Variations induced in human erythrocytes by ultra-low X-ray doses

Samera M. Sallam

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DOI: 10.4236/jbpc.2011.24053                

Abstract

In this work, the effect of different ultra-low doses of X-ray on human erythrocytes was investigated. Also, the effect of ascorbic acid added to erythrocyte suspension before X-rays was studied. The mean X-ray exposure level was about 10 µGy/h. Samples of erythrocytes suspension with and without ascorbic acid was exposed to X-ray doses in the range from 2.5 to 20 µGy. The obtained results showed pronounced radio-hemolysis of erythrocytes at doses starting from nearly 7.5 µGy. The effect was enhanced, for low doses, when ascorbic acid of relatively high concentration was added to erythrocyte samples. The changes may be attributed to a dose-dependent damage by oxidative stress at the level of the whole cell and to the production of reactive oxygen species, which can cause this damage. It may be concluded that X-rays, even at low levels of exposure, can induce oxidizing effect on erythrocytes. Accordingly, such results should be taken into account for workers operating on X-rays equipments.

Keywords

Erythrocytes; Ultra-low X-rays; Hemolysis; Ascorbic Acid; Electric Conductivity

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Kayan M., Naziroglu M., Celik O., Yalman K. and Koylu H. (2009) Vitamin C and E combination modulates oxidative stress induced by X-ray in blood of smoker and non smoker radiology technicians. Cell Biochemistry and Functions, 27, 424-429. doi:10.1002/cbf.1589
[2]	Huda W. (2009) Review of radiologic physics. 3rd Edition, Lippincott Williams & Wilkins, Philadelphia.
[3]	Kocer I., Taysi S., Ertekin M.V., Karslioglu I., Gepdiremen A., Sezen O. and Serifoglu K. (2007) The effect of L-carnitine in the prevention of ionizing radiation-induced cataracts: A rat model. Graefe’s Archive for Clinical and Experimental Ophthalmology, 65, 27-33.
[4]	Riley P.A. (1994) Free radicals in biology: Oxidative stress and the effects of ionizing radiation. International Journal of Radiation Biology, 65, 27-33. doi:10.1080/09553009414550041
[5]	Agrawal A. and Kale P.K. (2001) Radiation induced peroxidative damage: Mechanism and significance. Indian Journal of Experimental Biology, 39, 291-309.
[6]	Prithivirajsingh S., Story M.D., Bergh S.A., Geara F.B., Ang K.K., Ismail S.M., Stevens C.W., Buchholz T.A. and Brock W.A. (2004) Accumulation of the common mitochondrial DNA deletion induced by ionizing radiation. FEBS Letters, 30, 227-232. doi:10.1016/j.febslet.2004.06.078
[7]	Tominaga H., Kodama S., Matsuda N., Suzuki K. and Watanabe M. (2004) Involvement of reactive oxygen species (ROS) in the induction of genetic instability by radiation. Journal of Radiation Research, 45, 181-188. doi:10.1269/jrr.45.181
[8]	Maffei F., Angelini S., Forti G.C., Vidante F.S., Lodi V., Mattioli S. and Hrelia P. (2004) Spectrum of chromosomal aberrations in peripheral lymphocytes of hospital workers occupationally exposed to low doses of ionizing radiation. Mutation Research, 547, 91-99.
[9]	Hagelstrom A.H., Gorla N.B. and Larripa I.B. (1995) Chromosomal damage in workers occupationally exposed to chronic low level ionizing radiation. Toxicology Letters, 76, 113-117. doi:10.1016/0378-4274(94)03204-K
[10]	Krokosz A. (2003) The effect of hypochlorite on human erythrocytes pretreated with x-radiation. Cellular & Molecular Biology Letters, 8, 215-219.
[11]	Klucinski P., Wojcik A., Bochenek R.G., Gminski J., Mazur B., Hrycek A., Cieslik P. and Martirosian G. (2008) Erythrocyte antioxidant parameters in workers occupationally exposed to low levels of ionizing radiation. Annals of Agricultural and Environmental Medicine, 15, 9- 12.
[12]	Ibrahim I. H. (1997) Variation in electrical conductivity of human erythrocytes caused by ascorbic acid (a new method for following hemolysis). The 3rd International Conference on Engineering Mathematics and Physics, Cairo, 2, 653-656.
[13]	Ibrahim I. H. (2006) Oxidative hemolysis of erythrocytes induced by various vitamins. International Journal of Bi- ochemistry Science, 2, 100-103.
[14]	Ibrahim I. H. (1988) Akislitelnei gemoliz erythrothitov. Ph.D. Thesis, Belarussia University, Minsk.