Houttuynia cordata Thunb: A Review of Phytochemistry and Pharmacology and Quality Control

Jiangang Fu, Ling Dai, Zhang Lin, Hongmei Lu

College of Chemistry and Chemical Engineering, Central South University, Changsha, China.

DOI: 10.4236/cm.2013.43015                

Abstract

Houttuynia cordata Thunb is an important medicinal plant widely distributed in East Asia. The collected information is an attempt to cover recent developments in the pharmacology, phytochemistry and quality control of this species. During the past several decades, the medicinally important phyto-constituents have been identified including essential oil, flavonoids and other polyphenols, fatty acids and alkaloids. A survey of the literatures shows H. cordata possesses a variety of pharmacological activities including antiviral, antitumor, antimicrobial, anti-inflammatory, and antioxidative effects. Little attempt has been done to review the techniques used for its quality control. Future efforts should concentrate more on in vitro, in vivo studies and clinical trials in order to confirm traditional wisdom in the light of a rational phytotherapy. The information summarized here is intended to serve as a reference tool to practitioners in the fields of ethnopharmacology and natural products chemistry.

Keywords

Houttuynia cordata Thunb; Phytochemical Constituents; Pharmacological Activity; Quality Control

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	P. Drasar and J. Moravcova, “Recent Advances in Analysis of Chinese Medical Plants and Traditional Medicines,” Journal of Chromatography B, Vol. 812, No. 1-2, 2004, pp. 3-21.
[2]	N. R. Farnsworth, O. Akerele, A. S. Bingel, D. D. Soejarto and Z. Guo, “Medicinal Plants in Therapy,” Bulletin of the World Health Organization, Vol. 63, No. 6, 1985, pp. 965-981.
[3]	S. Frantz, “Drug Discovery: Playing Dirty,” Nature, Vol. 437, No. 7061, 2005, pp. 942-943. doi:10.1038/437942a
[4]	H. Zheng, Z. Dong and J. She, “Modern Study of Traditional Chinese Medicine,” Xue Yuan Press, Beijing, 1998.
[5]	K.-M. Lau, K.-M. Lee, C.-M. Koon, C. S.-F. Cheung, C.-P. Lau, H.-M. Ho, M. Y.-H. Lee, S. W.-N. Au, C. H.-K. Cheng, C. B.-S Lau, et al., “Immunomodulatory and Anti-SARS Activities of Houttuynia Cordata,” Journal of Ethnopharmacology, Vol. 118, No. 1, 2008, pp. 79-85. doi:10.1016/j.jep.2008.03.018
[6]	H. Lu, Y. Liang, L. Yi and X. Wu, “Anti-Inflammatory Effect of Houttuynia cordata Injection,” Journal of Ethnopharmacology, Vol. 104, No. 1-2, 2006, pp. 245-249. doi:10.1016/j.jep.2005.09.012
[7]	E. Park, S. Kum, C. Wang, S. Y. Park, B. S. Kim and G. Schuller-Levis, “Anti-Inflammatory Activity of Herbal Medicines: Inhibition of Nitric Oxide Production and Tumor Necrosis Factor-Alpha Secretion in an Activated Macrophage-Like Cell Line,” American Journal of Chinese Medicine, Vol. 33, No. 3, 2005, pp. 415-424. doi:10.1142/S0192415X05003028
[8]	I. S. Kim, J.-H. Kim, J. S. Kim, C.-Y. Yun, D.-H. Kim and J.-S. Lee, “The Inhibitory Effect of Houttuynia cordata Extract on Stem Cell Factor-Induced HMC-1 Cell Migration,” Journal of Ethnopharmacology, Vol. 112, No. 1, 2007, pp. 90-95. doi:10.1016/j.jep.2007.02.010
[9]	G. Z. Li, O. H. Chai, M. S. Lee, E.-H. Han, H. T. Kim and C. H. Song, “Inhibitory Effects of Houttuynia cordata Water Extracts on Anaphylactic Reaction and Mast Cell Activation,” Biological & Pharmaceutical Bulletin, Vol. 28, No. 10, 2005, pp. 1864-1868. doi:10.1248/bpb.28.1864
[10]	K. Hayashi, M. Kamiya and T. Hayashi, “Virucidal Effects of the Steam Distillate from Houttuynia cordata and Its Components on HSV-1, Influenza Virus, and HIV,” Planta Medica, Vol. 61, No. 3, 1995, pp. 237-241. doi:10.1055/s-2006-958063
[11]	L.-C. Chiang, J.-S. Chang, C.-C. Chen, L.-T. Ng and C.-C. Lin, “Anti-Herpes Simplex Virus Activity of Bidens pilosa and Houttuynia cordata,” American Journal of Chinese Medicine, Vol. 31, No. 3, 2003, pp. 355-362. doi:10.1142/S0192415X03001090
[12]	J.-S. Chang, L.-C. Chiang, C.-C. Chen, L.-T. Liu, K.-C. Wang and C.-C. Lin, “Antileukemic Activity of Bidens pilosa L. var. minor (Blume) Sherff and Houttuynia cordata Thunb,” American Journal of Chinese Medicine, Vol. 29, No. 2, 2001, pp. 303-312. doi:10.1142/S0192415X01000320
