In Vitro Study of Carbamate-Linked Cationic Lipid for Gene Delivery Against Cervical Cancer Cells

Defu Zhi, Shuibao Zhang, Yinan Zhao, Shaohui Cui, Bing Wang, Huiying Chen, Defu Zhi, Defeng Zhao

SEAC-ME Key Laboratory of Biochemistry Engineering, Dalian Nationalities University, Dalian, China.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China.
tate Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian, China.

DOI: 10.4236/ampc.2012.24B058                

Abstract

Design and synthesis of a carbamate-linked cationic lipid DDCTMA (N-[1-(2,3-didodecylcarbamoyloxy)propyl]-N,N,N-trimethylammonium iodide)  as gene delivery carriers was described in this work. The transfection efficiency of cationic liposome increased dramatically with the increase in the content of DOPE. In addition, the transfection efficiency of some of cationic lipoplexes was superior or parallel to that of two commercial transfection agents, Lipofectamine2000 and DOTAP. The carbamate-linked cationic lipid DDCTMA/DOPE may be a promising gene carrier that has high transfection efficiency as well as low cytotoxicity.

Keywords

Cationic Lipids; DNA Condensation; Gene Delivery; Transfection Efficiency

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	L. Ciani, S. Ristori, A. Salvati, L. Calamai and G. Martini, “DOTAP/DOPE and DC-Chol/DOPE lipoplexes for gene delivery: zeta potential measurements and electron spin resonance spectra,” Biochim. Biophys. Acta. vol. 1664, pp. 70-79, July 2004.
[2]	P. L., Felgner and G. M. Ringold, “Cationic liposomemediated transfection,” Nature. vol. 337, pp. 387-388, January 1989.
[3]	M. A., Mintzer and E. E., Simanek, “Nonviral vectors for gene delivery,” Chem. Rev. vol. 109, pp. 259-302, April 2009.
[4]	D. F. Zhi, S. B. Zhang, B. Wang, Y. N. Zhao, B. L. Yang and S. J. Yu, “Transfection efficiency of cationic lipids with different hydrophobic domains in gene delivery,” Bioconjugate Chem. vol. 21, pp. 563-577, January 2010.
[5]	D. F. Zhi, F. Qureshi, S. B. Zhang, Y. N. Zhao, S. H. Cui, B. Wang, H. Y. Chen, Y. H. Wang and D. F. Zhao, “Synthesis and biological activity of carbamate-linked cationic lipids for gene delivery in vitro,” Bioorg. Med. Chem. Lett., vol. 22, pp. 3837-3841, February 2012.
[6]	T. Yoshioka, S. Yoshida, T. Kurosaki, M. Teshima, K. Nishida, J. Nakamura, M. Nakashima, H. To, T. Kitahara and H. Sasaki, “Cationic liposomes-mediated plasmid DNA delivery in murine hepatitis induced by carbon tetrachloride,” J. Liposome Res. vol. 19, pp. 141-147, June 2009.
[7]	S., Fletcher, A., Ahmad, E., Perouzel, M. R., Jorgensen and A. D. Miller, “A dialkanoyl analogue of DOPE improves gene transfer of lower-charged, cationic lipoplexes.” Org. Biomol. Chem., vol. 4, pp. 196-199, December 2006.