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Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) Activities in Selected Tissues of Rats Fed on Processed Atlantic Horse Mackerel (Trachurus trachurus)

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DOI: 10.4236/abb.2015.63014    3,940 Downloads   4,852 Views   Citations

ABSTRACT

Trachurus trachurus (locally called kote) is a table fish that has become increasingly important in the Nigerian diet because of its low price. Therefore, this study investigated the effects of processed kote on the activities of alkaline phosphatase (ALP), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in selected tissues of weaned male albino rats using standard methods. Chemical analyses were carried out on processed fillet, skin, head & bone (SHB) diets under standard conditions. 40 male rats (40.76 ± 2.42 g) were fed with processed fillet or SHB diets for 14 days to assay for the activities of ALP in harvested samples of brain, liver, kidney, heart, stomach, small intestine and spleen; ALT and AST in the liver and heart. All data were subjected to analysis of variance by Duncan’s multiple range test and considered significant at a minimum of p < 0.05. Levels of ALP & AST in the liver, heart, kidney and brain were reduced (p < 0.05) in rats fed on test diets compared with the controls, but within accepted limit. The level of ALT in the kidney, stomach and small intestine were elevated (p < 0.05) compared to the controls, but within the accepted limit. In conclusion, coal smoked fillet and SHB greatly improved healthy growth of the rats, followed by the wood smoked and poached diets. Results suggested that processed kote SHB could be a veritable source of valuable nutrients for human food and animal feed.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Adeyemi, O. , Osilesi, O. , O. Adebawo, O. , D. Onajobi, F. , Oyedemi, S. and Afolayan, A. (2015) Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST) and Alanine Aminotransferase (ALT) Activities in Selected Tissues of Rats Fed on Processed Atlantic Horse Mackerel (Trachurus trachurus). Advances in Bioscience and Biotechnology, 6, 139-152. doi: 10.4236/abb.2015.63014.

