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Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells

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DOI: 10.4236/abb.2013.412135    6,181 Downloads   8,946 Views   Citations


Three transfection reagents, Lipofectamine® 2000, TransIT-PRO® and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO® was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.

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The authors declare no conflicts of interest.

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Sou, S. , Polizzi, K. and Kontoravdi, C. (2013) Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells. Advances in Bioscience and Biotechnology, 4, 1013-1019. doi: 10.4236/abb.2013.412135.


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