Genetic control of protein and glucose-anabolic-enzyme syntheses by Saccharomyces cerevisiae in the fermentation of a Nigerian rice, Oryza sativa variety “Igbimo”

Bolatito Esther Boboye, Helen Adetoun Lawal

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DOI: 10.4236/abb.2011.25052                

Abstract

This work aimed at the control of the production of protein and glucose-anabolic-enzyme (GAE) by Saccharomyces cerevisiae during the fermentation of a Nigerian rice, Oryza sativa variety “Igbimo”. The yeast was mutated with ethylmethyl sulphonate. The variants and the parental yeast were separately inoculated into cooked rice and allowed to ferment at 27℃ for 7 days after which protein content and glucose-anabolic-enzyme synthesis were determined using Dinitrosalicylic acid and Biuret reagents techniques. Mutants with varying capacities to form protein and GAE were obtained. Glucose-Anabolic-Enzyme (GAE) activity ranged from 0.25 to 12.06 Units forming five groups (classes 1, 2, 3, 4 and 5) with the average activity of 0.52, 1.52, 2.28, 4.04 and 10.63 Units respectively compared with that (0.38 Unit) of the parent strain. All the mutants synthesized protein although many of them produced it at lower level while others at higher level than the wild-type. The highest (5.92 mg/mL) and lowest (0.10 mg/mL) levels protein producers are mutants 44 and 14 respectively. Mutants No. 4, 7, 22 and 78 formed total protein similar in concentrations (1.46, 1.46, 1.45 and 1.45 mg/mL) to that of the wild yeast (1.46 mg/mL). These three sets of protein concentration have ratios 4.1 (highest), 0.07 (lowest) and 1.0 (normal) to that of the parental yeast.

