Studies on Extracting Microcystin-Lr from Microcystis Aeruginosa by Water Bath

Li Fang, Wenqing Liu, Nanjing Zhao, Jingbo Duan, Zhigang Wang, Yujun Zhang, Xue Xiao, Jing Liu, Gaofang Yin, Chaoyi Shi

Key Laboratory of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, 350 Shu Shan Hu Road, Hefei, Anhui 230031, China.

DOI: 10.4236/jep.2013.41B013                

Abstract

Different temperatures of water bath was used to extract the intracellular microcystin-LR(MC-LR) of Microcystis aeruginosa. Researching the extraction efficiency under the suitable temperature, so that it could find out the best temperature and time for extracting MC-LR from Microcystis aeruginosa cells. Five equal Microcystis aeruginosa was used to find out the best temperature, extracting at 60℃, 70℃, 80℃, 90℃ and 100℃ for 15 minutes, respectively. Results showed that the content of MC-LR extracted with the water under 100℃ was the highest. But meanwhile, the type and the content of impurities was the highest, too. In addition, another six equal Microcystis aeruginosa was extract with the water under 100℃ for 5min, 10 min, 15 min, 20 min, 25 min and 30 min respectively. It was proved that 20 minutes was enough for extracting MC-LR from Microcystis aeruginosa, no long time was needed.

Keywords

Boiling Water Bath, Extract, Microcystin-LR

Conflicts of Interest

The authors declare no conflicts of interest.

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