Cadmium Accumulation in Marsilea minuta Linn. and Its Antioxidative Responses

Kingsuk Das, Chiranjib Mandal, Nirmalya Ghosh, Narottam Dey, Malay Kumar Adak

Department of Biotechnology, Visva Bharati Visvavidyalaya, Santiniketan, India.
Plant Physiology and Plant Molecular Biology Research Unit, Department of Botany, University of Kalyani, Kalyani, India.

DOI: 10.4236/ajps.2013.42A048                

Abstract

The present study was carried out to investigate the extent of cadmium (Cd) accumulation with its possible impact on physiological and biochemical basis of heavy metal tolerance in Marsilea minuta Linn. Cd salt (0 μM, 50 μM and 100 μM) was allowed to absorb by the plants for prolong days in hydroponic culture and a significant deterioration of the plant biomass was recorded. However, roots absorbed more metals than the leaves. Plants recorded a significant rise of superoxide (O^-₂) and hydrogen peroxide (H₂O₂). A noticeable amount of protein oxidation and lipid peroxidation were in proportionate to Cd accumulation. Anthocyanin and flavonoid content were decreased as compared to control condition. Superoxide dismutase (SOD), guaiacol peroxidase (GPX) and glutathione reductase (GR) contributed their antioxidative functions according to the Cd doses. The expression of GR was also evident from its activity staining in gel. So, it may suggest that antioxidative enzymes are up regulated and likely to be responsible for tolerance to Cd induced oxidative stress in Marsilea minuta Linn.