[13]	L.-T. Ng, F.-L. Yen, C.-W. Liao and C.-C. Lin, “Protective Effect of Houttuynia cordata Extract on BleomycinInduced Pulmonary Fibrosis in Rats,” American Journal of Chinese Medicine, Vol. 35, No. 3, 2007, pp. 465-475. doi:10.1142/S0192415X07004989
[14]	Y.-Y. Chen, J.-F. Liu, C.-M. Chen, P.-Y. Chao and T.-J. Chang, “A Study of the Antioxidative and Antimutagenic Effects of Houttuynia cordata Thunb. Using an Oxidized Frying Oil-Fed Model,” Journal of Nutritional Science and Vitaminology, Vol. 49, No. 5, 2003, pp. 327-333. doi:10.3177/jnsv.49.327
[15]	S.-K. Kim, S. Y. Ryu, J. No, S. U. Choi and Y. S. Kim, Cytotoxic Alkaloids from Houttuynia cordata,” Archives of Pharmacal Research, Vol. 24, No. 6, 2001, pp. 518-521. doi:10.1007/BF02975156
[16]	R. Bauer, A. Proebstle and H. Lotter, “Cyclooxygenase Inhibitory Constituents from Houttuynia cordata,” Phytomedicine, Vol. 2, No. 4, 1996, pp. 305-308. doi:10.1016/S0944-7113(96)80073-0
[17]	S.-Y. Shim, Y.-K. Seo and J.-R. Park, “Down-Regulation of Fc.vepsiln.RI Expression by Houttuynia cordata Thunb Extract in Human Basophilic KU812F Cells,” Journal of Medicinal Food, Vol. 12, No. 2, 2009, pp. 383-388. doi:10.1089/jmf.2007.0684
[18]	N. Nuengchamnong, K. Krittasilp and K. Ingkaninan, “Rapid Screening and Identification of Antioxidants in Aqueous Extracts of Houttuynia cordata Using LC-ESIMS Coupled with DPPH Assay,” Food Chemistry, Vol. 117, No. 4, 2009, pp. 750-756. doi:10.1016/j.foodchem.2009.04.071
[19]	H. Masuzawa, “The Diuretic Action of the Extract of Phytolacca Root (Phytolacca esculenta) and of Extracts of Some Plants and Drugs. II. The Diuretic Action of Some Plants and Drugs,” Journal of Okayama Medical Association, Vol. 52, No. 1, 1940, pp. 1813-1821.
[20]	S. Oyama, “Extraction of an Active Principle from Houttuynia cordata,” JP Patent No. 25000880, 1950.
[21]	G. Inagak, “Houttuynia cordata Extracts for Treatment of Athlete’s Foot,” JP Patent No. 53050313, 1978.
[22]	Y. Isogai, “An Antimicrobial Substance Isolated from the Rhizome of Houttuynia cordata,” Scientific Papers of the College of General Education, University of Tokyo, Tokyo, 1952.
[23]	H. Chikane, S. Yuka and Y. Takao, “Antibacterial Activity of Extracts from Houttuynia cordata and It’s Components,” Bulletin of Saitama Medical School Junior College, Vol. 14, No. 2, 2003, pp. 1-6.
[24]	C. Lee and S.-J. Lin, “Constituents of Houttuynia cordata,” Beiyi Xuebao, Vol. 6, No. 1, 1974, pp. 75-78.
[25]	K.-I. Hoshishima and H. Okabe, “Promoting Agents Obtained from Houttuynia cordata for the Production of an Antibiotic Substance by a Strain of Gram-Positive, SporeBearing Bacilli,” Tohoku Journal of Experimental Medicine, Vol. 52, No. 3, 1950, pp. 265-271.
[26]	C. P. Kala, “Ethnomedicinal Botany of the Apatani in the Eastern Himalayan Region of India,” Journal of Ethnobiology and Ethnomedicine, Vol. 1, No. 11, 2005, pp. 11-18. doi:10.1186/1746-4269-1-11
[27]	J. W. Kim, H. J. Ji, J. H. Ahn and M. S. Kim, “Cosmetic Composition Containing Herbal Medicine Extracts for Preventing Wrinkle,” KR Patent No. 2009002678, 2009.
[28]	M. Ida, “Cosmetics,” JP Patent No. 54145226, 1979.
[29]	T. Tehara, “Antiaging Cosmetics Containing Natural Products,” JP Patent No. 2006241036, 2006.
[30]	Y. S. You, “Houttuynia cordata Face Lotion,” KR Patent No. 2001016594, 2001.
[31]	M. Araki, “Additives for Cosmetics to Improve the Skin Conditions,” JP Patent No. 2007246516, 2007.
[32]	S. H. So and Y. O. Kim, “Cosmetic Composition for Alleviating Skin Inflammation,” KR Patent No. 2009-049401, 2009.
[33]	H. M. Kim, H. J. Kim, S. G. Park, J. J. Kim and J. H. Choi, “Cosmetic Composition for Alleviating Skin Trouble,” KR Patent No. 2009002678, 2009.
[34]	N. Takagi, M. Kamiya and K. Yoshida, “Cosmetics Containing Rhinacanthus nasuta Extracts, Ganoderma lucidum Extracts and/or Houttuynia cordana Extracts for Skin Aging Control and Hair Protection,” JP Patent No. 09143025, 1997.
[35]	J. S. Choi, “Hair Care Product for Nourishing Hair and Preventing Dandruff and Method for Manufacturing the Same,” KR Patent No. 2004037057, 2004.
[36]	Y. S. You, “Method of Preparing Functional Massage Pack Useful for Treating Acne from Rice Powder, Houttuynia Cordata Thunb. And Green Tea Leaves,” KR Patent No. 2006059930, 2006.
[37]	T. H. Hoang, V. B. Ha, Q. H. Tran and V. H, Ha, “Antineoplastic Activity of Flavonoid Components Extracted from Leaves of Houttuynia cordata Thunb. in Vietnam,” Tap Chi Duoc Hoc, Vol. 51, No. 10, 2003, pp. 9-10.
