A Simple and Efficient Protocol for the Mass Propagation of Vanilla planifolia

Ab Rahman Zuraida, Kamarulzaman Hassan Fatin Liyana Izzati, Othman Ayu Nazreena, Wan Sembok Wan Zaliha, Che Mohd Zain Che Radziah, Zainal Zamri, Subramaniam Sreeramanan

Biotechnology Research Centre, MARDI Headquarters, MARDI HQ, Persiaran MARDI-UPM, Serdang Selangor, Malaysia;.
Department of Agrotechnology, Faculty of Agrotechnology and Food Science, Universiti Malaysia Terengganu, Kuala Terengganu, Malaysia;.
Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, Malaysia;.
School of Biological Sciences, Universiti Sains Malaysia (USM), Minden Heights, Malaysia..

DOI: 10.4236/ajps.2013.49205                

Abstract

The present study describes a direct shoot regeneration-based micropropagation procedure for Vanilla planifolia. Two types of explant (i.e. shoot apex and stem nodal segment) were screened for their shoot induction potential following a three-month treatment with 6-benzylaminopurine (BAP) and α-Naphthaleneacetic acid (NAA). Results indicated that the shoot apices were poor candidates for shoot induction whereas the stem nodal segments showed potential for shoot initiation at a rate of up to 6 shoots/explant. Stem nodal segments were the most responsive as shoots formed (55 shoots) directly following treatments with 1 mg/L BAP at half strength MS medium after the third subculture. In addition, more shoots were produced on solid medium treatments compared to the liquid medium treatments in two strengths of mediums tested. Regenerated plantlets derived from the 1 mg/L of BAP treatment were induced to root following a one month culture in growth regulator-free MS medium. There was 90% survival rate of the rooted plantlets after acclimatization in the greenhouse. The findings in the present study would be helpful for large-scale mass propagation of Vanilla planifolia using this simple and efficient protocol.

Keywords

Vanilla planifolia; Mass Propagation; Growth Regulator; In Vitro; BAP; NAA; IBA

Conflicts of Interest

The authors declare no conflicts of interest.

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