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Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis

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DOI: 10.4236/abb.2010.15055    10,852 Downloads   26,348 Views   Citations

ABSTRACT

The Acetobacter estunensis Rep34 protein participates in the replication of bacterial plasmid pGP2. The Rep34 protein of the A. estunensis, was cloned to the expression vector, that ensure fusion with a His-tag sequence (Rep34 His-tagged), over-expressed in Escherichia coli and purified by metal-affinity chromatography to yield a highly purified and active protein. On this purified protein number different activities and motifs were detected. DNA band-shift assays showed that the Rep34 His-tagged protein bound to the regulation region for replication on the linear double-stranded DNA. In the protein was determined phosphatase activity, ATPase activity and protein is possible to unwind double strand DNA.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Grones, P. and Grones, J. (2010) Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis. Advances in Bioscience and Biotechnology, 1, 417-425. doi: 10.4236/abb.2010.15055.

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