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Characterization of purified β-glucosidase produced from Trichoderma viride through bio-processing of orange peel waste

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DOI: 10.4236/abb.2013.410124    2,978 Downloads   4,672 Views   Citations

ABSTRACT

In the present study, solid state fermentation was carried out using orange peel waste to produce β-glucosidase from Trichoderma viride. A locally isolated fungal strain T. viride was cultured in the solid state medium of orange peel (50% w/w moisture) under optimized fermentation conditions and maximum activity of 515 ± 12.4 U/mL was recorded after 4th day of incubation at pH 5.5 and 30℃. Indigenously produced β-glucosidase was subjected to the ammonium sulfate precipitation and Sephadex-G-100 gel filtration chromatography. In comparison to the crude extract β-glucosidase was 5.1-fold purified with specific activity of 758 U/mg. The enzyme was shown to have a relative molecular weight of 62 kDa as evidenced by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The purified β-glucosidase displayed 6 and 60℃ as an optimum pH and temperature respectively. 

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Irshad, M. , Anwar, Z. , Ramzan, M. , Mahmood, Z. and Nawaz, H. (2013) Characterization of purified β-glucosidase produced from Trichoderma viride through bio-processing of orange peel waste. Advances in Bioscience and Biotechnology, 4, 941-944. doi: 10.4236/abb.2013.410124.

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