Preparation and Characterisation of Collagen from Freshwater Fish Scales

Fengxiang Zhang, Anning Wang, Zhihua Li, Shengwen He, Lijun Shao

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DOI: 10.4236/fns.2011.28112                

Abstract

Acid-soluble collagen (ASC) and pepsin-solubilized collagen (PSC) were prepared from the waste freshwater carp fish scales. The results of SDS-PAGE showed that purified collagens were composed of at least two different chains which were in accordance with the type I collagen with α chain composition of (α₁)₂α₂. Compared with the carp fish ordinary muscle type I collagen , porcine dermis type I collagen and other seawater fish collagens, freshwater carp fish scales collagen contained relative high half-cystine (Cys-s), but lower denaturation temperature(Td) than the porcine dermis type I collagen. These collagens had evident absorption at 230 nm by UV-Vis spectra. The spectrum X-ray diffraction showed that the collagen remained single-helix and tri-helix configuration with the minimum values of the repeat spacings (d) of about 4.48 Å and 11.87 Å. Therefore, to make more effective use of limited-resources, carp fish scales can be a potential resource for the extraction of type I collagen or gelatin.

Keywords

Carp Fish Scale; ASC (Acid-Solubilized Collagens); PSC (pepsin -solubilized collagens); Type I Collagen

Conflicts of Interest

The authors declare no conflicts of interest.

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