Antioxidant Activity and Phytochemical Screening of Crude Endophytes Extracts of Tabebuia argentea Bur. & K. Sch.

M. Govindappa, R. Channabasava, K. R. Sunil Kumar, K. C. Pushpalatha

Department of Biotechnology, Shridevi Institute of Engineering & Technology, Tumkur, India;.
Department of Studies in Biochem- istry, Mangalore University, PG Center, Cauvery Campus, Madikeri, India..

DOI: 10.4236/ajps.2013.48198                

Abstract

The aim of this study is to investigate in vitro antioxidant activities and the phytochemical screening endophytes. Seven different endophytic fungi were isolated from different parts of the plant and their extracts subjected to know antioxidant properties and phytochemical screening. Phytochemical analysis revealed the presence of tannins, flavonoids, steroids, alkaloids, phenols and proteins from different solvents extracts of different endophytes. The antioxidant activity was evaluated by six separated methods: scavenging of free radical DPPH, FRAP, TBA, superoxide radical, FTC and iron methods. All seven different endophytes yielded almost all phytochemicals in methanol extracts which were tested. The endophytes A. niger, Penicillium sp. and Trichoderma sp. have shown potential in vitro antioxidant activities. Further work is needful to isolate the exact compound which is responsible for antioxidant activity and biophysical characterization will be done in the future.

