Share This Article:

The prevalence of virulent clonal strains of mutans streptococci in vivo and co-culture succession of the strains in vitro—Virulence potential of mutans streptococci

Abstract Full-Text HTML Download Download as PDF (Size:455KB) PP. 18-24
DOI: 10.4236/ojst.2011.12004    4,331 Downloads   7,484 Views   Citations

ABSTRACT

Purpose:To examine selected putative virulent prop-erties of mutans streptococci in two groups with dif-ferent caries activity and to examine co-culture hy-bridization of the strains in vitro. Methods: A set of strains from caries-free subjects (115) and another set from caries-active subjects (165) were isolated. Each strain was characterized for three virulence determi-nants. The clinical bacteria were then cocultured by three strains exhibiting the highest levels of virulence. Isolate colonies of last filial generation bacteria were enrichment-incubated and estimated for virulence again. RAPD-PCR and MLEE analyses were processed for parental bacteria and last filial genera-tion one. Results: No difference associated with caries activity of the subjects from whom the isolates origi-nated. Virulent properties of a filial generation strains was not different in the same generation, but was very different from their parent strains. Conclu-sion: The coexist properties of virulent polyclonal strain of MS may hold in a very general conditional sense in a dental plaque ecosystem in vivo, however, one of the co-culture strains may became dominant and displa- ced the others as the result of continuous ecological succession in vitro.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Li, M. , Lai, G. and Wang, J. (2011) The prevalence of virulent clonal strains of mutans streptococci in vivo and co-culture succession of the strains in vitro—Virulence potential of mutans streptococci. Open Journal of Stomatology, 1, 18-24. doi: 10.4236/ojst.2011.12004.

