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Laboratory Handling of Didymosphenia geminata (Lyngbye) Schmidt and the Effect of Control Efforts on Viability

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DOI: 10.4236/abb.2015.68053    3,149 Downloads   3,604 Views   Citations


Didymosphenia geminata (Lyngbye) Schmidt is a type of diatom that exists in Chile as an introduced species, invading the country and its rivers. We collected samples of D. geminata from two sampling points in Chile, assessing their viability and response to control agents. Fresh D. geminata showed more than 90% of viable granular forms (containing granules in their cytoplasm); however, the dry form presents near 50% viability. By creating dry D. geminata through exposure to 38°C temperatures for 7 days, viability values of the granular form decreased to 20%. D. geminata kept at room temperature for more than 4 weeks reported values of granular forms at 50%, while samples that were refrigerated at 4°C maintained values of granular forms at 90% for 4 weeks. Previous studies suggest that high salt concentration affects the viability of D. geminata. When taking wet D. geminata samples and exposing them to a solution of 10% NaCl for 10 minutes, we observed no differences compared to the control samples, finding granular forms at 90%. When the D. geminata was exposed to a 5% soap solution, reductions of over 90% of the granular forms were observed. Our results suggest that the viability of D. geminata is associated with the granular content within their cytoplasm, and that it is possible to alter laboratory conditions for their study. These early studies are important in order to better manipulate the model in the laboratory, allowing us to obtain new evidence regarding the microalgae’s biology through in vitro studies.

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The authors declare no conflicts of interest.

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Parodi, J. , Olivares, P. , Chavez, V. and Peredo-Parada, M. (2015) Laboratory Handling of Didymosphenia geminata (Lyngbye) Schmidt and the Effect of Control Efforts on Viability. Advances in Bioscience and Biotechnology, 6, 508-516. doi: 10.4236/abb.2015.68053.


