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Conference on Environmental Pollution and Public Health (CEPPH 2010 E-BOOK)

Wuhan,China,China,9.10-9.12,2010

ISBN: 978-1-935068-16-7 Scientific Research Publishing, USA

E-Book 1502pp Pub. Date: October 2010

Category: Engineering

Price: $80

Title: UVB Leads to Abnormal Embryos and Protein Expression in Bufo bufo gargarizans
Source: Conference on Environmental Pollution and Public Health (CEPPH 2010 E-BOOK) (pp 131-136)
Author(s): Wenwen Wang, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China 250100
Jie Liu, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China 250100
Haibin Zhao, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China 250100
Zaili Luo, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China 250100
Yongxiang Shi, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Science, Shandong University, Jinan, China 250100
Abstract: Because of ozonosphere depletion, more ultraviolet B (UVB) rays reach the earth and play an important role in the development of organisms. We studied the relationship between UVB irradiation and embryonic development in Bufo bufo gargarizans. Embryos at different developmental stages were exposed to UVB and then examined. We observed a decrease in viability and a significant increase in abnormalities at the neurula stage. A variety of abnormalities occured. Two-dimensional electrophoresis (2-DE) suggested that protein expression was significantly affected in neurula by UVB. Using Imaging Master 2D Platinum 6.0, we found that 63 protein spots disappeared, while 21 protein spots were differentially expressed after UVB treatment. Analyzed with a MALDI-TOP mass spectrometer and searched in the NCBInr database, seven differentially expressed proteins were identified. Western blotting confirmed the protein identification results using mass spectrometry. Our study provides the basis for further investigation of the cellular response to UVB and elucidates the roles of photophysical factors in the development of amphibians.
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