Gold Nanoparticles: Acceptors for Efficient Energy Transfer from the Photoexcited Fluorophores

Debanjana Ghosh, Nitin Chattopadhyay

Department of Chemistry, Jadavpur University, Kolkata, India.

DOI: 10.4236/opj.2013.31004                

Abstract

The citrate reduction method of synthesis of gold nanoparticles (AuNP) is standardized with the assistance of instruments like spectrophotometer and TEM. A correlation has been developed between the particle diameter and the fractional concentration of the reductant. This enables one to assess the diameter of the AuNP to be synthesized, in advance, from the composition of the reaction mixture and the diameter of the synthesized particles can be confirmed simply from spectrophotometry. Further, it has been demonstrated that the synthesized AuNPs serve as excellent acceptors for a super-efficient energy transfer (ET) from the donor coumarin 153, leading to a quenching of fluorescence of the latter. The Stern-Volmer constants determined from the fluorescence lifetimes are in the range 10⁷ - 10⁹ mol^-1·dm³ and are orders of magnitude higher than the normal photochemical quenching processes. The energy transfer efficiency increases radically with an increase in the size of the metal nanoparticle. The highly efficient energy transfer and the variation of the efficiency of the ET process with a variation of the particle size is ascribed to a large enhancement in the extinction coefficient and an increase in the spectral overlap between the plasmon absorption band of AuNPs and the fluorescence spectrum of C153 with an increase in the size of the nanoparticles. The impact of the work remains in providing a demonstration of a super quenching effect of the AuNPs and projects that they can be exploited for developing biosensors with high degree of sensitivity, if tagged to the biomacromolecules.

Keywords

Gold Nanoparticles; TEM; Spectrophotometry; Fluorescence Quenching; Energy Transfer; Plasmon Absorption; Super Quenching

Conflicts of Interest

The authors declare no conflicts of interest.

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