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Fourier Transform Infrared Spectroscopy Typing an Enterococcus sp.

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DOI: 10.4236/jmp.2014.516169    3,706 Downloads   4,156 Views   Citations

ABSTRACT

Enterococcus species are one of the leading causes of nosocomial infections, which are difficult to treat specially with the rise of its Vancomycin resistant. Studies of Enterococcus isolates are essential for epidemiological investigation. Typing Enterococci is often based on the traditional phenotypic as well as genotypic methods. In this study Fourier-transform infrared (FTIR) spectroscopy is used as a novel phenotypic approach to the typing of Enterococci. FTIR spectroscopy results compared to antibiotic susceptibility testing and PCR amplification of Vancomycin gene results; the analysis showed that, 6 isolates were positive for Van gene (4 of VanA, 1 of VanB and 1 VanA plus VanB). Three of VanA and VanA plus VanB were resistant to all antibiotic tested (Ampicillin, Teicoplamin and Vancomycin) and VanB was found to be sensitive. FTIR spectroscopy (first derivatives) divided the isolates into 8 groups. 3 groups of VanA (4 isolates), one of VanB (one isolate), one of VanA plus VanB (one isolate) and the other 13 Enterococcus isolates were divided into 3 clusters. The study demonstrated that FTIR spectroscopy has good discriminative capacity and high reproducibility as compared to other techniques.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Taha, M. , Eideh, H. , Saed, S. and Jaber, H. (2014) Fourier Transform Infrared Spectroscopy Typing an Enterococcus sp.. Journal of Modern Physics, 5, 1698-1707. doi: 10.4236/jmp.2014.516169.