[38]	C. W. Choi, S. C. Kim, S. S. Hwang, B. K. Choi, H. J. Ahn, M. Y. Lee, S. H. Park and S. K. Kim, “Antioxidant Activity and Free Radical Scavenging Capacity between Korean Medicinal Plants and Flavonoids by AssayGuided Comparison,” Plant Science, Vol. 163, No. 6, 2002, pp. 1161-1168. doi:10.1016/S0168-9452(02)00332-1
[39]	H. Zeng, L. Jiang and Y. Zhang, “Chemical Constituents of Volatile Oil from Houttuynia cordata Thunb,” Journal of Plant Resources and Environment, Vol. 12, No. 3, 2003, pp. 50-52.
[40]	J. Meng, X. Dong, Y. Zhou, Z. Jiang, S. Liang and Z. Zhao, “Orthogonal Experiment Using SFE-CO2 in Extraction of Essential Oil from Fresh Houttuynia cordata and Analysis of Essential Oil by GC-MS,” China Journal of Chinese Materia Medica, Vol. 32, No. 3, 2007, 215-217.
[41]	M. I. Ch, Y. F. Wen and Y. Cheng, “Gas Chromatographic/Mass Spectrometric Analysis of the Essential Oil of Houttuynia cordata Thunb by Using On-Column Methylation with Tetramethylammonium Acetate,” Journal of AOAC International, Vol. 90, No. 1, 2007, pp. 60-67.
[42]	X. Hao, L. Li, Z. Ding and Y. Yi, “Analysis of Essential Oil from Houttuynia cordata in Guizhou,” Acta Botanica Yunnanica, Vol. 17, No. 3, 1995, pp. 350-352.
[43]	Z. Zeng, Y. Liang and C. Xu, “Comparing Chemical Fingerprints of Herbal Medicines Using Modified Window Target-Testing Factor Analysis,” Analyical And Bioanalytical Chemistry, Vol. 381, No. 4, 2005, pp. 913-924
[44]	H.-D. Kwon, I.-H. Cha, W.-K. Lee, J.-H. Song and I.-H. Park, “Antibacterial Activity of Volatile Flavor Components from Houttuynia cordata Thunb,” Journal of Food Science and Nutrition, Vol. 1, No. 2, 1996, pp. 208-213.
[45]	J.-M. Kang, I.-H. Cha, Y.-K. Lee and H.-S. Ryu, “Identification of Volatile Essential Oil, and Flavor Characterization and Antibacterial Effect of Fractions from Houttuynia cordata Thunb. I. Identification of Volatile Essential Oil Compounds from Houttuynia cordata Thunb,” Han’guk Sikp’um Yongyang Kwahak Hoechi, Vol. 26, No. 2, 1997, pp. 209-213.
[46]	J.-M. Kang, I.-H. Cha, Y.-K. Lee and H.-S. Ryu, “Identification of Volatile Essential Oil, and Flavor Characterization and Antibacterial Effect of Fractions from Houttuynia cordata Thunb. II. Flavor Characterization and Antibacterial Effect of Fraction from Houttuynia cordata Thunb Obtained by Preparative HPLC,” Han’guk Sikp’um Yongyang Kwahak Hoechi, Vol. 26, No. 2, 1997, pp. 214-221.
[47]	L. Chen, W. Wu and Y. Zheng, “TLC Analysis on Essential Oil in Houttuynia cordata with Different Chromosome Numbers,” Chinese Traditional and Herbal Drugs, Vol. 35, No. 12, 2004, pp. 1399-1402.
[48]	Y.-L. Liu and Z.-F. Deng, “Study on the Chemical Constituents of the Essential Oil from Houttuynia cordata Thunb,” Chinese Bulletin of Botany, Vol. 21, No. 3, 1979, pp. 244-249.
[49]	M. Qi, X. Ge, M. Liang and R. Fu, “Flash Gas Chromatography for Analysis of Volatile Compounds from Houttuynia cordata Thunb,” Analytica Chimica Acta, Vol. 527, No. 1, 2004, pp. 69-72. doi:10.1016/j.aca.2004.08.073
[50]	H. Lu, “Fingerprint and Fingerprint-Efficacy Study of Houttuynia cordata Thunb. and Injection, a Traditional Chinese Medicine (TCM),” Central South University, Chemistry & Chemical Engineering College, Changsha, 2006.
[51]	C. Huang, W. Wu and Y. Zheng, “Analysis on the Chemical Constituents of Essential Oil from Different Parts of Houttuynia cordata Thunb,” Chinese Journal of Pharmaceutical Analysis, Vol. 27, No. 1, 2007, pp. 40-44.
[52]	W.-F. Yang, Y. Chen and Y.-Y. Cheng, “Essential Oils from Various Parts of Houttuynia cordata Thunb,” Chinese Traditional and Herbal Drugs, Vol. 37, No. 8, 2006, pp. 1149-1151.
[53]	H. Lu, X. Wu, Y. Liang and J. Zhang, “Variation in Chemical Composition and Antibacterial Activities of Essential Oils from Two Species of Houttuynia cordata Thunb,” Chemical & Pharmaceutical Bulletin, Vol. 54, No. 7, 2006, pp. 936-940. doi:10.1248/cpb.54.936
[54]	H. Lu, Y. Liang, X. Wu, L. Yi and S. Chen, “Comparative Study of Fingerprints of Houttuynia cordata Injection Made of Fresh and Dry Raw Material,” Chinese Journal of Analytical Chemistry, Vol. 34, No. 6, 2006, pp. 813-816.
