An in vitro study of antimicrobial agents incorporated into interim restorative materials

Arely Denisse Garza Garza, Violet I. Haraszthy, Jane D. Brewer, Edward Monaco, Jennifer Kuracina, Joseph J. Zambon

Department of Periodontics and Endodontics, University at Buffalo, School of Dental Medicine, Buffalo, USA.
Department of Restorative Dentistry, University at Buffalo, School of Dental Medicine, Buffalo, USA.

DOI: 10.4236/ojst.2013.31017                

Abstract

Aim: To test the hypothesis that antimicrobial agents can be incorporated into bis-acryl resin and that the material will inhibit bacterial growth in vitro. Materials and Methods: 0.5%, 1% and 2% triclosan (5-chloro-2-(2,4-dichlorophenoxy)phenol) and 0.05% 0.01% and 0.15% cetylpyridinium chloride were incorporated into bis-acryl resins and molded into 1 mm × 10 mm discs. The discs were placed on bacterial culture media inoculated with Aggregatibacter actinomycetemcomitans strain Y4, Porphyromonas gingivalis strain SUNYaB A7A1-28 or Streptococcus gordonii strain 10558, incubated and examined for growth inhibition. Results: Triclosan-containing bisacryl resin discs produced dose-dependent zones of growth inhibition for A. actinomycetemcomitans but not S. gordonii or P. gingivalis. Cetylpyridinium chloride-containing discs produced dose-dependent zones of growth inhibition for Porphyromonas gingivalis and Streptococcus gordonii but not A. actinomycetemcomitans. Conclusion: Triclosan and cetylpyridinium chloride can be incorporated into bis-acryl resin and can inhibit the growth of plaque bacteria in a dose dependent manner. Bis-acryl resin containing antimicrobial agents may inhibit in vivo plaque accumulation and gingival inflammation.

Keywords

Interim Restorations; Bis-Acryl Resin; Triclosan; Cetylpyridinium Chloride

Conflicts of Interest

The authors declare no conflicts of interest.

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