Keywords

Protein; Glucose-Anabolic-Enzyme; Saccharomyces Cerevisiae; Rice Fermentation

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Ali, M.A., Hasan, S.M.K., Islam, M.N. and Islam, M.N. 2008 Study on the period of acceptability of cooked rice. Journal of Bangladesh Agricultural University, 6(2), 401-408.
[2]	Imolehin, E.D. and Wada, A.C. (2000) Meeting the rice production and consumption demands of Nigeria with improved technologies. International Rice Commission Newsletters, 49, 23-41.
[3]	Longtau, S. (2003) Multi-Agency Partnerships in West African Agriculture: A Review and Description of Rice Production Systems in Jos, Nigeria. EDO-DFID. pp. 50.
[4]	Nabila, E.Y. and Abdullahi, H.E. (2003) Effect of natural fermentation on protein fractions and in vitro protein digestibility of rice. Plant Foods for Human Nutrition, 58(3), 1-8.
[5]	Eggum, B.O. (1979) The nutritional value of rice in comparison with other cereals. In: Proceedings of Workshop on Chemical Aspects of Rice Grain Quality, Los Banos, Laguna, The Philippines and IRRI. pp. 91-111.
[6]	James, C. and McCaskill, D. (1983) Rice in the American diet. Cereal Food World, 28, 667-669.
[7]	Davidson, S., Passmore, R., Brook, J.F. and Trustwell, A.S. (1999) Human nutrition and Dietetics. 7th edition. New York. Livingstone Churchhill. pp. 296.
[8]	WHO, 1985 Energy and protein requirements. Report of a Joint FAO/WHO/UNU Expert Consultation. WHO Technical Report, Geneva. Series, 724, 206.
[9]	Juliano, B.O. 1985 Polysacccharides, proteins and lipids of rice. In: Rice Chemistry and Technology. Second edition. American Association of Cereal Chemist. Saint Paul, MN, USA, pp. 59-174.
[10]	Lu, J.J. and Chang, T.T. (1980) Rice in its temporal and spatial perspective. In: Rice production and utilization. Edited by Luh, B.S. Westport, CT, USA, AVI Publishing Co. pp. 1-74.
[11]	Campbell, G. (2000) Fermented foods-a world perspective. Food Research International, 27, 253.
[12]	Anon (2010). Fermented Foods. www.sakthifoundation.org.
[13]	Batra, L.R. and Millner, P.D. (1974) Some Asian fermented foods, beverages and associated fungi. Mycologia, 66, 942-950.
[14]	Uaboi-Egbemi, P.O. (2000) Basic Microbiology. New Waves Publishers, Lagos, Nigeria.
[15]	Dizon, F.L. and Sanchez, P.C. (1984) Mass production of red mould rice (“angkak”) and stability of the Monascus pigment. Philipp. Agriculture, 67, 25-41.
[16]	Mitchell, C.R., Mitchell, P.R. and Nissenbaum, R. (1988) Nutritional Rice Milk Production. US Patent No. 4, 8, 774, 992.
[17]	Sagara, Y. (1988) The rice surplus and new technology for rice processing in Japan. Food Fert. Technology Central Asian Pacific Region Extension Bulletin, 273, 1127.
[18]	Luh, B.S. and Chang, T.T. (1991) Overview and prospects of rice production. In: Rice Production. Volume 1. Second edition, New York. pp. 1-11.
[19]	Chang, T.T. (1993) Sustaining and Expanding the “Green Revolution” in rice. In: South East Asia’s Environment Future: The Search for Sustainability. Edited by Brookfield, H. and Byron, Y. Tokyo. pp. 201-210.
[20]	Adams, M.R. and Moses, M.O. (1995) Food Microbiology. Published by Royal Society of Chemistry, Thomas Graham House, Science Park, Cambridge. pp. 377.
[21]	Boboye, B. and Terwase, D.E. (2004) Bacteria and nutritional changes associated with natural fermentation of a Nigerian indigenous rice (Oryza sativa “IGBIMO”). Oriental Journal of Chemistry, 20(2), 235-237.
[22]	Boboye, B. (2007) Chemical and sensory evaluations of composed Nigerian-Rice-based local condiments and a commercial condiment. Oriental Journal of Chemistry, 23(3), 865-868.
[23]	Newman, R.K. and Sands, D.S. (1984) Nutritive value of corn fermented with lysine excreting Lactobacillus. Nutrition Rep. International, 30, 1287.
[24]	Ko, S.D. (1985) Growth and toxin production of Pseudomonas cocovenenans, the so called “Bongkrek Bacteria”. Asian Food Journal, 1, 78.
[25]	Boboye, B. and Alabi, O.J. (2011) Effect of monoculture fermentation on the proximate composition and sensory properties of a Nigerian indigenous rice (Oryza sativa “IGBIMO”). Food and Nutrition Sciences, unpublished.
[26]	Nout, M.J.R. (1994) Fermented foods and food safety. Food Research International, 27, 291.
[27]	Prescott, L.M., Harley, J.P. and Klein, D.A. (2002) Microbiology. Fifth International Edition. Mcgraw-Hill Publishing Company, New York.
[28]	Strachan, T. and Read, A.P. (1999) Instability of Human Genome: Mutation and DNA Repair in Human Molecular Genetics. Published by John Wiley and Sons Inc.
[29]	David, T., Anthony, J.F., Jeffrey, H. and Richard, C. (2000) An Introduction to Genetic Analysis. Third Edition. Freeman, W.F. and Company, New York. pp. 127- 143.
[30]	Redraw, C. and Miller, J. (2000) Molecular Biotechnology. Journal of Molecular Biology, 117, 577-607.
[31]	Maki, H. (2002) Origins of spontaneous mutations: Specificity and directionality of base-substitution mutagenesis. Annual Review of Genetics, 36, 279-303.
[32]	Tortora, G.J., Funke, B.R. and Case, L.C. (2002) Microbiology: An Introduction. 7th edition. Pearson Education Inc., Boston, New York.
[33]	Taggart, R. and Starr, C.B. (2006) The Unity of Life: Mutated genes and their protein products. pp. 227.
[34]	Brock, T.D., Smith, D.W. and Madigan, M.T. (1984) Biology of Microorganisms. Fourth Edition. Prentice-Hall International, Inc. pp. 244, 310.
[35]	Griffiths, A.J.F., Gelbart, W.M., Miller, J.H. and Lewontin, R.C. (1990) The Molecular Basis of Mutation. In: Modern Genetic Analysis. W.H. Freeman and Company, New York.
[36]	Parkinson, J.S. (1976) Che A, Che B and Che C genes of Escherichia coli and their role in chemotaxis. Journal of Bacteriology, 126, 758-770.
[37]	Boboye, B. and Alao, A. (2008) Effect of mutation on Trehalose-catabolic-enzyme synthesized by a tropical Rhizobium species F1. Research Journal of Microbiology, 3(4), 269-275.
[38]	Gornall, A.J., Bardwill, C.S. and David, M.M. (1949) Quantitative determination of protein. Journal of Biological Chemistry, 177, 751.
[39]	Suzuki, D.T., Griffin, J.E., Miller, H.J. and Lewontin, C.R. (2001) An Introduction to Genetic Analysis. 7th Edition. Freeman Publishers, USA., pp: 101-105.