Keywords

Tabebuia argentea; Endophytes; Antioxidant Activity; Phytochemicals

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	Y. Shen, C. Chen and Y. Kou, “New Sewquiterpenehydroquinones from a Taiwanese Marine Songe, Hippospongiametachromia,” Journal of Natural Products, Vol. 64, No. 6, 2002, pp. 801-803. doi:10.1021/np000610c
[2]	W. Hills, “Heartwood and Tree Exudates,” Springer Series in Wood Science, Syracuse, New York, 1987, p. 267.
[3]	S. M. Wuerzberger, J. J. S. M. Pink, Planch, K. L. Byers, W. G. Bornmann and D. A. Boothman, “Induction of Apoptosis in MCF-7: WS8 Breast Cancer Cells by 3-Lapachone1,” Cancer Research, Vol. 58, 1998, pp. 1876-1885.
[4]	M. T. Murray and J. E. Pizzorno, “Encyclopedia of Natural Medicine,” 2nd Edition, PA4 Rocklin, Prima Pub, 1998, pp. 967-972.
[5]	I. I. Balassiano, S. A. Paulo, N. H. Silva, M. C. Cabral and M. C. Carvalho, “Demonstration of Lapachol as a Potential Drug for Fighting Cancer Metastases,” Oncology Reports, Vol. 13, 2005, p. 329.
[6]	M. N. Da Silva, M. C. B. V. Da Souza, V. F. Ferreira, A. V. Pinto, M. C. R. F. Pinto, M. S. V. Solange, S. M. S. V. Wardell and J. L. Wardell, “Synthesis of New Aldehyde Derivatives from β-Lapachone and Nor-β-Lapachone,” Arkivoc, 2003, pp. 156-168. doi:10.3998/ark.5550190.0004.a16
[7]	J. Breger, B. B. Fuchs, G. Aperis, T. I. Moy, F. M. Ausubel and E. Mylonakis, “Antifungal Chemical Compounds Identified Using a C. elegans Pathogenicity Assay,” PLoS Pathogens, Vol. 3, No. 2, 2007, pp. 168-178. doi:10.1371/journal.ppat.0030018
[8]	E. R. Almeida, “Anti-Inflammatory Action of Lapachol,” Journal of Ethnopharmacology, Vol. 29, 1990, pp. 239-241. doi:10.1016/0378-8741(90)90061-W
[9]	M. J. Teixeira, Y. M. Almeida, J. R. Viana, J. G. HolandaFilha, T. P. Rodrigues, J. R. C. Prata, I. C. B. Coelho, V. S. Rao and M. M. L. Pompeu, “In Vitro and in Vivo Leishmanicidal Activity of 2-Hydroxy-3-(3-Methyl-2-Butenyl)-1,4-Naphthoquinone (Lapachol),” Phytotherapy Research, Vol. 15, 2001, pp. 44-48. doi:10.1002/1099-1573(200102)15:1<44::AID-PTR685>3.0.CO;2-1
[10]	T. M. S. Silva, C. A. Camara, T. P. Barbosa, A. Z. Soares, L. C. Cunha, A. C. Pinto and M. D. Vargas, “Design, Synthesis and Antifungal Activity of a Novel Water Soluble Prodrug of Antifungal Triazole,” Bioorganic & Medicinal Chemistry Letters, Vol. 13, 2005, pp. 193-196. doi:10.1016/j.bmc.2004.09.043
[11]	T. Finkel and N. J. Holbrook, “Oxidants, Oxidative Stress and the Biology of Ageing,” Nature, Vol. 408, No. 6809, 2000, pp. 239-247. doi:10.1038/35041687
[12]	L. S. Cozma, “The Role of Antioxidant Therapy in Cardiovascular Disease,” Current Opinion in Lipidology, Vol. 15, No. 3, 2004, pp. 369-371. doi:10.1097/00041433-200406000-00020
[13]	R. W. Owen, A. Giacosa, W. E. Hull, R. Haubner, B. Spiegelhalder and H. Bartsch, “The Antioxidant/Anticancer Potential of Phenolic Compounds Isolated from Olive Oil,” European Journal of Cancer, Vol. 36, No. 10, 2000, pp. 1235-1247. doi:10.1016/S0959-8049(00)00103-9
[14]	T. S. Sadananda, R. Nirupama, K. Chaithra, M. Govindappa, C. P. Chandrappa and B. VinayRaghavendra, “Antimicrobial and Antioxidant Activities of Endophytes from Tabebuia argentea and Identification of Anticancer Agent (Lapachol),” Journal of Medicinal Plants Research, Vol. 5, No. 16, 2011, pp. 3643-3652.
[15]	T. Theantana, K. D. Hyde and S. Lumyong, “Asparginase Production by Endophytic Fungi from Thai Medicinal Plants: Cytotoxic Properties,” International Journal of Integrative Biology, Vol. 7, No. 1, 2009, pp. 1-8.
[16]	M. B. Ellis, “Dematiaceoushypomycetes,” Common Wealth Mycological Institute, Kew Surrey, 1971.
[17]	H. L. Barnett and B. B. Hunter, “Illustrated Genera of Imperfect Fungi,” 2nd Edition, Burgess Publishing Company, Minnesota, 1972.
[18]	A. L. Bandoni, M. E. Mendiondo, R. V. D. Rondina and J. D. Coussio, “Survey of Argentine Medicinal Plants, Folklore and Phytochemical Screening,” Economic Botany, Vol. 30, No. 2, 1976, pp. 161-185. doi:10.1007/BF02862962
[19]	R. H. Thomson, “Naturally Occurring Quinones,” Recent Advances, Chapman and Hall, London, Vol. 142, 1987, pp. 144-147.
[20]	A. Tomic, S. Petrovic, M. Pavlovic, B. Trajkovski, M. Milenkovic, D. Vucicevic and M. Niketic, “Antimicrobial and Antioxidant Properties of Methanol Extracts of Two Athamantaturbish Subspecies,” Pharmaceutical Biology, Vol. 47, No. 4, 2009, pp. 314-319. doi:10.1080/13880200902748452
[21]	M. Gulluce, H. Ozer, O. Baris, D. Daferera, F. Sahin and M. Polissiou, Turkish Journal of Biology, Vol. 30, 2006, pp. 231-233.
[22]	C. C. Winterbourne, R. E. Hawkins, M. Brain and R. W. Carrel, “The Estimation of Red Cell Superoxide Dismutase Activity,” Journal of Laboratory and Clinical Medicine, Vol. 85, No. 2, 1975, pp. 337-341.
