Genetic Variability in Germplasm Accessions of Capsicum annuum L

Shrilekha Misra, Raj Kishori Lal, Mahendra Pandurang Darokar, Suman Preet Singh Khanuja

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DOI: 10.4236/ajps.2011.25074                

Abstract

Capsicum annuum is the most widely cultivated species of peppers (chilies) in the world. For culinary purposes, its fruits are used for pungency (capsaicin) and also color (capsanthin). Capsaicin is also used for medicinal purposes particularly in anti-inflammatory formulations. Genetic divergence among 38 accessions collected from diverse locations in India (28 from Uttar Pradesh, 5 from Assam, 3 from Maharashtra and 2 from Uttaranchal), was estimated from the data pooled over 3 consecutive years for 15 morphological, growth and chemotypic characters that included days to first and second flowering, fruit onset, plant height, primary, secondary and tertiary branches, leaf surface area, fruit length and diameter, fruit surface area, fresh and dry fruit weight, capsaicin and capsanthin content. Based on this characterization the plants could be grouped into 7 clusters wherein substantial diversity among accessions was indicated by the wide range of D² values (752.901 - 1918683.00). Accessions with distinct identity were marked, which are likely to be quite suitable for breeding through hybridization combining desirable traits. The accessions labeled number 38, 27, 26, 14 and 24 to high capsaicin content (%); 35, 23, 3, 16, 29 and 11 for high capsanthin content (%) and 26 and 27 for dual purpose had characteristics desirable. Above accessions could be utilized in hybridization programme for C. annuum crop improvement.

Keywords

Genetic Diversity, Capsicum Annuum, Capsaicin and Capsanthin Content, Recombination Breeding, Geographical Distribution

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	G. Pandey and V. K. Dobhal, “Multivariate Analysis in Chilli,” Journal of Spices and Aromatic Crops, Vol. 2, No. 1-2, 1993, pp. 71-74.
[2]	M. T. P. Schifino-Wittmann and L. B. Bianchetti, “Chromosome Numbers in Wild and Semidomesticated Brazilian Capsicum L (Solanaceae) Species Do x = 12 and x = 13 Represent to Evolutionary Lines?” Biological Journal of the Linnean Society, Vol. 151, No. 2, 2006, pp. 259- 269.
[3]	E. A. Moscone, M. A. Scaldaferro, M. Grabiele, N. M. Cecchini, et al., “The Evolution of Chili Peppers (Capsicum Solanaceae) a Cytogenetic Perspective. VI International Solanaceae Conference: Genomics Meets Biodiversity,” Acta Horticulturae, Vol. 745, 2007, pp. 137- 170.
[4]	C. R. Rao, “Advanced Statistical Methods in Biometric Research,” John Wiley and Sons, New York, 1952.
[5]	D. Palevitch and L. E. Craker, “Nutritional and Medicinal Importance of Red Pepper (Capsicum spp.),” Journal of Herbs, Spices and Medicinal Plants, Vol. 3, No. 2, 1995, pp. 55-83. doi:10.1300/J044v03n02_08
[6]	B. Pickersgill, “Chromosome and Evolution in Capsicum. In S. Lantiri, 1991. Lack of Karyotype Class and Skewed Chromosome Segregation in Two Backcross Progenies of Capsicum,” Journal of Genetics and Breeding, Vol. 45, 1977, pp. 51-58.
[7]	F. A. Guzmann, H. A. C. Azurdia, M. C. Duque and M. C. de Vicente, “AFLP Assessment of Genetic Diversity of Capsicum Genetic Resources in Guatemala: Home Gardens as an Option for Conservation,” Crop Science, Vol. 45, 2005, pp. 363-370.
[8]	C. P. Sudre, C. D. Cruz, R. Rodrigues, E. M. Riva, et al., “Variaveis Multicategoricas na Determinacao da Diverencia Enetica Entre Acessos de Pimento e Pimentao,” Hortic Bras, Vol. 24, No. 1, 2006, pp. 88-93. doi:10.1590/S0102-05362006000100018
[9]	A. G. Ince, M. Karaca and A. N. Onus, “Development and Utilization of Diagnostic DAMD-PCR Markers for Capsicum Accessions,” Genetic Resources and Crop Evolution, Vol. 56, No. 2, 2009, pp. 211-221. doi:10.1007/s10722-008-9356-4
[10]	P. C. Mahalanobis, “On the Generalized Distance in Statistics,” Proceedings. National Institute of Sciences (India), Vol. 2, 1936, pp. 49-55.
[11]	B. Varalakshmi and K. Haribabu, “Genetic Divergence, Heritability and Genetic Advance in Chilli,” Indian journal of Genetics, Vol. 51, No. 2, 1991, pp.174-178.
[12]	B. Pickersgill, “Relationship between Weedy and Culti- vated Forms in Some Species of Chili Peppers (Genus Capsicum),” Evolution, Vol. 25, No. 4, 1971, pp. 683-691. doi:10.2307/2406949
[13]	J. Singh, “Improvement of Chilies,” Advances in Horticulture, Vol. 5, 1993, pp. 69-86.
[14]	Embrapa, “Exportecoes Brasileiras de Hortelicas 2000-2007, ” 2009. http.//www.cnph.embrapa.br/paginas/hortalicas_em_numeros/exportecoes_brasileiras_hortalicas_2000_2007.pdf