[55]	H. Nakamura, T. Ota and G. Fukuchi, “The Constituents of Diuretic Drugs. II. The Flavonol Glucoside of Houttuynia cordata Thunb,” Journal of the Pharmaceutical Society of Japan, Vol. 56, No. 3, 1936, p. 68.
[56]	J. Meng, X. Dong, Z. Jiang, S. Leung and Z. Zhao, “Study on Chemical Constituents of Flavonoids in Fresh Herb of Houttuynia cordata,” China Journal of Chinese Materia Medica, Vol. 31, No. 16, 2006, pp. 1335-1337.
[57]	J. Meng, X. P. Dong, Y. S. Zhou, Z. H. Jiang, S.-Y. K. Leung and Z. Z. Zhen, “Studies on Chemical Constituents of Phenols in Fresh Houttuynia cordata,” China Journal of Chinese Materia Medica, Vol. 32, No. 10, 2007, pp. 929-931.
[58]	S.-C. Chou, C.-R. Su, Y.-C. Ku and T.-S. Wu, “The Constituents and Their Bioactivities of Houttuynia cordata,” Chemical & pharmaceutical Bulletin, Vol. 57, No. 11, 2009, pp. 1227-1230. doi:10.1248/cpb.57.1227
[59]	S. H. Li and X. J. Wu, “Studies on Flavonoids Extracting Methods in Different Parts of Houttunia cordata Thunb,” Journal of Jishou University, Vol. 28, No. 3, 2007, pp. 117-118.
[60]	Y. Zhang, S. Li and X. Wu, “Pressurized Liquid Extraction of Flavonoids from Houttuynia cordata Thunb,” Separation and Purification Technology, Vol. 58, No. 3, 2008, pp. 305-310. doi:10.1016/j.seppur.2007.04.010
[61]	D. S. Jang, J. M. Kim, Y. M. Lee, J. L. Yoo, Y. S. Kim, J.-H. Kim and J. S. Kim, “Flavonols from Houttuynia cordata with Protein Glycation and Aldose Reductase Inhibitory Activity,” Natural Product Sciences, Vol. 12, No. 4, 2006, pp. 210-213.
[62]	S. Huang, C. Wan, Y. Zou and R. Li, “Extraction and Identification of Flavones from Houttuynia cordata Thunb. by Ultrasonic Wave,” Li Shizhen Medicine And Materia Medica Research, Vol. 17, No. 11, 2006, pp. 2261-2262.
[63]	C. Ye, J. Kan, S. Tan, J. Fan and G. Yang, “Microwave Assisted Extraction of Total Flavonoids from Houttuynia cordata Thunb. Leaves,” China Brewing, No. 1, 2009, pp. 134-137.
[64]	C. Ye, J. Kan, S. Tan, Q. Zhu, L. Xu and G. Yang, “Extraction and Separation of Flavonoids from Houttuynia cordata Thunb. Leaves,” Transactions of the Chinese Society of Agricultural Engineering, Vol. 24, No. 10, 2008, pp. 227-232.
[65]	S. Y. Xu, J. X. Hong, G. M. Yan, Y. F. Lin and J. Y. Sun, “Study on Purification Process of Extract of the Fresh Houttuynia cordata,” Research and Practice on Chinese Medicines, Vol. 22, No. 2, 2008, pp. 55-58.
[66]	T. Zhang and D. Chen, “Anticomplementary Principles of a Chinese Multiherb Remedy for the Treatment and Prevention of SARS,” Journal of Ethnopharmacology, Vol. 117, No. 2, 2008, pp. 351-361. doi:10.1016/j.jep.2008.02.012
[67]	Y. Guo and J. Xu, “Extraction and Purification of Houttuyniae cordata Flavonoids and Identification of Flavonoids Type,” Food Science, Vol. 28, No. 9, 2007, pp. 287-291.
[68]	X. Xu, H. Ye, W. Wang, L. Yu and G. Chen, “Determination of Flavonoids in Houttuynia cordata Thunb. and Saururus chinensis (Lour.) Bail. by Capillary Electrophoresis with Electrochemical Detection,” Talanta, Vol. 68, No. 3, 2006, pp. 759-764. doi:10.1016/j.talanta.2005.05.027
[69]	K. H. Choe, S. J. Kwon and D. S. Jung, “A Study on Chemical Composition of Saururaceae Growing in Korea. 4. On Flavonoid Constituents of Houttuynia cordata,” Analytical Science & Technology, Vol. 4, No. 3, 1991, pp. 285-288.
[70]	J.-I. Fuse, H. Kanamori, I. Sakamoto and S. Yahara, “Flavonol Glycosides in Houttuynia cordata,” Natural Medicines, Vol. 48, No. 4, 1994, pp. 307-311.
[71]	Q. C. Eng, Z. N. Yang, J. W. Hu, W. S. Cheng and C. X. Li, “Determination of Contents of Seven Flavonoids in Houttuynia cordata Thunb by HPLC,” Journal of Jiangxi Normal University, Vol. 32, No. 6, 2008, pp. 645-648, 661.
[72]	L.-S. Wu, J.-P. Si and X.-Q. Yuan, “Quantitive Variation of Flavonoids in Houttuynia cordata from Different Geographic Origins in China,” Chinese Journal of Natural Medicines, Vol. 7, No. 1, 2009, pp. 40-45.
[73]	J. Meng, H. Liao, X. Dong and Z. Zhao, “Quantitative Analysis of Flavonoids in Freeze-Dried Houttuynia cordata from Different Habitats by HPLC-MS,” Journal of Guangdong College of Pharmacy, Vol. 24, No. 2, 2008, pp. 114-117.