References

[1] Bodnar, U.R., Noskin, G.A., Suriano, T., Cooper, I., Reisberg, B.E. Peterson, L.R. (1996) Journal of Clinical Microbiology, 34, 2129-2132.
[2] Buschelman, B.J., Bale, M.J. and Jones, R.N. (1993) Diagnostic Microbiology and Infectious Disease, 16, 119-122.
http://dx.doi.org/10.1016/0732-8893(93)90005-R
[3] Cetinkaya, Y., Falk, P. and Mayhall, C.G. (2000) Clinical Microbiology Reviews, 13, 686-707.
[4] Cheng, S., McCleskey, F.K., Gress, M.J., Petroziello, J.M., Liu, R., Namdari, H., Beninga, K., Salmen, A. and DelVecchio, V.G. (1997) Journal of Clinical Microbiology, 35, 1248-1250.
[5] Clark, N.C., Weigel, L.M., Patel, J.B. and Tenover, F.C. (2005) Antimicrobial Agents and Chemotherapy, 49, 470-472.
http://dx.doi.org/10.1128/AAC.49.1.470-472.2005
[6] Devriese, L.A., Pot, B., Van Damme, L., Kersters, K. and Haesebrouck, F. (1995) International Journal of Food Microbiology, 26, 187-197.
http://dx.doi.org/10.1016/0168-1605(94)00119-Q
[7] Dziuba, B., Babuchowski, A., Niklewicz, M. and Brzozowski, B. (2006) Milchwissenschaft, 61, 146-149.
[8] Goulden, J.D.S. and Sharpe, M.E. (1958) Microbiology, 19, 76-86.
http://dx.doi.org/10.1099/00221287-19-1-76
[9] Greensteet, J.E.S. and Norris, K.P. (1957) Spectrochimica Acta, 9, 177-182.
http://dx.doi.org/10.1016/0371-1951(57)80131-3
[10] Helm, D., Labischinski, H. and Naumann D. (1991) Journal of Microbiological Methods, 14, 127-142.
http://dx.doi.org/10.1016/0167-7012(91)90042-O
[11] Jackson, M. and Mantsch, H. (1996) Biomedical Infrared Spectroscopy. In: Mantsch, H.H. and Chapman, D., Eds., Infrared Spectroscopy of Biomolecules, Wiley-Liss, New York.
[12] Jones, R.N., Marshall, S.A., Pfaller, M.A., Wilke, W.W., Hollis, R.J., Erwin, M.E., Edmond, M.B. and Wenzel, R.P. (1997) Diagnostic Microbiology and Infectious Disease, 29, 95-102.
http://dx.doi.org/10.1016/S0732-8893(97)00115-6
[13] Ke, D., Picard, F.J., Martineau, F., Menard, C., Roy, P.H., Ouellette, M. and Bergeron, M.G. (1999) Journal of Clinical Microbiology, 37, 3497-3503.
[14] Kümmerle, M., Scherer, S. and Seiler, H. (1998) Applied and Environmental Microbiology, 64, 2207-2214.
[15] Maquelin, K., Kirschner, C., Choo-Smith, L.P., Ngo-Thi, N.A., van Vreeswijk, T., Stammler, M., Endtz, H.P., Bruining, H.A., Naumann, D. and Puppels, G.J. (2003) Journal of Clinical Microbiology, 41, 324-329.
http://dx.doi.org/10.1128/JCM.41.1.324-329.2003
[16] Margarita, P. and Quinteiro, R. (2000) Clinical Microbiology Newsletter, 22, 57-61.
http://dx.doi.org/10.1016/S0196-4399(00)88850-9
[17] Massai, R., Bantar, C., Lopardo, H., Vay, C. and Gutkind, G. (2007) Revista Argentina de Microbiología, 39, 199-203.
[18] Monstein, H.J., Quednau, M., Samuelsson, A., Ahrne, S., Isaksson, B. and Jonasson, J. (1998) Microbiology, 144, 1171-1179.
http://dx.doi.org/10.1099/00221287-144-5-1171
[19] Nakamoto, K. (1970) Infrared Spectra of Inorganic and Coordination Compounds. John Wiley, New York, 98.
[20] Naumann, D. (1998) Infrared and NIR Raman Spectroscopy in Medical Microbiology. In: Manch, H.H. and Jackson, M., Eds., Infrared Spectroscopy: New Tool in Medicine, SPIE, Bellingham, 245-257.
[21] Naumann, D., Helm, D. and Labischinski, H. (1991) Nature, 35, 81-82.
http://dx.doi.org/10.1038/351081a0
[22] Naumann, D., Labischinski, H. and Giesbrecht, P. (1991) The Characterization of Microorganisms by Fourier-Transform Infrared Spectroscopy (FT-IR). In: Nelson, W.H., Ed., Modern Techniques for Rapid Microbiological Analysis, VCH, New York, 43-96.
[23] Abu-Teir, M., Abu-Taha, M.I., Al-Jamal, A. and Eideh, H.K. (2008) Journal of Applied Biological Sciences, 2, 113-119.
[24] National Committee for Clinical Laboratory Standards, NCCLS (1999) Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals. Approved Standard M31-A., Wayne, 25.
[25] Oberreuter, H., Seiler, H. and Scherer, S. (2002) International Journal of Systematic and Evolutionary Microbiology, 52, 91-100.
[26] Quintiliani Jr., R., Evers, S. and Courvalin, P. (1994) FEMS Microbiology Letters, 119, 359-364.
http://dx.doi.org/10.1111/j.1574-6968.1994.tb06913.x
[27] Ralph, H.P. (1982) General Chemistry. Principals and Modern Applications. Prentice Hall, Upper Saddle River, 215-218.
[28] Riddle, J.W., Kabler, P.W., Kenner, B.A., Bordner, R.H., Rookwood, S.W. and Stevenson, H.J.R. (1956) Journal of Bacteriology, 72, 593-603.
[29] Roger, M., Faucher, M.C., Forest, P., St-Antoine, P. and Coutlee, F. (1999) Journal of Clinical Microbiology, 37, 3348-3349.
[30] Schaberg, D.R., Culver, D.H. and Gaynes, R.P. (1991) The American Journal of Medicine, 91, 72S-75S.
http://dx.doi.org/10.1016/0002-9343(91)90346-Y
[31] Schuster, K.C., Mertens, F. and Gapes, J.R. (1999) Vibrational Spectroscopy, 19, 467-477.
http://dx.doi.org/10.1016/S0924-2031(98)00058-7
[32] Vincent, S., Knight, R.G., Green, M., Sahm, D.F. and Shlaes, D.M. (1991) Journal of Clinical Microbiology, 29, 2335-2337.
[33] Cherepy, N.J., Shreve, A.P., Moore, L.J., Boxer, S.G. and Mathies, R.A. (1997) Biochemistry, 36, 8559-8566.
http://dx.doi.org/10.1021/bi970024r
[34] Alvarez-Ordónez, A. and Prieto, M. (2010) Applied and Environmental Microbiology, 76, 7598-7607.
[35] Yang, J.L., Wang, M.S., Cheng, A.N., Pan, K.C., Li, C.F. and Deng, S.X. (2008) World Journal of Gastroenterology, 14, 2872-2876.
http://dx.doi.org/10.3748/wjg.14.2872

  
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