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Pectin shows antibacterial activity against Helicobacter pylori

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DOI: 10.4236/abb.2013.42A037    3,236 Downloads   6,396 Views   Citations

ABSTRACT

Pectins are the collective name for a group of heterogeneous, high molecular weight, branched polysaccharides that are found in the cell walls of higher plants. In this study, we intend to determine the antibacterial activity of pectin against reference strains and clinical isolates of Helicobacter pylori. The results show that pectin produced antibacterial effects on all the 16 clinical isolates and 2 reference strains of H. pylori with the greatest antibacterial effect at a low pH (5.0) versus higher pHs. The lowest Minimum Inhibitory Concentration recorded was of 0.016 μg/μl. Antibiotic resistance, therapy costs, and undesirable side effects stress the need for new antimicrobials or alternative therapies. The results from our study can further our goal of future eradication of H. pylori infection using new molecules.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Daoud, Z. , Sura, M. and Abdel-Massih, R. (2013) Pectin shows antibacterial activity against Helicobacter pylori. Advances in Bioscience and Biotechnology, 4, 273-277. doi: 10.4236/abb.2013.42A037.

References

[1] Carpita, N.C. and Gibeaut, D.M. (1993) Structural models of primary cell walls in flowering plants: Consistency of molecular structure with the physical properties of walls during growth. The Plant Journal, 3, 1-30. doi:10.1111/j.1365-313X.1993.tb00007.x
[2] Knox, J.P., Linstead, P.J., King, J., Cooper, C. and Roberts, K. (1990) Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta, 181, 512-521. doi:10.1007/BF00193004
[3] Behall, K. and Reiser, S. (1986) Effects of Pectin on Human Metabolism, Chemistry and Function of Pectins. American Chemical Society, Washington, 248-265.
[4] Heitman, D.W., Hardman, W.E. and Cameron, I.L. (1992) Dietary supplementation with pectin and guar gum on 1,2-dimethylhydrazine-induced colon carcinogenesis in rats. Carcinogenesis, 13, 815-818. doi:10.1093/carcin/13.5.815
[5] Brouns, F., Theuwissen, E., Adam, A., Bell, M., Berger, A. and Mensink, R.P. (2012) Cholesterol-lowering properties of different pectin types in mildly hyper-cholesterolemic men and women. European Journal of Clinical Nutrition, 66, 591-599. doi:10.1038/ejcn.2011.208
[6] Kenneth, E.L. and McColl, M.D. (2010) Helicobacter pylori Infection. New England Journal of Medicine, 362, 1597-1604.
[7] Pounder, R.E. (1995) The prevalence of Helicobacter pylori infection in different countries, Aliments pharmacology therapy. Department of Medicine, Division of Gastroenterology, Rhode Island Hospital and Brown University, Providence, 9, 33-39.
[8] El Nakeeb, M.A. and Youssef, R.T. (1970) Study of the antimicrobial action of pectin. II. Factors affecting the bactericidal activity of pectin. Planta Medica, 18, 295302. doi:10.1055/s-0028-1099782
[9] Rydning, A., Weberg, R., Lange, O. and Berstad, A. (1986) Healing of benign gastric ulcer with low-dose antacids and fiber diet. Gastroenterology, 91, 56-61.
[10] Setiawan, V.W., Zhang, Z.F., Yu, G.P., et al. (2001) Protective effect of green tea on the risks of chronic gastritis and stomach cancer. International Journal of Cancer, 92, 600-604. doi:10.1002/ijc.1231
[11] Lee, K.M., Yeo, M., Choue, J.S., et al. (2004) Protective mechanism of epigallocatechin-3-gallate against Helicobacter pylori-induced gastric epithelial cytotoxicity via the blockage of TLR-4 signaling. Helicobacter, 9, 632642. doi:10.1111/j.1083-4389.2004.00281.x
