Determination of Tramadol in Human Serum by Capillary Electrophoresis with the End-Column Electrochemiluminescence Detection

Caiyun Zhang, Tao Liu, Xiaoli Zhang, Erbao Liu

The School of Chemistry and Materials Science, Shanxi Normal University, Linfen, China.

DOI: 10.4236/ojbiphy.2013.32015                

Abstract

A capillary electrophoresis (CE) coupled with end-column electrochemiluminescence (ECL) detection method for the analysis of tramadol (TMD) has been investigated. ECL detection was working electrode biased at 1.2 V in a 20mmol·L^-1 sodium phosphate buffer (pH = 8.0) containing 5 mmol·L^-1 Ru (where bpy = 2,2’-bipyridyl). Linear correlation (r ≥ 0.997) between ECL intensity and drug concentration was obtained in the range 3 × 10^-4 - 6 × 10^-6 mol·L^-1. The limits of detection (LODs) for tramadol in water was 3.012 × 10^-8 mol·L^-1(S/N = 3). The relative standard deviation values on peak size (10^-5 mol·L^-1 level) and migration time for the tramadol were 4.58% and 1.39% (n = 10), respectively. Applicability of the CE-ECL method to the analysis of human serum spiked with tramadol was examined

Keywords

Capillary Electrophoresis; Electrochemiluminescence; Tramadol Hydrochloride; Human Serum

Conflicts of Interest

The authors declare no conflicts of interest.

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