[74]	J. Meng, K. S.-Y. Leung, Z. Jiang, X. Dong, Z. Zhao and L.-J. Xu, “Establishment of HPLC-DAD-MS Fingerprint of Fresh Houttuynia cordata,” Chemical & Pharmaceutical Bulletin, Vol. 53, No. 12, 2005, pp. 1604-1609. doi:10.1248/cpb.53.1604
[75]	J.-C. Park, J.-M. Hur, J.-G. Park, S.-J. Park, J.-H. Lee, N.-J. Sung, M.-R. Choi, S.-H. Song, M.-S. Kim and J.-W. Choi, “The Effects of Houttuynia cordata on the Hepatic Bromobenzene Metabolizing Enzyme System in Rats and Isolation of Phenolic Compounds,” Korean Journal of Pharmacognosy, Vol. 31, No. 2, 2000, pp. 228-234.
[76]	T. Kawamura, Y. Hisata, K. Okuda, Y. Noro, T. Tanaka, M. Yoshida and E. Sakai, “Pharmacognostical Studies of Houttuyniae Herba. (1) Flavonoid Glycosides Contents of Houttuynia cordata Thunb,” Natural Medicines, Vol. 48, No. 3, 1994, pp. 208-212.
[77]	E. Sakai, T. Shibata, T. Kawamura, Y. Hisata, Y. Noro, M. Yoshida and T. Tanaka, “Pharmacognostical Studies of Houttuyniae Herba: 2. Growth and Flavonoid Glycoside Contents of Houttuynia cordata Thunb. Cultivated under Shade Condition,” Natural Medicines, Vol. 50, No. 1, 1996, pp. 45-48.
[78]	H. Nishiya, K. Ishiwata, K. Komatsu, O. Nakata, K. Kitamura and S. Fujii, “Platelet Aggregation Inhibitors from Jyu-Yaku (Houttuyniae Herb),” Chemical & Pharmaceutical Bulletin, Vol. 36, No. 5, 1988, pp. 1902-1904. doi:10.1248/cpb.36.1902
[79]	T. T. Jong and M. Y. Jean, “Alkaloids from Houttuynia cordata,” Journal of the Chinese Chemical Society, Vol. 40, No. 3, 1993, pp. 301-303.
[80]	L. Wang, Y. Zhao, L. Zhou and J. Zhou, “Chemical Constituents of Houttuynia cordata,” Chinese Traditional and Herbal Drugs, Vol. 38, No. 12, 2007, pp. 1788-1790.
[81]	K. H. Choe, S. J. Kwon and K. C. Lee, “Chemical Composition of Saururaceae Growing in Korea. (3). On Fatty Acids and Amino Acids of Houttuynia cordata and Saururus chinensis,” Punsok Kwahak, Vol. 2, No. 2, 1989, pp. 285-292.
[82]	S. Takagi, M. Yamaki, K. Masuda and M. Kubota, “On the Constituents of the Terrestrial Part of Houttuynia cordata Thunb,” Shoyakugaku Zasshi, Vol. 32, No. 2, 1978, pp. 123-125.
[83]	X. Wu, S. Li, A. Li and J. Zhang, “Chemical Components from Herba of Houttuynia cordata Thunb,” Journal of Chinese Medicinal Materials, Vol. 31, No. 8, 2008, pp. 1168-1170.
[84]	T. T. Jong and M. Y. Jean, “Constituents of Houttuyniae cordata and the Crystal Structure of Vomifoliol,” Journal of the Chinese Chemical Society, Vol. 40, No. 4, 1993, pp. 399-402.
[85]	S.-C. Chou, “The Constituents of the Houttuynia cordata Thumb,” National Cheng Kung University, Tainan, 2005.
[86]	M. Mori, K. Suzuki, Y. Tsukahara and M. Nada, “Changes in the Components of Houttuynia cordata during Growth,” Sagami Joshi Daigaku Kiyo, Shizenkei, Vol. 59B, No. 3, 1995, pp. 89-95.
[87]	X. Gong and M. Cheng, “Analysis and Appraisal of Nutrients of Houttuynia cordata Thunb,” Food and Fermentation Industries, Vol. 30, No. 11, 2004, pp. 102-105.
[88]	Y. Kimura and Y. Nishikawa, “Standardization of Crude Drugs. III. 2. Component of Houttuynia cordata,” Journal of the Pharmaceutical Society of Japan, Vol. 73, No. 47, 1953, pp. 196-198.
[89]	T. Ohta, “The Diuretic Components of Houttuynia cordata,” Journal of the Pharmaceutical Society of Japan, Vol. 62, No. 2, 1942, pp. 105-106.
[90]	W. Zhang, F. Lu, S. Pan and S. Li, “Extraction of Volatile Oil from Houttuynia cordata and Its Anti-Biotic and Anti-Virus Activities,” Practical Preventive Medicine, Vol. 15, No. 2, 2008, pp. 312-316.
[91]	J. Meng, X. Zong and X. Dong, “Study on Pharmacological Effects of Fresh and Dry Houttuynia cordata Thunb,” Li Shizhen Medicine and Materia Medica Research, Vol. 19, No. 6, 2008, pp. 1315-1316.
[92]	J. Zhang, X. Wu, Z. Luo and X. Zhong, “Determination of Chemical Components and Antibacterial Activities of Houttuynia cordata Thunb. during Desiccation and Storage,” Food Science, Vol. 28, No. 11, 2007, pp. 565-569.