[12] Gao, C.M., Takezaki, T., Ding, J.H., Li, M.S. and Tajima, K. (1999) Protective effect of allium vegetables against both esophageal and stomach cancer: A simultaneous case-referent study of a high-epidemic area in Jiangsu Province, China. Japanese Journal of Cancer Research, 90, 614-621. doi:10.1111/j.1349-7006.1999.tb00791.x
[13] You, W.C., Blot, W.J., Chang, Y.S. et al. (1989) Allium vegetables and reduced risk of stomach cancer. Journal of the National Cancer Institute, 81, 162-164. doi:10.1093/jnci/81.2.162
[14] Brown, L.M., Thomas, T.L., Ma, J.L., Chang, Y.S., You, W.C., Liu, W.D., Zhang, L., Pee, D. and Gail, M.H. (2002) Helicobacter pylori infection in rural China: Demographic, lifestyle and environmental factor. International Journal of Epidemiology, 31, 638-645. doi:10.1093/ije/31.3.638
[15] Goodman, K.J., Correa, P., Tengana, A.H. et al. (1996) Helicobacter pylori infection in the Colombian Andes: A population-based study of transmission pathways. American Journal of Epidemiology, 144, 290-299. doi:10.1093/oxfordjournals.aje.a008924
[16] Goodman, K.J., Correa, P., Tengana, A.H., DeLany, J.P. and Collazos, T. (1997) Nutritional factors and Helicobacter pylori infection in Colombian children. Journal Pediatric Gastroenterolgy Nutrition, 25, 507-515. doi:10.1097/00005176-199711000-00004
[17] Jarosz, M., Dzieniszewski, J., Dabrowska-Ufniarz, E., Wartanowicz, M., Ziemlanski, S. and Reed, P.I. (1998) Effects of high dose vitamin C treatment on Helicobacter pylori infection and total vitamin C concentration in gastric juice. European Journal of Cancer Prevention, 7, 449-454. doi:10.1097/00008469-199812000-00004
[18] Graham, D.Y., Malaty, H.M., Evans, D.G., Evans, D.J. Jr, Klein, P.D. and Adam, E. (1991) Epidemiology of Helicobacter pylori in an asymptomatic population in the United States. Effect of age, race, and socioeconomic status. Gastroenterology, 100, 1495-1501.
[19] Perry, S., de la Luz Sanchez, M., Yang, S., Haggerty, T.D., Hurst, P., Perez-Perez, G. and Parsonnet, J. (2006) Gastroenteritis and transmission of Helicobacter pylori infection in households. Emerging Infectious Diseases, 12, 1701-1708. doi:10.3201/eid1211.060086
[20] Mégraud, F. (1995) Transmission of Helicobacter pylori: Faecal-oral versus oral-oral route. Aliment Pharmacology Therapy, 9, 85-91.
[21] Lee, J.H., Shim, J.S., Lee, J.S., Kim, M.K., Chung, M.S., Kim, K.H. (2006) Pectin-like acidic polysaccharide from Panax ginseng with selective antiadhesive activity against pathogenic bacteria. Carbohydrate Research, 341, 11541163.
[22] Silva, O., Viegas, S., de Mello-Sampayo, C., Costa, M.J., Serrano, R., Cabrita, J. and Gomes, E.T. (2012) Anti-Helicobacter pylori activity of Terminalia macroptera root. Fitoterapia, 83, 872-876. doi:10.1016/j.fitote.2012.03.019
[23] Stalheim, T., Balance, S., Christensen, B.E. and Granum, P.E. (2009) Sphagnan—A pectin-like polymer isolated from Sphagnum moss can inhibit the growth of some typical food spoilage and food poisoning bacteria by lowering the pH. Journal of Applied Microbiology, 106, 967-976. doi:10.1111/j.1365-2672.2008.04057.x
[24] Painter, T.J. (1991) Lindow man, Tollund man and other peat-bog bodies—The preservative and antimicrobial action of sphagnan, a reactive glycuronoglycan with tanning and sequestering properties. Carbohydrate Polymers, 15, 123-142. doi:10.1016/0144-8617(91)90028-B

  
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