Keywords

Oxidative Stress; Heavy Metals; Aquatic Fern

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	R. W. Feng, C. Y. Wei, S. X. Tu and X. Sun, “Interactive Effects of Selenium and Arsenic on Their Uptake by Pteris vittata L. under Hydropronic Conditions,” Environmental and Experimental Botany, Vol. 65, No. 2-3, 2009, pp. 363-368. doi:10.1016/j.envexpbot.2008.11.013
[2]	B. Semane, A. Cuypers, K. Smeets, F. Van Belleghem, N. Horemans and H. Sehat “Cadmium Responses in Arabidopsis thaliana: Glutathione Metabolism and Antioxidative Defense System,” Physiologia Plantarum, Vol. 129, No. 3, 2007, pp. 519-528. doi:10.1111/j.1399-3054.2006.00822.x
[3]	S. S. Sharma and K. J. Dietz, “The Relationship between Metal Toxicity and Cellular Redox Imbalance,” Trends in Plant Science, Vol. 14, No. 1, 2009, pp. 43-50. doi:10.1016/j.tplants.2008.10.007
[4]	M. Pielichowska and M. Wierzbicka, “Uptake and Localization of Cadmium by Biscutella laevigata, a Cadmium Hyperaccumulator,” Acta Biologica Cracoviensia Series Botanica, Vol. 46, No. 4, 2004, pp. 57-63.
[5]	R. A. Root, R. J. Miller and D. E. Koeppe, “Uptake of Cadmium-Its Toxicity, and Effect on the Iron Ratio in Hydroponically Grown Corn,” Journal of Environmental Quality, Vol. 4, No. 4, 1975, pp. 473-476.
[6]	A. Roychoudhury, S. Basu and D. N. Sengupta, “Antioxidants and Stress-Related Metabolites in the Seedlings of Two Indica Rice Varieties Exposed to Cadmium Chloride Toxicity,” Acta Physiologiae Plantarum, Vol. 34, No. 3, 2012, pp. 835-847. doi:10.1007/s11738-011-0881-y
[7]	P. J. Sobrino, V. C. Ortega, C. M. L. Flores, C. Escobar, C. F. F. Del and L. E. Hernández, “Differential Alterations of Antioxidant Defenses as Bioindicators of Mercury and Cadmium Toxicity in Alfalfa,” Chemosphere, Vol. 77, No. 7, 2009, pp. 946-954. doi:10.1016/j.chemosphere.2009.08.007
[8]	C. Fusco, F. Zaina, S. Atanasio, M. Romano, A. Negrini and S. Negrini, “Physical Exercises in the Treatment of Adolescent Idiopathic Scoliosis: An Updated Systematic Review,” Physiotherapy Theory and Practice, Vol. 27, No. 1, 2011, pp. 80-114. doi:10.3109/09593985.2010.533342
[9]	M. Beladi, A. Kashani, D. Habibi, F. Paknajad and M. Golshan, “Uptake and Effects of Lead and Copper on Three Plant Species in Contaminated Soils,” African Journal of Agricultural Research, Vol. 6, No. 15, 2011, pp. 3483-3492.
[10]	R. W. Feng and C. Y. Wei, “Antioxidation Mechanisms on Selenium Accumulation in Pteris vittata L., Potential Selenium Phytoremediation Plant,” Plant Soil and Environment, Vol. 58, No. 3, 2012, pp. 105-110.
[11]	L. Q. Ma, K. M. Komar, C. Tu, W. H. Zhang, Y. Cai and E. D. Kennelley, “A Fern That Hyperaccumulates Arsenic,” Nature, Vol. 409, No. 6820, 2001, p. 579. doi:10.1038/35054664
[12]	R. Singh, D. P. Singh, N. Kumar, S. K. Bhargava and S. C. Barman, “Accumulation and Translocation of Heavy Metals in Soil and Plants from Fly Ash Contaminated Area,” Journal of Environmental Biology, Vol. 31, No. 4, 2010, pp. 421-430.
[13]	Z. E. Hawa, H. I. Jaafar and E. K. Mohd, “Phenolics and Flavonoid Compounds, Phenylalanine Ammonia Lyase and Antioxidant Activity Responses to Elevated CO2 in Labisia pumila (Myrisinaceae),” Molecules, Vol. 17, No. 6, 2012, pp. 6331-6347. doi:10.3390/molecules17066331
[14]	P. Satapathy, V. M. M. Achary and B. B. Brahma, “Aluminium-Induced Abiotic Stress Counteracts Fusarium Infection in Cajanas cajan (L.) Millsp,” Journal of Plant Interactions, Vol. 7, No. 2, 2012, pp. 121-128. doi:10.1080/17429145.2011.584133
[15]	N. Ghosh, M. K. Adak, P. D. Ghosh, S. Gupta, D. N. Sengupta and C. Mandal, “Differential Responses of Two Rice Varieties to Salt Stress,” Plant Biotechnology Reports, Vol. 5, No. 1, 2011, pp. 89-103. doi:10.1007/s11816-010-0163-y
[16]	R. L. Heath and L. Packer, “Photoreoxidation in a Isolated Chloroplasts I. Kinetics & Stoichiometry of Fatty Acid Peroxidation,” Archives in Biochemistry and Biophysics, Vol. 125, No. 1, 1968, pp. 189-198. doi:10.1016/0003-9861(68)90654-1
[17]	A. Roychoudhury, S. Basu and D. N. Sengupta, “Antioxidants and Stress Related Metabolits in the Seedlings of Two Indica Rice Varieties Exposed to Cadmium Chloride Toxicity,” Acta Physiologia Plantarum, Vol. 34, No. 3, 2012, pp. 835-847. doi:10.1007/s11738-011-0881-y
[18]	A. Kiba, C. Miyake, K. Toyoda, Y. Ichinose, T. Yamada and T. Shairaishi, “Superoxide Generation in Extracts from Isolated Plant Cell Walls Is Regulated by Fungal Signal Molecules,” Phytopathology, Vol. 87, No. 8, 1997, pp. 846-852. doi:10.1094/PHYTO.1997.87.8.846
[19]	A. Ishida, K. Ookubo and K. Ono, “Formation of Hydrogen Peroxide by NAD(P)H Oxidation with Isolated Cell Wall Associated Peroxidase from Cultured Liverwort Cells. Marchantia polymorpha L.,” Plant Cell Physiology, Vol. 28, No. 4, 1987, pp. 723-726.
[20]	A. Roychoudhury, C. Roy and D. N. Sengupta, “Transgenic Tobacco Plant Overexpressing the Heterologous Lea Gene Rab 16A from Rice during High Salt and Water Deficit Display Enhanced Salinity Stress,” Plant Cell Reports, Vol. 26, No. 10, 2007, pp. 1839-1859. doi:10.1007/s00299-007-0371-2
[21]	S. Basu, A. Roychoudhury, P. P. Saha and D. N. Sengupta, “Comparative Analysis of Some Biochemical Responses of Three Indica Rice Varieties during Polyethylene Glycol-Mediated Water Stress Exhibits Distinct Varietal Differences,” Acta Physiologia Plantarum, Vol. 32, No. 3, 2010, pp. 551-563. doi:10.1007/s11738-009-0432-y
[22]	O. Borsani, P. Diaz, M. F. Agius, V. Valpuesta and J. Monza, “Water Stress Generates an Oxidative Stress through the Induction of a Specific Cu/Zn Superoxide Dismutase in Loyus corniculatus Leaves,” Plant Science, Vol. 161, No. 4, 2001, pp. 757-763. doi:10.1016/S0168-9452(01)00467-8
[23]	A. Drotar, P. Phelps and R. Fall, “Evidence for Glutathione Peroxidase Activities in Cultured Plant Cells,” Plant Science, Vol. 42, No. 1, 1985, pp. 35-40. doi:10.1016/0168-9452(85)90025-1
[24]	I. Cakmak, D. Strbac and H. Marschner, “Activities of Hydrogen Peroxide-Scavenging Enzymes in Germinating Wheat Seeds,” Journal of Experimental Botany, Vol. 44, No. 258, 1993, pp. 127-132. doi:10.1093/jxb/44.1.127
[25]	C. Mandal, N. Ghosh, S. Maiti, K. Das, S. Gupta, N. Dey and M. K. Adak, “Antioxidative Responses of Salvinia (Salvinia natans Linn.) to Aluminium Stress and It’s Modulation by Polyamine,” Physiology and Molecular Biology of Plants, Vol. 19, No. 1, 2013, pp. 91-103. doi:10.1007/s12298-012-0144-4
[26]	X. D. Cao, L. Q. Ma and C. Tu, “Antioxidative Response to Arsenic in the Arsenic Hyperaccumulation Chinese Brake fern (Pteris vittata L.),” Environmental Pollution, Vol. 128, No. 3, 2004, pp. 317-325. doi:10.1016/j.envpol.2003.09.018
[27]	S. Kochhar and V. K. Kochhar, “Expression of Antioxidant Enzymes and Heat Shock Proteins in Relation to Combined Stress Cadmium and Heat in Vigna mungo Seedlings,” Plant Science, Vol. 168, No. 4, 2005, pp. 921-929. doi:10.1016/j.plantsci.2004.11.013
[28]	M. Srivastava, L. Q. Ma and J. A. Cotruvo, “Uptake and Distribution of Selenium in Different Fern Species,” International Journal of Phytoremediation, Vol. 7, No. 1, 2005, pp. 33-42. doi:10.1080/16226510590915792