[93]	M. Fu, X. Wu, D. Liu, J. Zhang, L. Wei and X. Jiang, “Research on Antibacterial Activity in Vitro of the Houttuynia cordata Thunb Injection,” Journal of Anhui Agricultural Science, Vol. 35, No. 8, 2007, pp. 2264-2265.
[94]	P. Qian, Y. Liang and H. Lu, “The Study of Antibacterial Activity in Quality Control of Houttuynia cordata Injection,” Journal of Xiangtan Normal University, Vol. 29, No. 3, 2007, pp. 12-14.
[95]	Y. Hou and X. Zhang, “Antiphlogistic Action of Houttuynia cordata Injection in Vitro and in Mice,” China Journal of Chinese Materia Medica, Vol. 15, No. 4, 1990, pp. 221-222, 255.
[96]	T. Kosuge and H. Okeda, “Nonylpyrazole, a New Antimicrobial Substance,” Journal of Biochemistry, Vol. 41, No. 2, 1954, pp. 183-186.
[97]	G. Kim, D. Kim and J. Lim, “Biological and Antibacterial Activities of the Natural Herb Houttuynia cordata Water Extract against the Intracellular Bacterial Pathogen Salmonella within the RAW 264.7 Macrophage,” Biological & Pharmaceutical Bulletin, Vol. 31, No. 11, 2008, pp. 2012-2017. doi:10.1248/bpb.31.2012
[98]	S. B. Bharate, “Medicinal Plants with Anti-HIV Potential,” Journal of Medicinal and Aromatic Plant Sciences, Vol. 25, No. 2, 2003, pp. 427-440.
[99]	M. Shimura, Y. Zhou, Y. Asada, T. Yoshikawa, K. Hatake, F. Takaku and Y. Ishizaka, “Inhibition of VprInduced Cell Cycle Abnormality by Quercetin: A Novel Strategy for Searching Compounds Targeting Vpr,” Biochemical and Biophysical Research Communications, Vol. 261, No. 2, 1999, pp. 308-316. doi:10.1006/bbrc.1999.0994
[100]	N. Morita, K. Hayashi, A. Fujita and H. Matsui, “Extraction of Antiviral Substances from Houttuynia cordata Thunb,” JP Patent No. 07118160, 1995.
[101]	Y. Yan, X. Chen, X. Yang, S. Wu, D. Fang and C. Dong, “Cooperative Anti-Influenza Virus Activities of Amantadine, Ribavirin and Herb Houttuynia,” Virologica Sinica, Vol. 17, No. 2, 2002, pp. 192-194.
[102]	D. H. Kwon, W. J. Choi, C. H. Lee, J. H. Kim and M. B. Kim, “Flavonoid Compound Having an Antiviral Activity,” WO Patent No. 2007069823, 2007.
[103]	H.-J. Choi, J.-H. Kim, C.-H. Lee, Y.-J. Ahn, J.-H. Song, S.-H. Baek and D.-H. Kwon, “Antiviral Activity of Quercetin 7-Rhamnoside against Porcine Epidemic Diarrhea Virus,” Antiviral Research, Vol. 81, No. 1, 2009, pp. 77-81.
[104]	X. Zheng, X. Tang and X. Su, “Experimental Study of Inhibitory Effect of the Four Traditional Chinese Herb Medicines on Epidemic Hemorrhagic Fever Virus,” Bulletin of Hunan Medical University, Vol. 18, No. 2, 1993, pp. 165-167.
[105]	T.-Y. Lin, Y.-C. Liu, J.-R. Jheng, H.-P. Tsai, J.-T. Jan, W.-R. Wong and J.-T. Horng, “Anti-Enterovirus 71 Activity Screening of Chinese Herbs with Anti-Infection and Inflammation Activities,” American Journal of Chinese Medicine, Vol. 37, No. 1, 2009, pp. 143-158.
[106]	X. Kou, “Traditional Chinese Medicine Composition Containing Taraxacum and Glycyrrhiza and Others for Treating Lung Cancer,” CN Patent No. 101406657, 2009.
[107]	Q. Li, “Chinese Medical Honeyed Pill for Treating Lung Carcinoma,” CN Patent No. 101391036, 2009.
[108]	H. Jung, J. Choi and C. Jin, “Effects of Flos Lonicerae and Herba Houttuyniae on Human Cancer Cell-Lines,” Korean Journal of Oriental Physiology & Pathology, Vol. 10, No. 3, 1996, pp. 126-132.
[109]	W. Bae, H. Go and C. Kim, “Experimental Study on the Effect of Houttuyniae Herbal Accupuncture on the Growth of Melanoma B16 in Mice,” The Korean Acupuncture and Moxibustion Journal, Vol. 18, No. 2, 2001, pp. 186-201.
[110]	K.-B. Kwon, E.-K. Kim, B.-C. Shin, E.-A. Seo, J.-Y. Yang and D.-G. Ryu, “Herba Houttuyniae Extract Induces Apoptotic Death of Human Promyelocytic Leukemia Cells via Caspase Activation Accompanied by Dissipation of Mitochondrial Membrane Potential and Cytochrome c Release,” Experimental and Molecular Medicine, Vol. 35, No. 2, 2003, pp. 91-97.
[111]	D. S. Hah, C. H. Kim, J.-D. Ryu, E. K. Kim and J. S. Kim, “Evaluation of Protective Effects of Houttuynia cordata on H2O2-Induced Oxidative DNA Damage Using an Alkaline Comet Assay in Human HepG2 Cells,” Journal of Toxicology and Public Health, Vol. 23, No. 1, 2007, pp. 25-31.
