Induction of Oxidative Stress in Male Rats Subchronically Exposed to Electromagnetic Fields at Non-Thermal Intensities

Albert Achudume, Bill Onibere, Funso Aina, Paschal Tchokossa

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DOI: 10.4236/jemaa.2010.28064                

Abstract

To investigate the oxidative stress-inducing potential of non-thermal electromagnetic fields in rats. Male Wister rats were exposed to electrical field intensity of 2.3 ± 0.82 µV/m . Exposure was in three forms: continuous waves, or modulated at 900 MHz or modulated GSM-nonDTX. The radio frequency radiation (RFR) was 1800 MHz, specific absorption radiation (SAR) (0.95-3.9 W/kg) for 40 and/or 60 days continuously. Control animals were located > 300 m from base station, while sham control animals were located in a similar environmental conditions, but in the vicinity of a non-functional base station. The rats were assessed for thiobarbituric and reactive species (TBARS), reduced glutathione (GSH) content, catalase activity, glutathione reductase (GR) and glucose residue after 40 and 60 days of exposure. At 40 days, electromagnetic radiation failed to induce any significant alterations. However, at 60 days of exposure various attributes evaluated decreased. The respective decreases in both nicotinamide adenine dinucleotide phosphate (NADPH) and Ascorbate- linked lipid peroxidation (LPO) with concomitant diminution in enzymatic antioxidative defense systems resulted in decreased glucose residue. The present studies showed some biochemical changes that may be associated with a prolong exposure to electromagnetic fields and its relationship to the activity of antioxidant system in rat Regular assessment and early detection of antioxidative defense system among people working around the base stations are recommended.

Keywords

Electromagnetic field, telecommunication base station, Oxidative stress, antioxidant system

