT:
Cluster bean, one of the
most important cash legume crop has played an increasingly important role in
wide range of industries. Owing to the significance of molecular marker studies
in numerous applications including in genetic improvement of crops, there is an
obvious need to undertake such studies in cluster bean. In
the present work, 35 genotypes of cluster bean were collected from different
states of India and analyzed using RAPD and ISSR markers. Further SCAR marker system was introduced in order to increase
the reproducibility of the polymorphism and specificity. For this polymorphic (RP-3, 1000 bp; RP-19, 1250 bp and 1100 bp) and
geographical specific bands (RP-9, 650 bp) from RAPD as well as genotype
specific band (IS-8, 550 bp) from genotype RGC-1031 (Rajasthan) from ISSR were
selected and converted into SCAR markers. The study revealed first set of
sequence-based SCAR markers in cluster bean which found more specific
information using RAPD and ISSR profiles. One genotype specific SCAR-20 for RGC-1031
(tolerant genotype against Macrophomina phaseolina), could be used to prove identity of
the genotype for improvement as well for its genetic purity assessment. Another
SCAR-8 was selected due to its specificity for cluster bean genotypes from
Rajasthan which might be important for population admixture studies.