Optimum Dark Adaptation Period for Evaluating the Maximum Quantum Efficiency of Photosystem II in Ozone-Exposed Rice Leaves

Hiroki Hiroki Kobayakawa, Katsu Imai

School of Agriculture, Meiji University, Kawasaki, Japan..

DOI: 10.4236/ajps.2013.49215                

Abstract

Because the transient O₃ injury of leaves is lost with time, the evaluation of O₃ effect on the maximum quantum efficiency of PSII (F_v/F_m) is difficult. Thus, the authors examined F_v/F_m in rice leaves exposed to different O₃ concentrations (0, 0.1, and 0.3 cm³·m^-3, expressed as O⁰, O^0.1, and O^0.3) under different dark adaptation periods (0, 1, 5, 10, 20, and 30 min, expressed as D⁰, D¹, D⁵, D¹⁰, D²⁰, and D³⁰) to ascertain its optimum time span. F_v/F_m was inhibited by O₃; however in the O⁰ and O^0.1 plants, it recovered during dark adaptation. In the O^0.3 plants, F_v/F_m decreased gradually with time. F₀ was found to be increased by O₃, and it increased further in the O^0.3 plants during dark adaptation. Under a high light intensity, F_m was decreased by O₃, and the O₃-induced damage to F_v/F_m was therefore more pronounced. However, the sensitivity of F

Keywords

Dark-Adapted State; Oryza sativa; Ozone Stress; Photosystem II; Quantum Efficiency

Conflicts of Interest

The authors declare no conflicts of interest.

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