[1] Rivera, P., Basualto, S. and Cruces, F. (2013) On the Diatom Didymosphenia geminata (Lyngbye) M. Schmidt: Its Morphology and Distribution in Chile. Gayana Botanica, 70, 154-158.
[2] Kelly, M.G. (2003) Short Term Dynamics of Diatoms in an Upland Stream and Implications for Monitoring Eutrophication. Environmental Pollution, 125, 117-122.
[3] Stoermer, E.F., Kreis Jr., R.G. and Andresen, N.A. (1999) Checklist of Diatoms from the Laurentian Great Lakes. II. Journal of Great Lakes Research, 25, 515-566.
[4] Reid, B.L., Hernandez, K.L., Frangopulos, M., Bauer, G., Lorca, M., Kilroy, C. and Spaulding, S. (2012) The Invasion of the Freshwater Diatom Didymosphenia geminata in Patagonia: Prospects, Strategies, and Implications for Biosecurity of Invasive Microorganisms in Continental Waters. Conservation Letters, 5, 432-440.
[5] Beville, S.T., Kerr, G.N. and Hughey, K.F.D. (2012) Valuing Impacts of the Invasive Alga Didymosphenia geminata on Recreational Angling. Ecological Economics, 82, 1-10.
[6] Bothwell, M.L. and Kilroy, C. (2011) Phosphorus Limitation of the Freshwater Benthic Diatom Didymosphenia geminata Determined by the Frequency of Dividing Cells. Freshwater Biology, 56, 565-578.
[7] Clearwater, S.J., Jellyman, P.G., Biggs, B.J.F., Hickey, C.W., Blair, N. and Clayton, J.S. (2011) Pulse-Dose Application of Chelated Copper to a River for Didymosphenia geminata Control Effects on Macroinvertebrates and Fish. Environmental Toxicology and Chemistry, 30, 181-195.
[8] Jellyman, P.G., Clearwater, S.J., Clayton, J.S., Kilroy, C., Blair, N., Hickey, C.W. and Biggs, B.J.F. (2011) Controlling the Invasive Diatom Didymosphenia geminata: An Ecotoxicity Assessment of Four Potential Biocides. Archives of Environmental Contamination and Toxicology, 61, 115-127.
[9] Clearwater, S.J., Jellyman, P.G., Biggs, B.J., Hickey, C.W., Blair, N. and Clayton, J.S. (2010) Pulse-Dose Application of Chelated Copper to a River for Didymosphenia geminata Control: Effects on Macroinvertebrates and Fish. Environmental Toxicology and Chemistry, 30, 181-195.
[10] Kilroy, C., Larned, S.T. and Biggs, B.J.F. (2009) The Non-Indigenous Diatom Didymosphenia geminata Alters Benthic Communities in New Zealand Rivers. Freshwater Biology, 54, 1990-2002.
[11] Bergey, E.A., Cooper, J.T. and Phillips, B.C. (2010) Substrate Characteristics Affect Colonization by the Bloom-Forming Diatom Didymosphenia geminata. Aquatic Ecology, 44, 33-40.
[12] Gillis, C.A. and Chalifour, M. (2010) Changes in the Macrobenthic Community Structure Following the Introduction of the Invasive Algae Didymosphenia geminata in the Matapedia River (Québec, Canada). Hydrobiologia, 647, 63-70.
[13] Olivares, P., Orellana, P., Guerra, G., Peredo-Parada, M., Chavez, V., Ramirez, A. and Parodi, J. (2015) Water Contaminated with Didymosphenia geminata Generates Changes in Salmo salar Spermatozoa Activation Times. Aquatic Toxicology, 163, 102-108.
[14] Crippen, R.W. and Perrier, J.L. (1974) The Use of Neutral Red and Evans Blue for Live-Dead Determinations of Marine Plankton (with Comments on the Use of Rotenone for Inhibition of Grazing). Stain Technology, 49, 97-104.
[15] Jellyman, P.G., Clearwater, S.J., Clayton, J.S., Kilroy, C., Hickey, C.W., Blair, N. and Biggs, B.J.F. (2010) Rapid Screening of Multiple Compounds for Control of the Invasive Diatom Didymosphenia geminata. Journal of Aquatic Plant Management, 48, 63-71.
[16] Bothwell, M.L., Taylor, B.W. and Kilroy, C. (2014) The Didymo Story: The Role of Low Dissolved Phosphorus in the Formation of Didymosphenia geminata Blooms. Diatom Research, 29, 229-236.
[17] Kuhajek, J.M., Lemoine, M., Kilroy, C., Cary, S.C., Gerbeaux, P. and Wood, S.A. (2014) Laboratory Study of the Survival and Attachment of Didymosphenia geminata (Bacillariophyceae) in Water Sourced from Rivers throughout New Zealand. Phycologia, 53, 1-9.
[18] Agrawal, S.C. and Singh, V. (2002) Viability of Dried Filaments, Survivability and Reproduction under Water Stress, and Survivability Following Heat and UV Exposure in Lyngbya martensiana, Oscillatoria agardhii, Nostoc calcicola, Hormidium fluitans, Spirogyra sp. and Vaucheria geminata. Folia Microbiologica, 47, 61-67.
[19] Aboal, M., Marco, S., Chaves, E., Mulero, I. and Garcia-Ayala, A. (2012) Ultrastructure and Function of Stalks of the Diatom Didymosphenia geminata. Hydrobiologia, 695, 17-24.
[20] Kilroy, C. and Bothwell, M. (2011) En-vironmental Control of Stalk Length in the Bloom-Forming, Freshwater Benthic Diatom Didymosphenia geminata (Bacillariophyceae). Journal of Phycology, 47, 981-989.
[21] Root, S. and O’Reilly, C.M. (2012) Control: Increasing the Effectiveness of Decontamination Strategies and Reducing Spread. Fisheries, 37, 440-448.
[22] Domozych, D.S., Toso, M. and Snyder, A. (2010) Biofilm Dynamics of the Nuisance Diatom, Didymosphenia geminata (Bacillariophyceae). Nova Hedwigia, 136, 249-259.

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