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Molecular cloning, expression and characterization of a novel geneβ-N-acetylglucosaminidase from Bombyxmori

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DOI: 10.4236/abb.2011.23019    4,101 Downloads   9,416 Views   Citations

ABSTRACT

Previously, we have reported that a gene encoding Bombyxmoriβ-N-acetylglucosaminidase 2 (BmGlcNAcase2) has been identified differentially expressed in the midgut of Bombyxmori strain NB resistant to nucleopolyhedrovirus (BmNPV), strain 306 susceptible to NPV and a near isogenic line BC9 with similar genetic background to 306 but resistant to NPV by two-dimensional gel electrophoresis (2-DE). To get more knowledge about the relationship between β-N-acetylglucosaminidase and the resistance of NPV, in this study, the 1542 bp open reading frame of a putative bombyxmoriβ-N-acetylglucosaminidase 2 gene (BmGlcNAcase2) was amplified from a pool of bombyxmoricDNAs and inserted into the prokaryotic expression plasmid pET-30a(+).Western blotting analysis showed that BmGlcNAcase2 was expressed in hemolymph, ovary, testis, fat body, trachea, midgut and silk gland of fifth instar larvae respectively . Immunofluoresence analysis indicated that BmGlcNAcase2 was mainly located to the cytoplasm or some structure in cytoplasm.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Chang, C. , Liu, X. and Chen, K. (2011) Molecular cloning, expression and characterization of a novel geneβ-N-acetylglucosaminidase from Bombyxmori. Advances in Bioscience and Biotechnology, 2, 123-127. doi: 10.4236/abb.2011.23019.

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