References

[1] Qi, F. and Kreth, J. (2010) Characterization of anti- competitor activities produced by oral bacteria. Methods in Molecular Biology, 666, 151-166. doi:10.1007/978-1-60761-820-1_11
[2] Hamilton, I.R. (2000) Ecological basis for dental caries. In: Kuramitsu, H.K. and Ellen, R.P. Eds., Oral bacterial ecology: The molecular basis, Horizon Scientific Press, Norfolk, pp. 219-274.
[3] Mattos-Graner, R.O., Smith, D.J., King, W.F. and Mayer, M.P. (2000) Water-insoluble glucan synthesis by mutans streptococcal strains correlates with caries incidence in 12 to 30-month-old children. Journal of Dental Research, 79, 1371-1377. doi:10.1177/00220345000790060401
[4] Yamashita, Y., Bowen, W.H., Burne, R.A. and Kura- mitsu, H.K., (1993) Role of the Streptococcus mutans gtf genes in caries induction in the specific- pathogen-free rat model. Infection and Immunity, 61, 3811-3817.
[5] Kuramitsu, H.K. (1993) Virulence factors of mutans strepto-cocci: Role of molecular genetics. Critical Reviews in Oral Biology & Medicine, 4, 159-176.
[6] Song, J.H., Kim, S.K., Chang, K.W., Han, S.K., Yi, H.K. and Jeon, J.G., (2006) In vitro inhibitory effects of Polygonum cuspidatum on bacterial viability and viru- lence factors of Streptococcus mutans and Strepto- coccus sobrinus. Archives of Oral Biology, 51, 1131- 1140. doi:10.1016/j.archoralbio.2006.06.011
[7] Yang, D.Q., Liu, T.J. and Li, S. (2006) A study of genetic diversity in lactate dehydrogenase of strepto- coccus mutans from clinical isolates. Journal of Sichuan University (Medicine Science Edition), 37, 781-784.
[8] Marcelle, M.N., José, A.C.L., Jacqueline, A., Reginaldo, B.G. and Robert, A.B. (2004) Adaptive acid tolerance response of Streptococcus sobrinus. The Journal of Bacteriology, 186, 6383-6390. doi:10.1128/JB.186.19.6383-6390.2004
[9] Hamada, S. and Slade, H.D. (1980) Biology, immunology, and cariogenicity of Streptococcus mutans. Microbiological Reviews, 44, 331-384.
[10] Loesche, W.J. (1986) Role of Streptococcus mutans in human dental decay. Microbiological Reviews, 50, 353- 380.
[11] Beighton, D., Rippon, H.R. and Thomas, H.E.C. (1987) The distribution of Streptococcus mutans serotypes and dental caries in a group of 5- to 8-year-old Hampshire schoolchildren. British Dental Journal, 162, 103-106. doi:10.1038/sj.bdj.4806033
[12] Alaluusua, S., Kleemola-Kujala, E. and Nystr?m, M. (1987) Caries in the primary teeth and salivary Strep- tococcus mutans and lactobacillus levels as indi- cators of caries in permanent teeth. Pediatric Dentistry, 9, 126-130.
[13] Carlsson, P., Olsson, B. and Bratthall, D., (1985) The relationship between the bacterium Streptococcus mutans in the saliva and dental caries in children in Mozambique. Archives of Oral Biology, 30, 265-268. doi:10.1016/0003-9969(85)90043-3
[14] Matee, M.I.N., Mikx, F.H.M., de Soet, J.S., Maselle, S.Y., de Graff, J., van Palenstein and Helderman, W.H. (1993) Mutans streptococci in caries-active and caries- free infants in Tanzania. Oral Microbiology and Immu- nology, 8, 322-324. doi:10.1111/j.1399-302X.1993.tb00582.x
[15] Aas, J.A., Griffen, A.L. and Dardis, S.R., (2008) Bacteria of dental caries in primary and permanent teeth in children and young adults. Journal of Clinical Micro- biology, 46, 1407-1417. doi:10.1128/JCM.01410-07
[16] Takahashi, N. and Nyvad, B. (2008) Caries ecology revisited: Microbial dynamics and the caries process. Caries Research, 42, 409-418. doi:10.1159/000159604
[17] Nakano, K., Lapirattanakul, J., Nomura, R., Nemoto, H., Alaluusua, S., Gr?nroos, L., Vaara, M., Hamada, S., Ooshima, T. and Nakagawa, I. (2007) Streptococcus mu- tans clonal variation revealed by multilocus sequence typing. Journal of Clinical Microbiology, 45, 2616-2625. doi:10.1128/JCM.02343-06
[18] Guo, L.H., Shi, J.N., Zhang, Y., Liu, X.D., Duan, J. and Wei, S. (2006) Identification of genetic differences be- tween two clinical isolates of Streptococcus mutans by suppression subtractive hybridization. Oral Microbiol Immunol, 21, 372-380. doi:10.1111/j.1399-302X.2006.00306.x
[19] Kuramitsu, H.K. and Wang, B.Y. (2006) Virulence properties of cariogenic bacteria. BMC Oral Health, 6, S11. doi:10.1186/1472-6831-6-S1-S11
[20] Schilling, K.M. and Bowen, W.H. (1988) The activity of glucosyltransferases adsorbed onto saliva-coated hydro- xyapatite. Journal of Dental Research, 67, 2-8. doi:10.1177/00220345880670010201
[21] Pereira, M.S., Leal, N.C., Sobreira, M., Almeida, A.M.P., Siqueira-Júnior, J.P.G. and Takak, M. (2002) Typing hu- man bovine Sthaphylococcus aureus by RAPD-PCR and ribotyping-PCR. Letters in Applied Microbiology, 35, 32-36. doi:10.1046/j.1472-765X.2002.01127.x
[22] Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685. doi:10.1038/227680a0
[23] Selander, R.K., Caugant, D.A., Ochman, H., Musser, J.M., Gilmour, M.N. and Whittam, T.S. (1986) Methods of multilocus enzyme electrophoresis for bacterial po- pulation genetics and systematics. Applied and Environ- mental Microbiology, 51, 873-884.
[24] Hardin, G. (1960) The competitive exclusion principle. Science, 131, 1292-1297. doi:10.1126/science.131.3409.1292
[25] Massey, R.C., Buckling, A., R. ffrench-Constant, (2004) Interference competition and parasite virulence. Pro- ceedings of the Royal Society - Biological Sciences 22, 785-788. doi:10.1098/rspb.2004.2676
[26] Rukayadi, Y., Suwanto, A., Tjahjono, B., Harling, R., (2000) Survival and epiphytic fitness of a nonpathogenic mutant of Xanthomonas campestris pv. Glycines. Applied and Environmental Microbiology, 66, 1183- 1189. doi:10.1128/AEM.66.3.1183-1189.2000
[27] Yin, Y., Yan, L., Jiang, J. and Ma, Z. (2008) Biological control of aflatoxin contamination of crops. Journal of Zhejiang University - Science B, 9, 787-792.

  
comments powered by Disqus

Copyright © 2018 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.