[112]	H. Ha, D. Y. Jung and S. W. Park, “Anticancer Effect of Houttuynia cordata Extract on Cancered ICR Mouse and L1210 Cells with Changes of SOD and GPx Activities,” Yakhak Hoechi, Vol. 48, No. 4, 2004, pp. 219-225.
[113]	A. Murakami, S. Jiwajinda and K. Koshimizu, “Screening for in Vitro Anti-Tumor Promoting Activities of Edible Plants from Thailand,” Cancer Letters, Vol. 95, No. 1-2, 1995, pp. 139-146.
[114]	Y.-Y. Chen, C.-M. Chen, P.-Y. Chao, T.-J. Chang and J.-F. Liu, “Effects of Frying Oil and Houttuynia cordata Thunb on Xenobiotic-Metabolizing Enzyme System of Rodents,” World Journal of Gastroenterology, Vol. 11, No. 3, 2005, pp. 389-392.
[115]	H. Fan, W. Qu, Y. Li and M. Sun, “Experimental Investigation for Anti-Tumor Activity of Flavonoid from the Houttuynia cordata Thunb. in Vitro,” Chinese Journal of Hospital Pharmacy, Vol. 28, No. 7, 2008, pp. 528-531.
[116]	J. Meng, D. He, Y. Zhou and X. Dong, “Comparison on the Pharmacological Effects of Different Extract Parts from Houttuynia cordata Thunb,” Li Shizhen Medicine And Materia Medica Research, Vol. 19, No. 5, 2008, pp. 1050-1051.
[117]	Q. Feng, Y. Ren, Y. Wang, H. Ma, J. Xu, C. Zhou, Z. Yin and L. Luo, “Anti-Inflammatory Effect of SQC-Beta-CD on Lipopolysaccharide-Induced Acute Lung Injury,” Journal of Ethnopharmacology, Vol. 118, No. 1, 2008, pp. 51-58.
[118]	J. Hong, B. Wang, Y. Gao, B. Feng, J. Mo, Y. Wang, H. Yao, X. Xu, W. Zhao and F. Tang, “Effect of Fresh Herba Houttuyniae Extract on Lipopolysaccharide-Induced Inflammation in Mice,” The Chinese Journal of Modern Applied Pharmacy, Vol. 25, No. 5, 2008, 376-378.
[119]	J. Lee, I. Kim, J. Kim, J. Kim, D. Kim and C. Yun, “Suppressive Effects of Houttuynia cordata Thunb (Saururaceae) Extract on Th2 Immune Response,” Journal of Ethnopharmacology, Vol. 117, No. 1, 2008, pp. 34-40.
[120]	A. Probstle and R. Bauer, “Aristolactams and a 4,5-Dioxoaporphine Derivative from Houttuynia cordata,” Planta Medica, Vol. 58, No. 6, 1992, pp. 568-569.
[121]	K. Taguchi, Y. Hagiwara, K. Kajiyama and Y. Suzuki, “Pharmcological Studies of Houttuyniae Herba: The Antiinflammatory Effect of Quercitrin,” Journal of the Pharmaceutical Society of Japan, Vol. 113, No. 4, 1993, pp. 327-333.
[122]	X.-L. Jiang and H.-F. Cui, “Different Therapy for Different Types of Ulcerative Colitis in China,” World Journal of Gastroenterology, Vol. 10, No. 10, 2004, pp. 1513-1520.
[123]	H.-M. Lu, Y.-Z. Liang and P. Qian, “Profile-Effect on Quality Control of Houttuynia cordata Injection,” Acta pharmaceutica Sinica, Vol. 40, No. 12, 2005, pp. 1147-1150.
[124]	H.-M. Lu, Y.-Z. Liang, X.-J. Wu and P. Qiu, “Tentative Fingerprint-Efficacy Study of Houttuynia cordata Injection in Quality Control of Traditional Chinese Medicine,” Chemical & Pharmaceutical Bulletin, Vol. 54, No. 5, 2006, pp. 725-730.
[125]	Y.-C. Kim and S.-K. Chung, “Reactive Oxygen Radical Species Scavenging Effects of Korean Medicinal Plant Leaves,” Food Science and Biotechnology, Vol. 11, No. 4, 2002, pp. 407-411.
[126]	E. Cho, T. Yokozawa, D. Rhyu, S. Kim, N. Shibahara and J. Park, “Study on the Inhibitory Effects of Korean Medicinal Plants and Their Main Compounds on the 1,1-diphenyl-2-picrylhydrazyl Radical,” Phytomedicine, Vol. 10, No. 6-7, 2003, pp. 544-551.
[127]	E. J. Cho, T. Yokozawa, D. Y. Rhyu, H. Y. Kim, N. Shibahara and J. C. Park, “The Inhibitory Effects of 12 Medicinal Plants and Their Component Compounds on Lipid Peroxidation,” American Journal of Chinese Medicine, Vol. 31, No. 6, 2003, pp. 907-917.
[128]	H. Wang and Y. Xiu, “Effect of Houttuynia cordata on Urinary Albumin and Insulin Resistance of Diabetes Mellitus Rats,” Traditional Chinese Drug & Clinical Pharmacology, Vol. 19, No. 1, 2008, pp. 12-14.
[129]	H. Wang, Y. Xiu and K. Sun, “Effect of Houttuynia cordata on Fat Metabolism in Plasma and Insulin Resistance of Diabetes Mellitus Rats,” Chinese Journal of Basic Medicine in Traditional Chinese Medicine, Vol. 15, No. 1, 2009, pp. 72-73, 78.