References

[1] FOS-Federal Office of Statistics (1990) Nigerian Fish Production by Sectors. Lagos Nigerian Government Printers, Lagos, 40.
[2] Olatunde, A.A. (1998) Approaches to the Study of Fisheries Biology in Nigeria Inland Waters. Proceedings of International Conference of Two Decades of Research in Lake Kainji, 338-541.
[3] Omojowo, F.S.I., Garba, L. and Ihuahi, J.A. (2009) Comparative Assessment of Potassium Sorbate and Sodium Metabisulphite on the Safety and Shelf Life of Smoked Catfish. Nature and Science, 7, 10-17.
[4] Milo, C. and Grosch, W. (1996) Changes in the Odorants of Boiled Salmon and Cod as Affected by the Storage of The Raw Material. Journal of Agricultural and Food Chemistry, 44, 2366-2371.
http://dx.doi.org/10.1021/jf9507203
[5] Durnford, E. and Shahidi, F. (1998) Flavour of Fish Meat.
[6] Varlet, P.C. and Serot, T. (2007) Volatile Aldehyde in Smoke: Analysis Methods, Occurrence and Mechanism of Formation. Food chemistry, 105, 1536-1556.
http://dx.doi.org/10.1016/j.foodchem.2007.03.041
[7] White, J.S. (2009) Misconception about High-Fructose Corn Syrup: Is It Uniquely Responsible for Obesity, Reactive Dicarbonyl Compounds, and Advanced Glycation Endproducts? Journal of Nutrition, 139, 1219S-1227S.
http://dx.doi.org/10.3945/jn.108.097998
[8] Goldberg, T., Cai, W., Peppa, M., Dardaine, V., Baliga, B.S., Uribarri, J. and Vlassara, H. (2004) Advanced Glycooxidation Products In Commonly Consumed Foods. Journal of the American Dietetic Association, 104, 1287-1291.
http://dx.doi.org/10.1016/j.jada.2004.05.214
[9] Golgi, A., Beckman, J.A., Schmidt, A.M. and Creager, M.A. (2006) Advancd Glycation Endproducts; Sparking the Development of Diabetic Vascular Injury. Circulation, 114, 597-605.
http://dx.doi.org/10.1161/CIRCULATIONAHA.106.621854
[10] Adeyemi, O.T., Osilesi, O.O., Onajobi, F.D., Adebawo, O.O., Oyedemi, S.O. and Afolayan, A.J. (2013) Effect of Processing on the Proximate and Mineral Compositions of Trachurus trachurus: A Fish Commonly Consumed in Nigeria. Journal of Emerging Trends in Engineering and Applied Sciences (JETEAS), 4, 378-385.
[11] Ophardt, C.E. (2003) Diagnostic Serum Enzymes.
http://www.elmhurst.edu/~chm/vchembook/641serumenzymes.html
[12] Adeniyi, A.F., Adeleye, J.O. and Adeniyi, C.Y. (2010) Diabetes, Sexual Dysfunction and Therapeutic Exercise: A 20 Year Review. Current Diabetes Reviews, 6, 201-206.
[13] USDA (The Economic Research Service of the USDA) (2006) Global Food Markets: Briefing Rooms.
http://www.ers.usda.gov/Briefing/
[14] Gerrard, J.A., Lasse, M., Cottam, J., Healy, J.P., Fayle, S.E., Rasiah, I. and Brown, P.K. (2012) Aspects of Physical and Chemical Alternatives to Proteins during Food Processing—Some Implication for Nutrition. British Journal of Nutrition, 108, S288-S297.
[15] FAO/UN (2007) State of the World’s Forests 2007. Food and Agriculture Organization of the United Nations, Rome.
http://www.fao.org/docrep/009/a0773e/a0773e00.htm
[16] FAO/WHO (1991) Protein Quality Evaluation Report of Joint FAO/WHO Expert Consultation. FAO Food and Nutrition Paper, Food and Agriculture Organization of the United Nations, Rome, No (51), 247.
[17] Association of Official Analytical Chemists (AOAC) (2002) Official Me-thods of Analysis. 17th Edition, Association of Official Analytical Chemists, Washington DC.
[18] Reinik, M., Tamme, T., Roasto, M., Juhkam, K., Tenno, T. and Kiis, A. (2007) Polycyclic Aromatic Hydrocarbons (PAHs) in Meat Products and Estimated PAH Intake by Children and the General Population in Estonia. Food Additives & Contaminants, 24, 429-437.
http://dx.doi.org/10.1080/02652030601182862
[19] Association of Official Analytical Chemists—AOAC (1984) Official Methods of Analysis of the Association of Official Analytical Chemists. 14th Edition, AOAC, Arlington, 1141 p.
[20] Okalebo, J.R., Gathua, K.W. and Woomer, P.L. (2004) Laboratory Methods of Soil and Plant Analysis: A Working Manual. 2nd Edition, 128 p.
[21] Oyedemi, S.O., Bradley, G. and Afolayan, A.L. (2011) Beneficial Effect of Aqueous Stem Bark Extracts of Strychnos henninhsii Gilg in Streptozotocin-Nicotinamide Induced Type 2 Diabetic Wista Rats. International Journal of Pharmacology, 7, 774.
[22] Bassey, O.A., Lowry, O.H. and Brock, M.J. (1946) A Method for the Rapid Determination of Alkaline Phosphates with Five Cubic Millimetres of Serum. Journal of Biological Chemistry, 164, 321-325.
[23] Wright, P.J., Leatherwood, P.D. and Plummer, D.T. (1972) Enzymes in Rats: Alkaline Phosphatase. Enzymologia, 42, 317-327.
