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Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells

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DOI: 10.4236/abb.2013.412135    6,181 Downloads   8,946 Views   Citations

ABSTRACT

Three transfection reagents, Lipofectamine® 2000, TransIT-PRO® and linear 25 kDa polyethylenimine were evaluated for transient expression of enhanced green fluorescent protein in Chinese hamster ovary cells. TransIT-PRO® was found to be more efficient under the examined conditions, but comes at an increased cost compared to the widely used PEI.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Sou, S. , Polizzi, K. and Kontoravdi, C. (2013) Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells. Advances in Bioscience and Biotechnology, 4, 1013-1019. doi: 10.4236/abb.2013.412135.

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