Studying Molecular Aspects of the Blood-Brain Barrier Using an in Vitro Model: Contribution of a Global Proteomics Strategy ()
1. Introduction
The number of patients on haemodialysis in Saudi Arabia is increasing with approximately 7020 patients currently receiving therapy [1] [2] . These patients are at higher risk of acquiring post-transfusion HCV infection. Anti-HCV prevalence rates among haemodialysis patients in Europe and Australia vary from 5.5% to 20%, whilst in Japan and Taiwan the rates are between 18% to 47% [3] - [5] .
Studies conducted in the 1990s using first-generation EIA tests revealed a prevalence of antibodies to hepatitis C virus (anti-HCV) among Saudi haemodialysis patients of 30.7% to 48.7% [6] - [10] . However, later studies that used second-generation EIA and confirmatory tests, found that the prevalence of anti-HCV ranged from 52.5% to 72.3% [11] - [15] . However, using HCV RNA assay, the prevalence rate reported by some centers reached as high as 94.7%. Furthermore, Al Meshari et al. [16] reported a prevalence rate of anti-HCV of 88.4% among 43 haemodialysis patients. However, the same authors reported a prevalence rate of anti-HCV of 76% in the same group using third-generation recombinant immunoblot assay (RIBA 3.0).
A more recent study by Qadi et al. [2] on 81 Bahraini and 34 Saudi haemodialysis patients revealed a prevalence rate of HCV infection of 9.24% and 0.30%, respectively. The most prevalent HCV genotypes in the Bahraini patients were HCV 1a/1b and HCV 4, while the prevalent genotypes in the Saudi patients were HCV 2/2a and HCV 4.
Although blood transfusion is the major cause of HCV infection in haemdialysis patients, a study by Alfurayh et al. [17] showed that nosocomial transmission of HCV in haemodialysis units also plays a significant role. In their study, HCV RNA was found on the hands of some personnel working with dialysis patients, in spite of adherence to standard precautions.
Currently, there is limited data published on the relationship between HLA types and HCV infection in the Saudi population. The aim of this study was to determine the relationship between the HLA types, immune status, and HCV infection among Saudi haemodialysis patients.
2. Patients & Methods
2.1. Patients and Subjects
2.1.1. Haemodialysis Patients
All the haemodialysis patients in King Fahd Hospital of the University and King Fahd Medical Military Complex haemodialysis units from April 2008 to June 2010 were included in this study (N = 152). The sample included new and pre-registered cases.
2.1.2. Control Group
The control group (N = 160) was randomly selected from donors attending the blood bank from April 2008 to June 2010.
2.2. Statistical Analysis
Data were analyzed using SPSS version 16 statistical package. Correlation and association were derived using different parameters such as bivariate and multivariate analysis. A P-value of less than 0.05 was considered significant.
2.3. Ethical Consideration
This study was approved by the University of Dammam Ethical Committee. Signed written informed consent was obtained from each participant.
2.4. Procedures
2.4.1. HCV Detection and Quantitation
For HCV antibody detection, the collected sera was tested by Axsym hepatitis C virus enzyme immunoassay 3.0 (HCV EIA) (Abbott Laboratories, Abbott Park, IL, 60064), and a recombinant immunoblot assay (RIBA) HCV 3.0 SIA (Chiron Corporation, Emeryville, CA 94608). Positive sera by both ELISA and RIBA was tested by PCR (Amplicor Roche Diagnostic System, Hoffman Laroche, Basle, Switzerland) using primers selected from the highly conserved 5’-UTR of HCV genome. Amplified products were electrophoresed, photographed and analyzed. Extraction and amplification of RNA was carried out according to the manufacturer’s recommenda- tions. Quantitation of viral load was performed using Amplicor Roche Diagnostic System, (Hoffman Laroche, Basle, Switzerland) and this was carried out according to the manufacturer’s recommendations.
2.4.2. HCV Genotyping
Reverse Transcription-polymerase chain reaction (RT-PCR) using specific primers from 5’-UTR was performed, and the amplified products was genotyped/subtyped using commercial reverse phase hyperdization technique (Innolipa HCV, Innogenetics, Belgium) according to Simmond’s classification [18] .
2.4.3. HLA Typing
The HLA typing was done using DNA HLA typing procedures (PCR-sequence specific primers; PCR-SSP; One Lambda, Inc.).
2.4.4. Lymphocyte Markers
Whole blood samples were used. The following lymphocyte cell markers were analyzed: CD3, CD4, CD8, CD16, and CD19 using Flowcytometry (BD, USA). Values obtained from test groups were compared with normal range.
2.4.5. Cytokine Pattern
Serum levels of interleukin 2 and 10, interferon γ and TNFα was assessed using ELISA kits (Binding Sites, UK).
3. Results
3.1. Patients and Controls
A total of 152 patients and 160 controls were recruited from hospitals in the Eastern Province of Saudi Arabia. Patients included hemodialysis and nephrology unit patients, including kidney transplant patients. The mean age of the patients was 48 years of age. They included 91 males and 61 females. The period of hemodialysis varied between 3 months to 5 years. The control group included blood donors attending the Blood Bank (mean age 38; 155 males and 5 females).