[23]	I. F. F. Benzie and J. J. Strain, “Ferric Reducing Ability of Plasma (FRAP) as a Measure of Antioxidant Power: The FRAP Assay,” Analytical Biochemistry, Vol. 239, No. 1, 1996, pp. 70-76. doi:10.1006/abio.1996.0292
[24]	D. Gülcin, R. Elias, A. Gepdiremen, K. Taoubi and E. Koksal, “Antioxidant Secoiridoids from Fringe Tree (Chionanthusvirginicus L.),” Wood Science and Technology, Vol. 43, No. 3-4, 2009, pp. 195-212. doi:10.1007/s00226-008-0234-1
[25]	M. Elmastas, O. Isildak, I. Turkekul and N. Temur, “Determination of Antioxidant Activity and Antioxidant Compounds in Wild Edible Mushrooms,” Journal of Food Composition and Analysis, Vol. 20, No. 3, 2007, pp. 337-345. doi:10.1016/j.jfca.2006.07.003
[26]	K. Saha, N. H. Lajis, D. A. Israf, A. S. Hamzah, S. Khozirah, S. Khamis and A. Syahida, “Evaluation of Antioxidant and Nitric Oxide Inhibitory Activities of Selected Malaysian Medicinal Plants,” Journal of Ethnopharmacology, Vol. 92, No. 2-3, 2004, pp. 263-267. doi:10.1016/j.jep.2004.03.007
[27]	A. Jain, M. Soni, L. Deb, A. Jain, S. Rout, V. Gupta and K. Krishna, “Antioxidant and Hepatoprotective Activity of Ethanolic and Aqueous Extracts of Momordicadioica Roxb. Leaves,” Journal of Ethnopharmacology, Vol. 115, No. 1, 2008, pp. 61-66. doi:10.1016/j.jep.2007.09.009
[28]	K. Srinivasan, L. K. Jagadish, R. Shenbhagaraman and J. Muthumary, “Antioxidant Activity of Endophytic Fungus Phyllosticta sp. Isolated from Guazumatomentosa,” Journal of Phytology, Vol. 2, No. 6, 2010, pp. 37-41.
[29]	L. Barros, S. Oliveira, A. M. Carvalho and I. C. F. R. Ferreira, “In Vitro Antioxidant Properties and Characterization in Nutrients and Phytochemicals of Six Medicinal Plants from the Portuguese Folk Medicine,” Industrial Crops and Products, Vol. 32, No. 3, 2010, pp. 572-579. doi:10.1016/j.indcrop.2010.07.012
[30]	S. Phongpaichit, N. Rungjindamai, N. Rukachaisirikul and J. Sakayaroj, “Antimicrobial Activity in Cultures of Endophytic Fungi Isolated from Garcinia Species,” FEMS Immunology & Medical Microbiology, Vol. 48, No. 3, 2006, pp. 367-372. doi:10.1111/j.1574-695X.2006.00155.x
[31]	L. K. Jagadish, R. Shenbhagaraman, V. V. Krishnan and V. Kaviyarasan, “Studies on the Phytochemical, Antioxidant and Antimicrobial Properties of Three Pleurotus Species Collected Indigenously,” Journal of Molecular Biology and Biotechnology, Vol. 1, 2008, pp. 20-29.
[32]	A. A. Adedapo, F. O. Jimoh, S. Koduru, A. J. Afolayan and P. J. Masika, “Antibacterial and Antioxidant Properties of the Methanol Extracts of the Leaves and Stems of Calpurnia aurea,” BMC Complementary and Alternative Medicine, Vol. 8, No. 1, 2008, pp. 53-58.
[33]	Y. N. V. Maslarova, “Inhibiting Oxidation,” In: J. Pokorny, N. Yanishlieva and M. H. Gordon, Eds., Antioxidants in Food: Practical Applications, CRC Press, Woodhead Publishing Limited, Cambridge, 2001, pp. 22-70. doi:10.1533/9781855736160.1.22
[34]	H. Y. Lai, Y. Y. Yau and K. H. Kim, “Blechnumorientale Linn—A Fern with Potential as Antioxidant, Anticancer and Antibacterial Agent,” BMC Complementary and Alternative Medicine, Vol. 10, No. 1, 2010, pp. 15-18. doi:10.1186/1472-6882-10-15
[35]	J. Grassmann, “Terpenoids as Plant Antioxidants,” Vitamins & Hormones, Vol. 72, 2005, pp. 505-535. doi:10.1016/S0083-6729(05)72015-X
[36]	I. Gulcin, V. Mshvildadze, A. Gepdiremen and R. Elias, “Antioxidant Activity of Saponins Isolated from Ivy: Alpha-Hederin, Hederasaponin-C, Hederacolchiside-E and Hederacolchiside-F,” Planta Medica, Vol. 70, No. 6, 2004, pp. 561-563. doi:10.1055/s-2004-827158
[37]	S. K. Gond, V. C. Verma, A. Kumar, V. Kumar and R. N. Kharwar, “Study of Endophytic Fungal Community from Different Parts of Aegle marmelos Correae (Rutaceae) from Varanasi (India),” World Journal of Microbiology and Biotechnology, Vol. 23, No. 10, 2007, pp. 1371-1375. doi:10.1007/s11274-007-9375-x
[38]	S. H. Faeth and K. E. Hammon, “Fungal Endophytes in Oak Tree; Long Term Pattern of Abundance and Association with Leaf Miners,” Ecology, Vol. 78, No. 3, 1997, pp. 810-819. doi:10.1890/0012-9658(1997)078[0810:FEIOTL]2.0.CO;2
[39]	V. C. Verma, S. K. Gond, A. Kumar, A. Mishra, R. N. Kharwar and A. C. Gange, “Endophyticactinomycetes from Azadirachtaindica A. Juss.: Isolation, Diversity and Anti-Microbial Activity,” Microbial Ecology, Vol. 57, No. 4, 2009, pp. 749-756. doi:10.1007/s00248-008-9450-3
[40]	G. Al-Naqeeb, M. S. Ismail and A. Al-Zuba, “Fatty Acid Profile, α-Tocopherol Content and Total Atioxidant Activity of Oil Extracted from Nigella sativaseeds,” International Journal of Pharmacology, Vol. 5, No. 4, 2009, pp. 244-250. doi:10.3923/ijp.2009.244.250
[41]	X. Liu, M. Dong, X. Chen, M. Jiang, X. Lv and G. Yan, “Antioxidant Activity and Phenolics of an Endophytic xylaria sp. from Gingko biloba,” Food Chemistry, Vol. 105, No. 2, 2007, pp. 548-554. doi:10.1016/j.foodchem.2007.04.008