Analysis of bacterial growth at various distances from an antimicrobial surface

Abstract

Antimicrobial materials have been used in various environments. However, the activity of cells at a distance from the antimicrobial materials has not been elucidated. In the present study, the cell growth of Escherichia coli NBRC 3972 was observed at different distances (0 - 300 μm) from the antimicrobial surface under various nutrient concentrations, from full strength nutrient broth (NB) to 1/40 NB. Under higher nutrient concentrations, NB and 1/2 NB, no antimicrobial effect on cell growth was observed at any distance from the surface. Under lower nutrient concentrations, 1/10 NB and 1/40 NB, the growth of cells directly contacting the antimicrobial surface (at 0 μm from the surface) was blocked immediately after inoculation on the surface. However, at distances of 100 - 300 μm from the surface, the cells grew normally for a while, and then stopped the growth; earlier growth discontinuation was observed for cells closer to the surface. It was suggested that the antimicrobial agent (silver ions) is released from the antimicrobial surface into the medium and that the diffusion of the silver ions may influence the lag in the antimicrobial effects observed at distances away from the antimicrobial surface. The present study reveals the possibility that antimicrobial activity in the environments where the antimicrobial material is used depends on the distance from the surface and the surrounding nutrient concentrations.

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Nunose, S. , Iwai, R. , Okuda, S. , Tsuchiya, Y. and Morisaki, H. (2013) Analysis of bacterial growth at various distances from an antimicrobial surface. Advances in Bioscience and Biotechnology, 4, 563-569. doi: 10.4236/abb.2013.44074.

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1] Casey, A.L., Adams, D., Karpanen, T.J., Lambert, P.A., Cookson, B.D., Nightingale, P., Miruszenko, L., Shillam, R., Christian, P. and Elliott, T.S. (2009) Role of copper in reducing hospital environment contamination. Journal of Hospital Infection, 74, 72-77. doi:10.1016/j.jhin.2009.08.018
[2] Galeano, B., Korff, E. and Nicholson, W.L. (2003) Inactivation of vegetative cells, but not spores, of Bacillus anthracis, B. cereus, and B. subtilis on stainless steel surfaces coated with an antimicrobial silverand zinc-containing zeolite formulation. Applied Environmental Microbiology, 69, 4329-4331. doi:10.1128/AEM.69.7.4329-4331.2003
[3] Leaper, D.J. (2006) Silver dressings: Their role in wound management. International Wound Journal, 3, 282-294. doi:10.1111/j.1742-481X.2006.00265.x
[4] Lellouche, J., Kahana, E., Elias, S., Gedanken, A. and Banin, E. (2009) Antibiofilm activity of nanosized magnesium fluoride. Biomaterials, 30, 5969-5978. doi:10.2147/IJN.S26770
[5] Monteiro, D.R., Gorup, L.F., Takamiya, A.S., Ruvollo-Filho, A.C., de Camargo, E.R. and Barbosa, D.B. (2009) The growing importance of materials that prevent microbial adhesion: Antimicrobial effect of medical devices containing silver. International Journal of Antimicrobial Agents, 34, 103-110. doi:10.1080/08927014.2011.599101
[6] Stevens, K.N., Crespo-Biel, O., van den Bosch, E.E., Dias, A.A., Knetsch, M.L., Aldenhoff, Y.B., van der Veen, F.H., Maessen, J.G., Stobberingh, E.E. and Koole, L.H. (2009) The relationship between the antimicrobial effect of catheter coatings containing silver nanoparticles and the coagulation of contacting blood. Biomaterials, 30, 36823690. doi:10.1016/j.biomaterials.2009.03.054
[7] Engelsman, A.F., van der Mei, H.C., Busscher, H.J. and Ploeg, R.J. (2008) Morphological aspects of surgical meshes as a risk factor for bacterial colonization. British Journal of Surgery, 95, 1051-1059. doi:10.1002/bjs.6154
[8] Neut, D., Hendriks, J.G., van Horn, J.R., van der Mei, H.C. and Busscher, H.J. (2005) Pseudomonas aeruginosa biofilm formation and slime excretion on antibioticloaded bone cement. Acta Orthopaedica, 76, 109-114. doi:10.1080/00016470510030427
[9] Percival, S.L., Bowler, P.G. and Russell, D. (2005) Bacterial resistance to silver in wound care. Journal of Hospital Infection, 60, 1-7. doi:10.1016/j.jhin.2004.11.014
[10] Silver, S., Gupta, A., Matsui, K. and Lo, J.F. (1999) Resistance to Ag(I) cations in bacteria: Environments, genes and proteins. Metal-Based Drugs, 6, 315-320. doi:10.1155/MBD.1999.315
[11] Japanese Industrial Standard Committee (2000) Antimicrobial products-test for antimicrobial activity and efficacy. Publication JIS Z 2801:2000. Japanese Standards Association, Tokyo.
[12] Yamada, H., Takahashi, N., Okuda, S., Tsuchiya Y. and Morisaki, H. (2010) Direct observation and analysis of bacterial growth on an antimicrobial surface. Applied Environmental Microbiology, 76, 5409-5414. doi:10.1128/AEM.00576-10
[13] Tsuchiya, Y., Ikenaga, M., Kuriawan, A., Hiraki, A., Arakawa, T., Kusakabe, R. and Morisaki, H. (2009) Nutrientrich microhabitats within biofilms are synchronized with the external environment. Microbes Environment, 24, 4351. doi:10.1264/jsme2.ME08547
[14] Stewart, P.S., Murga, R., Srinivasani, R. and de Beer, D. (1995) Biofilm structural heterogeneity visualized by three microscopic methods. Water Research, 29, 2006-2009. doi:10.1016/0043-1354(94)00339-9
[15] Gunsalus, I.C. and Hand, D.B. (1941) The use of bacteria in the chemical determination of total vitamin c. Journal of Biological Chemistry, 141, 853-858.
[16] Tsubai, Y. (1997) Mechanism of exerting antimicrobial activity of inorganic antimicrobial materials. In: Oya, A., Ed., Diversifying Inorganic Antimicrobial Materials and Their Application, Industrial Publishing and Consulting, Tokyo, 61.

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