Share This Article:

Conditional Replication Adenovirus Sensitizes A549 Cancer Cell to Cisplatin

Abstract Full-Text HTML Download Download as PDF (Size:109KB) PP. 165-166
DOI: 10.4236/eng.2012.410B043    3,235 Downloads   4,096 Views  


Objective: The aim of the study was to improve the therapeutic effect for lung cancer using a synergetic strategy of adenovirus-based gene therapy combined with chemotherapy. Methods: A conditional replication adenovirus(CRAd) was employed to treat the A549 lung cancer cells and cisplatin-resisted A549(A549-DDP) cells. In vitro MTS / PMS assay were used to evaluate the cell viability. PCR were used to detect expression of Coxsackie receptor (CAR) and Multidrug resistance(MDR) gene. The in vivo anti-tumor effect of CRAd and cisplatin was evaluated using a subcutaneous mouse model. Results: The CRAd sensitizes A549 cancer cells to cisplatin. The mechanism of enhanced cell growth inhibition is associated with the increased CAR and MDR expression. Conclusion: Our approach is better than the conventional gene therapy and chemotherapy strategy.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Y. Liu and Y. Huang, "Conditional Replication Adenovirus Sensitizes A549 Cancer Cell to Cisplatin," Engineering, Vol. 4 No. 10B, 2012, pp. 165-166. doi: 10.4236/eng.2012.410B043.


[1] Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin.2012:62(1):10-29.
[2] Socinski MA, Bogart JA. Limited-stage small-cell lung cancer: the current status of combined-modality therapy. J Clin Oncol 2007,25: 4137-4145.
[3] Wan L,Hyo-Im C,Mi-Jin K, et a.l Depletion ofmitochondrial DNA up-regulates the expression of MDR1 gene via an increase in mRNA stability.Exp.Mo.lMed, 2008, 40(1): 109-117.
[4] Paredes Lario A,Blanco Garcia C et al.Expression of proteins associated with multidrug resistance and resistance to chemotherapy in lung cancer[J].Arch Bronconeumol,2007;43(9):479一484.
[5] Pujol JL,Simony J,Gautier V,et al.Immuno-histochemical study of p-glycoprotein distribution in lung [J].Cancer,1993,10:1.

comments powered by Disqus

Copyright © 2019 by authors and Scientific Research Publishing Inc.

Creative Commons License

This work and the related PDF file are licensed under a Creative Commons Attribution 4.0 International License.