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Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol

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DOI: 10.4236/jbnb.2013.43030    2,646 Downloads   3,741 Views   Citations

ABSTRACT

Model test-systems for biochemical determination of glucose and cholesterol are designed by covalent coupling of enzymes to the surface of modified nanodiamonds (MND). In both cases after immobilization on MND the enzymes exhibit activity and catalyze the formation of a colored product in the chain of successive enzymatic reactions of oxidative azocoupling (Н2О2—4-aminoantipyrine—phenol). Produced MND-enzymes complexes: function in DI water and various buffers, over a wide range of temperature and pH, provide for the linear product yield over a broad concentration range of analyzed substances, make possible to test cholesterol and glucose in vitro many times, exhibit functional activity after long storage at + 4°C.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

N. Ronzhin, A. Baron, E. Mamaeva, A. Puzyr and V. Bondar, "Nanodiamond-Based Tests Systems for Biochemical Determination of Glucose and Cholesterol," Journal of Biomaterials and Nanobiotechnology, Vol. 4 No. 3, 2013, pp. 242-246. doi: 10.4236/jbnb.2013.43030.

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