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	W. R. Adey, “Tissue Interactions with Non-Ionizing Electromagnetic Fields Physiological Review,” Vol. 61, No. 2, 1981, pp. 435-514.
[2]	N. Nikolaevich, A. Igorevna and G. Vasil, “Influence of High-Frequency Electromagnetic Radiation at Non-Ther- mal Intensities on the Human Body,” No Place to Hide, Vol. 3, No. 1, 2001, pp. 607-613
[3]	A. Balmori, “Possible Effects of Electromagnetic Fields from Phone Masts on a Population of White Stork,” Electromagnetic Biology and Medicine, Vol. 24, No. 2, 2005, pp. 109-119.
[4]	R. Santini, P. Santini, P. Ruz, JM. Danze and M. seigne, “Survey Study of People Living in the Vicinity of Cellular Phone Base,” Journal of Electromagnetic Biology and Medicine, Vol. 22, No. 1, 2002, pp. 41-49.
[5]	J. Sch?z, E. B?hler, G. Berg, B. Schlehofer, I. Hettinger, K. Schlaefer. J. wahrendorf, K. Kunna-Grass and M. Blettner, “Cellular phones, cordless phones and the risks of glioma and meningioma,” (Interphone study Group, Germany). American Journal of Epidemiology, Vol. 163, No. 6, 2006, pp. 512-520.
[6]	A. Auvinen, K. G. Blaasaas, E. Cardis, H. C. Christensen, M. Feychting, S. J. Hepworth, C. Johansen, L. Klaeboe, S. Lonn, P. A. McKinney, K. Muir, J. Ra-tanen, T. Salminen, J. Thomsen and T. Tynes, “Mobile Phone Use and Risk of Acoustic Neuroma; Results of the Interphone Case- Control Study in 5 North European Countries,” British Journal of Cancer 3, Vol. 93, No. 7, 2005, pp. 842-848.
[7]	A. A. Marino, E. Nilsen and C. Frilot, “Non-Linear Cha- Nges in Brain Electrical Activity Due to Cell Phone Radiation,” Bioelectromagnetics. Vol. 24, No. 5, 2003, pp. 339-346.
[8]	C. Daniells, I. Duce, D. Thomas, P. Sewell, J. Tattersall, and D. De Pomerai, “Transgenic Nematodes as Biomonitors of Microwave-Induced Stress,” Mutation Research, Vol. 399, No. 1, 1998, pp. 55-64.
[9]	S. K. Dutta, B. Ghosh and C. F. Blackman, “Radiofrequ- Ency Radiation-Induced Calcium Ion Efflux Enhancement from Human and Other Neuroblastoma Cells in Culture,” Bioelectromagnetics, Vol. 10, No. 2, 1989, pp. 197-202.
[10]	K. I. Obukhan, “The Effects of Ultrahigh-Frequency Radiation on Adaptation Thresholds and the Damages to Blood System Cells,” Lik Sprava, Vol. 2, No. 7, 1998, pp. 71-73
[11]	S. Sarkar, S. Ali and J. Behari, “Effect of Low Power Microwave on the Mouse Genome: A Direct DNA Analysis,” Mutation Research, Vol. 320, No. 1-2, 1994, pp. 141-147.
[12]	S. L?nn, A. Ahlbom, P. Hall and M. Feychting, “Long- Term Mobile Phone Use and Brain Tumor Risk,” American Journal of Epidemiology, Vol. 161 No. 6, 2005, pp. 526-535.
[13]	T. E. Belousova and R. A. Karginat-Terent’eva, “Adrenergic nerve plexuses of heart and adrenal and myocardial catecholamines of spontaneously hypertensive rats under the influence of electro-magnetic irradiation in the millimeter range,” Morfologia, Vol. 115, No. 1, 1999, pp. 16- 18.
[14]	M. Petrides, “Exposure to Electromagnetic Fields by Using Cellular Telephones and its Influence on the Brain,” Neuro Report, Vol. 11, No. 15, 2000, p. F15.
[15]	K. J. Fernie, D. M. Bird, R. D. Dawson and P. C. Lague, “Effects of Electromagnetic Fields on the Reproductive Success of American Kestrels,” Journal of Physiology, Biochemical and Zoology, Vol. 73, No. 1, 2000, pp. 60-65.
[16]	G. Abdel-Rassoul, O. Abou EL-Fateh, M. Abou Salam, A. Michael, F. Farahat, M. EL-Batanouny and E. Salem, “Neurobehavioral Effects among Inhabitants around Mobile Phone Base Stations,” Neuro Toxicology, Vol. 28, No. 2, 2007, pp. 434-440.
[17]	B. Tavazzi, D. Pierro, A. M. Amorini, et al, “Energy Metabolism and Lipid Peroxidation of Human Erythrocytes as a Function of Increased Oxidative Stress,” European Journal of Biochemistry, Vol. 267, No. 3, 2000, pp. 684- 689.
[18]	J. Everse and N. Hsia, “The Toxicities of Native and Modified Hemoglobins,” Free Radiological Biology and Medi- cine, Vol. 22, No. 6, 1997, pp. 1075-1099.
[19]	M. Havas, “Dirty Electricity Elevates Blood Sugar among Electrically Sensitive Diabetics and May Explain Brittle Diabetes,” Electromagnetic Biology and Medicine, Vol. 27, No. 2, 2008, pp. 135-146.
[20]	M. Younnes and C. P. Siegers, “Lipid Peroxidation as a Consequence of Glutathione Depletion in Rat and Mouse Liver,” Research of Common Chemical Pathology and Pharmacology, Vol. 27, No. 1, 1980, pp. 119-128.
[21]	H. K. Prins and J. A. Loos “Glutathione,” In: J. G. Yunis, Ed., Biochemical Methods in Red Cell Genetics, Academic Press, New York, 1969, pp. 127-129.
[22]	H. E. Aebi, “Catalase,” In: H. U. Bergmeyer, J. Bergmeyer, and M. GraBL, Ed., Methods of Enzymatic Analysis, Verlag Chemie, Weinheim, 1983, pp. 273-286.
[23]	D. M. Goldberg and R. J. Spooner, “Glutathione Reductase,” In: H. U. Bergmeyer, J. Bergmeyer and M. GraBL, Eds., Methods of Enzymatic Analysis, Verlag Chemie, Wenham, 1983, pp. 258-265.
[24]	N. A. Nelson, “Photometric Adaptation of Somogy Me- thod for the Determination of Glucose,” Journal of Biological Chemistry, Vol. 153, No. 1, 1944, pp. 375-380.
[25]	R. Santini, P. Santini, J. M. Danze, P. Le Ruz and M. Seigne, “Symptoms Experienced By People in Vicinity of Base Stations: II/Incidences of Age, Duration of Exposure, Location of Subjects in Relation to the Antennas and Other Electromagnet Factors,” Journal of Pathology and Biology, Vol. 51, No. 7, 2003, pp. 412-415.
[26]	R. Santini, P. Santini, J. M. Danze, P. Le Ruz and M. Seigne, “Study of the Health of People Living in the Vicinity of Mobile Phone Base Stations: 1. Influence of Distance and Sex,” Journal of Pathology and Biology, Vol. 50, No. 4, 2005, pp. 369-373.
[27]	J. Eritsland, “Safety Considerations of Polyunsaturated Fatty Acids,” American Journal of Clinical Nutrition, Vol. 71, No. 1, 2000, pp. 1975-1980.
[28]	G. Van Ginkel and A. Sevanian, “Lipid Peroxidation-In- duced Membrane Structural Alterations,” Methods Enzymol, Vol. 233, No. 2, 1994, pp. 273-288.
[29]	G. Hyland, “Physics and Biology of Mobile Telephony,” Lancet, Vol. 356, No. 12, 2005, pp. 1833-1836.
[30]	M. R??sli, M. Moser, Y. Baldinini, M. Meier and C. B. Fahrlander, “Symptoms of Ill Health Ascribed to Electromagnetic Field Exposure—A Questionnaire Survey International,” Journal of Hygiene and Environmental Health, Vol. 207, No. 2, 2004, pp. 141-159.
[31]	Z. Gregus and C. D. Klaassen, “Mechanism of Toxicity,” In: C. D. Klaassen, Ed., Casarett and Doull’s Toxicology: The Basic Science of Poisons, McGraw-Hill, New York, 2001, pp. 35-81.