Optimization of the Extraction Technology of Chlorogenic Acid in Honeysuckle by Response Surface Method

Tingting Cui^1*, Chao Wang², Changsong Shan¹, Peng Wu^1*

¹College of Food Science and Technology, Shandong Agriculture University, Taian, China.
²College of Horticulture Science and Engineering, Shandong Agricultural University, Taian, China.

DOI: 10.4236/oalib.1100941                

Abstract

The ultrasound-assisted extraction of chlorogenic acid from honeysuckle was modeled using response surface methodology. A three-level-three-factor Box–Behnken design was employed to optimize three extraction variables, including extraction temperature (X₁), ethanol concentration (X₂), and soak time (X₃), for the achievement of high extraction yield of the chlorogenic acid. The optimized conditions are extract temperature of 80℃, ethanol concentration of 70.37%, soak time of 9.35 h. Under this optimized conditions, the experimental yield of chlorogenic acid is 1.918%, which is well matched with the predicted yield of 1.929%.

Keywords

Design-Expert Software, Lonicera Japonica, Chlorogenic Acid, Extraction Technology Optimization

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Chinese Pharmacopoeia Commission (2010) Chinese Pharmacopoeia. China Medical Science Press, Beijing, 205-206.
[2]	Lee, E.J., Kim, J.S., Kim, H.P., et al. (2010) Phenolic Constituents from the Flowers Bud of Lonicera Japonica and Their 5-li-Poxygenase Inhibitory Activities. Food Chemistry, 120, 134-139. http://dx.doi.org/10.1016/j.foodchem.2009.09.088
[3]	Bassoli, B.K., Cassolla, P., Borba-Murad, G.R., et al. (2008) Chlorogenic Acid Reduces the Plasma Glucose Peak in the Oral Glucose Tolerance Test. Effects on Hepatic Glucose Release and Glycaemia. Cell Biochemistry and Function, 26, 320-328. http://dx.doi.org/10.1002/cbf.1444
[4]	Macrcos, A.B., Ricardo, E.S., Eliane, P.O., et al. (2008) Response Surface Methodology (RSM) as a Tool for Optimization in Analytical Chemistry. Talanta, 76, 965-977. http://dx.doi.org/10.1016/j.talanta.2008.05.019
[5]	Wu, J.H, Wang, L., Gao, F., et al. (2011) Study on Enzyme-Assisted Extraction of Lentinan. Food and Fermentation Industries, 37, 201-205 (in Chinese).
[6]	Lee, S., Bae, H., Kim, N., et al. (2008) Optimization of Growth Condition of Lentinus Edodes Mycelium on Corn Processing Waste Using Response Surface Analysis. Journal of Bioscience and Bioengineering, 105, 161-163.
[7]	Huang, L., Tang, J.L., Huang, J., et al. (2012). Research Progress in Application and Chlorogenic Acid Extraction. University Laboratory Study and Work, 113, 114-117 (in Chinese).
[8]	Abraham, S.K. (1993) Protective Effects of Chlorogenic Acid, Curcuin in and a α-Carotene Against γ-Radiation Induced in Vitro Chromosomal Damage. Mutation Research, 303, 10-11.
[9]	Yong, L., Shoulian, W. and Miaochuan, L. (2013) Optimization of Ultrasonic Extraction of Phenolic Compounds from Euryale Ferox Seed Shells Using Response Surface Methodology. Industrial Crops and Products, 49, 837-848. http://dx.doi.org/10.1016/j.indcrop.2013.07.023
[10]	Pinho, C., Melo, A., Mansilha, C. and Ferreira, I.M.P.L.V.O. (2011) Optimization of Conditions for Anthocyanin Hydrolysis from Red Wine Using Response Surface Methodology (RSM). Journal of Agricultural and Food Chemistry, 59, 50-55. http://dx.doi.org/10.1021/jf103839j
[11]	Chen, W., Wang, W.P., Zhang, H.S. and Huang, Q. (2012) Optimization of Ultrasonic-Assisted Extraction of Water-Soluble Polysaccharides from Boletus edulis Mycelia Using Response Surface Methodology. Carbohydrate Polymers, 87, 614-619. http://dx.doi.org/10.1016/j.carbpol.2011.08.029
[12]	Carrera, C., Ruiz-Rodríguez, A., Palma, M. and Barroso, C.G. (2012) Ultrasound Assisted Extraction of Phenolic Compounds from Grapes. Analytica Chimica Acta, 732, 100-104. http://dx.doi.org/10.1016/j.aca.2011.11.032
[13]	Li, Q. and Fu, C. (2005) Application of Response Surface Methodology for Extraction Optimization of Germinant Pumpkin Seeds Protein. Food Chemistry, 92, 701-706. http://dx.doi.org/10.1016/j.foodchem.2004.08.042
[14]	Sheng, Z.L., Wan, P.F., Dong, C.L. and Li, Y.H. (2013) Optimization of Total Chlorogenic Content Extracted from Flos Populi Using Response Surface Methodology. Industrial Crops and Products, 43, 778-786. http://dx.doi.org/10.1016/j.indcrop.2012.08.020
[15]	Ferreira, S.L., Bruns, R.E., Ferreira, H.S., Matosa, G.D., Davida, J.M., Brandão, G.C., et al. (2007) Box-Behnken Design: An Alternative for the Optimization of Analytical Methods. Analytical chemistry Acta, 597, 179-186. http://dx.doi.org/10.1016/j.aca.2007.07.011
[16]	Spigno, G. and De Faveri, D.M. (2009) Microwave-Assisted Extraction of Tea Phenols: A Phenomenological Study. Journal of Food Engineering, 93, 210-217. http://dx.doi.org/10.1016/j.jfoodeng.2009.01.006
[17]	Iwanhas, H., Negoro, Y., Ikeda, A., Morishita, H. and Kido, R. (1986) Inhibition by Chlorogenic Acid of Haematin-Catalysed Retinoic Acid 5, 6-Epoxidation. The Biochemical Journal, 239, 641-646.
[18]	Zhang, L., Liu, J., Zhang, P., Yan, S., He, X. and Chen, F. (2011) Ionic Liquid-Based Ultrasound-Assisted Extraction of Chlorogenic Acid from Lonicera japonica Thunb. Chromatographia, 73, 129-133. http://dx.doi.org/10.1007/s10337-010-1828-y
[19]	Merabet, S., Robert, D., Weber, J.V., Bouhelassa, M. and Benkhanouche, S. (2009) Photocatalytic Degradation of Indole in UV/TiO2: Optimization and Modeling Using the Response Surface Methodology (RSM). Environmental Chemistry Letters, 7, 45-49. http://dx.doi.org/10.1007/s10311-008-0137-2
[20]	Zhu, C. and Liu, X. (2013) Optimization of Extraction Process of Crude Polysaccharides from Pomegranate Peel by Response Surface Methodology. Carbohydrate Polymers, 92, 1197-1202. http://dx.doi.org/10.1016/j.carbpol.2012.10.073
[21]	Song, D., Liang, Y. and Yang, G. (2010) Optimization on the Ultrasonic Treatment Extracting Process for Chlorogenic Acid from Lonicera japonica Thumb by Orthogonal Design. Journal of Animal and Veterinary Medicine, 2010, 37-40 (In Chinese).
[22]	Yuan, F. (2012) Orthogonal Test Design for Optimization of Ultrasonic Extraction Process of Chlorogenic Acid from Honeysuckle. Chinese Journal of Chinese Guide, 2012, 913-916 (In Chinese).