[24] IFCC (International Federation of Clinical Chemistry) (1980) Physicochemical Quantities and Units in Clinical Chemistry. Journal of Clinical Chemistry & Clinical Biochemistry, 18, 829-854.
[25] Statistical Package for Social Sciences for Windows (SPSS) 14.0 (2005) Computer Program, MS. For Windows, Version 14.00, SPSS Inc., Chicago. Release 14.0.
http://www.Spss.com
[26] Duncan, D.B. (1955) Multiple Range and Multiple F-Test. Biometrics, 11, 1-5.
[27] Food and Agricultural Organization and World Health Organization (1991) Protein Quality Evaluation: Report of a Joint FAO/WHO Expert Consultation. Food and Agricultural Organization of the United Nations, Rome, Food and Nutrition Paper 51, 26-28.
[28] Aduku, A.O. (2005) Tropical Feed Stuff Analysis Table. Department of Animal Science, Faculty of Agriculture, Ahmadu Bello University, Zaria.
[29] Delorme, C.B. and Gordon, C.L. (1983) The Effect of Pectin on the Utilization of Marginal Levels of Dietary Protein by Weanling Rats. Journal of Nutrition, 113, 2432-2441.
[30] Benevenga, N.J., Calvert, C., Eckhert, C.D., Fahey, G.C., Greger, J.L., Keen, C.L., Knapka, J.J., Magalhaes, H., Bucknell, O., Olav T., Reeves, P.G., Anderson Shaw, H., Smith, J.E. and Steele, R.D. (1995) Nutrient Requirements of Laboratory Animals. 4th Revised Edition. Subcommittee on Laboratory Animal Nutrition, Committee on Animal Nutrition, Board on Agriculture, National Research Council. National Academy of Sciences, Washington DC, 1-79.
[31] Wilber, C.G. and Gilchrist, R.D. (1965) Organ Weight: Body Weight Ratios in the Mongolian Gerbil, Meriones Unguiculatus. Chesapeake Science, 6, 109-114.
http://dx.doi.org/10.2307/1351327
[32] Bailey, S.A., Zidell, R.H. and Perry, R.W. (2004) Relationships between Organ Weight and Body/Brain Weight in the Rat: What Is the Best Analytical Endpoint? Toxicologic Pathology, 32, 448-466.
http://dx.doi.org/10.1080/01926230490465874
[33] Kakade, M.L., Simons, N.R., Liener, I.E. and Lambert, J.W. (1972) Biochemical and Nutritional Assessment of Different Varieties of Soybean. Journal of Agricultural and Food Chemistry, 20, 87-90.
http://dx.doi.org/10.1021/jf60179a024
[34] Luiz, C.J., Jose, C. and Robert, O.X. (1998) Basic History. A Large Medical Textbook. 9th Edition.
[35] Muhammad, N.O. (2007) Studies on the Nutritional and Toxicological Aspects of Terminalia catappa Seeds Fermented by Aspergillus niger. Ph.D. Thesis Submitted to Department of Biochemistry, University of Ilorin, Ilorin, 143 p.
[36] Adeyemi, O.T. and Muhammad, N.O. (2010) Effect of Aspergillus Niger Fermented Chrysophyllum Albidum Seed Meal on Growth and Haematological Parameters in Rats. International Journal of Bioscience, 5, 3.
[37] Shanhjahan, M., Sabitha, K.E., Mallika, J. and Hyamala-Devi, C.S. (2004) Effect of Solanum tribatum against Carbon Tetrachloride Induced Hepatic Damage in Albino Rats. Indian Journal of Medical Research, 120, 194-198
[38] Akanji, M.A., Olagoke, O.A. and Oloyede, O.B. (1993) Effect of Chronic Consumption of Metabisulphite on the Integrity of the Kidney Rat Cellular System. Toxicology, 81, 173-179.
http://dx.doi.org/10.1016/0300-483X(93)90010-P
[39] Rao, M.N. (2006) Medical Biochemistry: For Medical, Dental, Nursing, Physiotherapy, Pharmacy, Food Science, Nutrition and Science Students. 2nd Revised Edition, New Age International (P) Limited Publishers, New Delhi, 743-780.
www.newagepublishers.com
[40] Ruothalo, K. (2008) VCA Quality Care Pet Adoption Insurance Pet Care. VCA Antech Inc., Los Angeles.
[41] Jaroslaw, S., Armand, M., Gizowska, M., Marcinek, M., Sasim, E., Szafran, E., et al. (2009) Ceramic-in-Polymer versus Polymer-in-Ceramic Polymeric Electrolytes—A Novel Approach. Journal of Power Sources, 194, 66-72.
http://dx.doi.org/10.1016/j.jpowsour.2009.01.070
[42] Anderson, A.J. (1968) Effect of Anti-Inflammatory Drug on the Enzyme Activity of Rat Liver Granular Fraction Which Increases Vascular Permeability. Biochemical Pharmacology, 17, 2253-2264.
http://dx.doi.org/10.1016/0006-2952(68)90032-4
[43] Nelson, D.L. and Cox, M.M. (2003) Lehninger Principles of Biochemistry. 3rd Edition, Worth Publishers Inc., New York, 626, 845.
[44] Jens, J.J. and Hanne, H. (2002) A Review on Liver Function Test. The Danish Hepatitis C.
http://home3.inet.tele.dk/omni/hemochromatosis_iron.htm
[45] Ioannou, G.N., Weiss, N.S., Boyko, E.J., Mozaffarian, D. and Lee, S.P. (2006) Elevated Serum Alanine Aminotransferase Activity and Calculated Risk of Coronary Heart Disease in the United States. Hepatology, 43, 1145-1151.
http://dx.doi.org/10.1002/hep.21171

  
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