3.2. Analysis of HCV Infection in Patients and Controls
From the total of 152 patients, 27 were found to have HCV antibodies as determined by EIA (17.8%). The seropositivity of these samples was confirmed by RIBA and RT-PCR. The average viral load was found to be 15 × 103 HCV RNA per 10 μl of plasma.
The pattern of HCV genotypes in the 27 HCV positive patients was genotype 4:14 (51.9%); genotype 1:11 (40.7%) and genotype 5a: 2 (7.4%). None of the subjects in the control groups tested positive for HCV antibodies or RNA.
3.3. Determination of HLA Types in HCV-Positive Patients and Controls
The 27 HCV-positive patients and 30 age and sex matched controls were screened for HLA-class I and HLA class II were screened in by polymerase chain reaction using sequence specific primers (PCR/SSP) method. The following types were detected: HLA-A9, A11, A19, A32, B5, B7, B8, B15, B17, B27, B35, B40, Bw6, Cw6, Cw7, DR1, DR2, DR3, DPB1, DQ1, DQB1, DRB1, DQP1, DR4, DR7, DR10, DR15, DR16, DR51 and DR53 (Table 1).
B7, CW6, CW7, and DR7 showed the highest gene frequencies of 22.1%, 17%, 9.2% and 26.9%, respectively. However, haplotypes A9-B7, A11-B15, A32-B5, A12-B27, A19-Bw6, A32-B27 and B35-Cw7 showed the highest frequencies.
3.4. Analysis of Cytokines and CD Molecules in Test and Control Groups
Measurements of cytokines Interleukin-2, 10, IFN-γ and TNFα showed that IL-2 level was 17.65 - 20.3 pg/l; IFN-γ was 22.15 - 41.1 pg/l; TNF-α was 2.5 - 5.6 pg/mL and IL-10 was 10.7 - 14.8 pg/mL (Table 2). No statistically significant difference was found between cytokine levels in the test and control groups (p > 0.05).
Measurement of CD markers (Table 3) showed the level of CD3 cells were between 1000 - 4000 µL; CD4 cells between 500 and 1600 µL; CD8 cells between 375 and 1100 µL; CD16 cells between 300 - 1530 µL and CD19 cells between 132 - 523 µL. No statistically significant difference was found between levels of different cells in the test and control groups (p > 0.05).
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Table 1. Frequency of HLA class I and class II in test and controls.
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Table 2. Cytokine levels (mean ± SD).
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Table 3. CD molecules levels (mean ± SD).
3.5. Correlation between HCV Infection and HLA Type and Immune Status in the Haemodialysis Patients
No correlation was found between HCV positive and the control groups in relation to cytokines and CD markers, between infection with HCV and HLA type, and the cytokines levels.
4. Discussion
Two studies, which were conducted by Al-Mugeiren et al. [19] and Soyannwo et al. [20] on pediatric and adult haemodialysis Saudi patients, revealed HCV infection rates of 11.2% and 50%, respectively. The HCV infection rate in the group of patients that we studied was 17.8%. This difference could be attributed to a number of factors, including the length of time that the patient groups had been receiving haemodialysis.
In addition, previous studies conducted on Saudi haemodialysis patients with HCV infection have shown that genotype 4 is the most prevalent with a range of 36% to 55%. In the present study, the most prevalent genotype was also found to be genotype 4 with a prevalence rate of 51%, which is in line with the previous studies. Other prevalent genotypes in our study were genotype 1 and 5a, which is also in line with other studies.
A study by White et al. [21] , which compared HLA types in the Gulf region, revealed that the prevalence of various HLA antigens differed significantly between various Gulf countries, including Oman, Kuwait and Saudi Arabia. The investigators also reported that HLA-DR2, which has the highest prevalence worldwide, had varying prevalence rates in the Gulf countries, with the Omani population having the highest prevalence of HLA- DR2. However, HLA-A, -B, -C and DR loci antigen frequencies were determined by Sheth et al. [22] on normal Saudi subjects showed that B21, CW4, CW7, and Dr7 had the highest frequencies, which is comparable to the present results.
A number of studies have reported a possible association between HLA genotype and HCV infection. Hadhoud et al. [23] studied this association in 146 HCV positive renal transplantation patients and 122 HCV negative individuals and reported a possible association between the expression of HLA alleles and HCV infection. Another study, by Fitzmaurice et al. [24] , examined the relationship in a cohort of HCV patients and reported a potential protective impact of HLA-A on HCV infection. Cangussu et al. [25] reported that polymorphic residues presented on different DRB1*11 and/or DQB1*03 alleles were found to be responsible for resistance against HCV infection.
In the present study no association was found between HLA genotype and HCV infection in the Saudi cohort studied. This discrepancy could be due to the small sample size included in this study. To confirm whether there is an association a larger sample size needs to be included.
5. Summary, Suggestions and Recommendations
The prevalence of HCV infection and its association with HLA genotype were identified in 152 Saudi hemo- dialysis patients and in 160 controls. The level of cytokines and immune cells in the same cohort were studied. No association was found between different HLA alleles and HCV infection in this population. A larger multicentre study should be conducted to ascertain these results.
Acknowledgments
This work was partially supported by a grant from the National Plan for Science and Technology, King Abdulaziz City for Science and Technology.
NOTES
*Corresponding author.