[130]	F. Wang, F. Lu, G. Chen and L. Xu, “Influence of Houttuynia cordata Thumb on Expression of TGF-β1 and HGF in Kidney Tissue of Streptozotocin Induced Diabetic Rats,” The 3rd World Integrative Medicine Congress Abstracts, Guangzhou, 21-24 September 2007, p. 722.
[131]	F. Wang, F. Lu and L. Xu, “Effects of Houttuynia cordata Thumb on Expression of BMP-7 and TGF-β1 in the Renal Tissues of Diabetic Rats,” Journal of Traditional Chinese Medicine, Vol. 27, No. 3, 2007, pp. 220-225.
[132]	Y. Kimata, Y. Eto, M. Asanoma, Y. Sakabe and H. Horitsu, “Inhibitory Actions of Chloroform Extracts from Natural Medicines on the Mutagenicity of Trp-P-2 and 1-NP,” Chukyo Joshi Daigaku Kenkyu Kiyo, Vol. 31, No. 2, 1997, pp. 123-130.
[133]	Z. Song, C. Wang, J. Cheng, F. Li, Z. Zhu, Y. Ning and M. Zhang, “Effect of Injection of Houttuynia cordata Thunb, Hyperiaum japonlcum Thunb and Erycibe obtusifoIia Benth on Immune Function of Rats,” Chinese Traditional and Herbal Drugs, Vol. 24, No. 12, 1993, pp. 643-644, 438.
[134]	J. Kim, C.-S. Park, Y. Lim and H.-S. Kim, “Paeonia japonica, Houttuynia cordata, and Aster scaber Water Extracts Induce Nitric Oxide and Cytokine Production by Lipopolysaccharide-Activated Macrophages,” Journal of Medicinal Food, Vol. 12, No. 2, 2009, pp. 365-373.
[135]	K.-M. Ji, M. Li, J.-J. Chen, Z.-K. Zhan and Z.-G. Liu, “Anaphylactic Shock and Lethal Anaphylaxis Caused by Houttuynia cordata Injection, a Herbal Treatment in China,” Allergy, Vol. 64, No. 5, 2009, pp. 816-817.
[136]	L. Lei, X. Pu, K. Yang, H. Wang, Y. Liu, D. Zhang and L. Zhang, “Adverse Response Caused by Intravenous Drip of Houttuynia cordata Injection I,” Pharmacology and Clinics of Chinese Materia Medica, Vol. 23, No. 5, 2007, pp. 131-133.
[137]	Y. Liu, X. Pu and L. Lei, “Adverse Response Caused by Intravenous Drip of Houttuynia cordata Injection Ⅱ,” Pharmacology and Clinics of Chinese Materia Medica, Vol. 24, No. 2, 2008, pp. 61-63.
[138]	China TPCo (Ed.), “The Chinese Pharmacopoeia,” China Medical Science Press, Beijing, 2010.
[139]	WHO “General Guidelines for Methodologies on Research and Evaluation of Traditional Medicines,” 2000, p. 1.
[140]	Y.-Z. Liang, P. Xie and K. Chan, “Quality Control of Herbal Medicines,” Journal of Chromatography B, Vol. 812, No. 1-2, 2004, pp. 53-70.
[141]	V. E. Tyler, “Phytomedicines: Back to the Future,” Journal of Natural Products, Vol. 62, No. 11, 1999, pp. 1589-1592.
[142]	WHO “Guidelines for the Assessment of Herbal Medicines,” 1991.
[143]	J. Wang, R. van der Heijden, S. Spruit, T. Hankermeier, K. Chan, J. van der Greef, G. Xu and M. Wang, “Quality and Safety of Chinese Herbal Medicines Guided by a Systems Biology Perspective,” Journal of Ethnopharmacology, Vol. 126, No. 1, 2009, pp. 31-41.
[144]	C. P. Commission (Ed.), “The Pharmacopoeia of the People’s Republic of China,” China Medical Science Press, Beijing, 2010.
[145]	China Mohotpsro, “Farther Standardization of Raw Materials Administrators for Health Foods,” 2002.
[146]	http://en.wikipedia.org/wiki/Houttuynia
[147]	Y. Wata, “Therapeutic Drinks from Houttuynia cordata,” JP Patent No.56097234, 1981.
[148]	K. Mori, “Deodorizing Fish Oil,” JP Patent No. 35240, 1919.
[149]	J. S. Choi, “Knife-Cut Noodles Using Houttuyniae Herba and Method for Preparing the Same,” KR Patent No. 2004063884, 2004.
[150]	S. H. Kwon and M. S. Kim, “Method for Manufacturing Soy Sauce by Using Houttuynia Cordata and Saururus Chinensis,” KR Patent No. 2009076250, 2009.
[151]	Y. S. You, “Method for Manufacturing Carbonic Drink of Houttuynia Cordata,” KR Patent No. 2002028976, 2002.
[152]	T. H. Hoang, Q. H. Tran, V. B. Ha and D. T. Nguyen, “Study on the Flavonoid Component Extracted from Leaves of Houttuynia cordata Thunb in Vietnam,” Tap Chi Duoc Hoc, No. 9, 2002, pp. 13-15.
[153]	H. Wagner, R. Bauer, P. Xiao, J. Chen and A. Probstle, “Herba Houttuyniae cordatae,” Chinese Drug Monographs and Analysis, Vol. 1, No. 6, 1997, pp. 1-11.
[154]	A. Proebstle, A. Neszmelyi, G. Jerkovich, H. Wagner and R. Bauer, “Novel Pyridine and 1,4-Dihydropyridine Alkaloids from Houttuynia cordata,” Natural Product Letters, Vol. 4, No. 3, 1994, pp. 235-240.