Physiological and Biochemical Basis of Water-Deficit Stress Tolerance in Pearl Millet Hybrid and Parents

T. Vijayalakshmi, Y. Varalaxmi, S. Jainender, S. K. Yadav, M. Vanaja, N. Jyothilakshmi, M. Maheswari

Division of Crop Sciences, Central Research Institute for Dryland Agriculture, Hyderabad, India.

DOI: 10.4236/ajps.2012.312211                

Abstract

The present investigation was aimed to understand the physiological and biochemical basis of water-deficit stress tolerance in pearl millet [Pennisetum glaucum (L.) R. Br.] hybrid ICMH 356 and its parents ICMR 356 (♂) and ICMB 88004 (♀) in response to and recovery from drought stress and also to comprehend crop adaptation under dryland conditions. A field experiment was conducted in a split plot design with moisture levels as the main plot and genotypes as the sub plots. A comparative analysis of hybrid and parents under well-watered and water-deficit stress conditions revealed that the hybrid was superior over the parents in terms of leaf water relations, excised leaf water retention capacity, accumulation of compatible solutes, photosynthesis, membrane stability index and antioxidative enzyme viz., superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), ascorbate peroxidase (APX) and guaiacol peroxidase (GPOX) activities. ANOVA for these parameters was also found to be significant for genotypes, treatments and their interactions at 0.01% level. Maintenance of superiority in terms of these physiological and biochemical parameters coupled with better recovery ability upon stress relief are crucial physiological mechanisms contributing to water deficit stress tolerance in pearl millet. Simple correlation coefficient analysis revealed significant positive association of yield at 0.01% level with relative water content, leaf water potential, stomatal conductance, photosynthesis, proline, total soluble sugars, free amino acids, membrane stability index, leaf area index and total biomass, while a significant negative association with solute potential and malondialdehyde content, under water-deficit stress clearly indicated that such relationships can be positively attributed to drought tolerance.

Keywords

Pearl Millet; Water Relations; Osmotic Adjustment; Membrane Stability Index; AntioxidativeEnzymes; Seed Yield

Conflicts of Interest

The authors declare no conflicts of interest.

References

[1]	M. M. Chaves, J. Flexas and C. Pinheiro, “Photosynthesis under Drought and Salt Stress: Regulation Mechanisms from Whole Plant to Cell,” Annals of Botany, Vol. 103, No. 4, 2009, pp. 551-560. doi:10.1093/jxb/erp069
[2]	J. M. Morgan and R. W. King, “Association between Loss of Leaf Turgor, Abscisic Acid Levels and Seed Set in Two Wheat Cultivars,” Australian Journal of Plant Physiology, Vol. 11, No. 3, 1984, pp. 143-150. doi:10.1071/PP9840143
[3]	L. Szabados and A. Savoure, “Proline: A Multifunctional Amino acid,” Trends in Plant Sciences, Vol. 15, No. 2, 2009, pp. 89-97.
[4]	M. Ashraf, “Inducing Drought Tolerance in Plants,” Biotechnology Advances, Vol. 28, No. 1, 2009, pp. 169-183.
[5]	M. Helena and C. D. Carvalho, “Drought Stress and Reactive Oxygen Species,” Plant Signalling and Behaviour, Vol. 3, No. 3, 2008, pp. 156-165.
[6]	C. Bowler, V. M. Montagu and D. Inzé, “Superoxide Dismutases and Stress Tolerance,” Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 43, No. 1, 1992, pp. 83-116. doi:10.1146/annurev.arplant.43.1.83
[7]	G. Govind, H. V. T. Gowda, P. J. Kalaiarasi, D. R. Iyer, S. K. Muthappa, S. Nese and U. K. Makarla, “Identification and Functional Validation of a Unique Set of Drought Induced Genes Preferentially Expressed in Response to Gradual Water Stress in Peanut,” Molecular Genetics and Genomics, Vol. 281, No. 6, 2009, pp. 591-605. doi:10.1007/s00438-009-0441-y
[8]	I. S. Khairwal, S. K. Yadav, K. N. Rai, H. D. Upadhyaya, D. Kachhawa, B. Nirwan, R. Battacharjee, B. S. Rajpurohit, C. J. Dangaria and Srikant, “Evaluation and Identification of Promising Pearl Millet Germplasm for Grain and Fodder Traits,” Journal of SAT Agricultural Research, Vol. 5, No. 1, 2007, pp. 1-6.
[9]	B. R. Whan, W. K. Anderson and R. F. Gilmour, “A Role for Physiology in Breeding for Improved Wheat Yield under Drought Stress,” In: E. Acevedo, A. P. Conesa, Pmonneveux and J. P. Srivastava, Ed., Physiology-Breeding of Winter Cereals for Stressed Mediterranean Environments, I. N. R. A, les Collogues, Montpellier, 1991, pp. 179-191.
[10]	H. D. Barrs and P. E. Weatherly, “A Re-examination of the Relative Turgidity Technique for Estimating Water Deficits in Leaves,” Australian Journal of Biological Science, Vol. 15, No. 3, 1962, pp. 413-428.
[11]	P. F. Scholander, H. T. Hammel, E. D. Bradstreet and E. A. Himminosen, “Sap Pressure on Vascular Plants,” Science, Vol. 148, No. 3668, 1966, pp. 339-346. doi:10.1104/pp.41.3.529
[12]	R. C. Babu, M. S. Pathan, A. Blum and T. N. Nguyen, “Comparison of Measurement Methods of Osmotic Adjustment in Different Rice Cultivars,” Crop Science, Vol. 39, No. 1, 1999, pp. 150-158. doi:10.2135/cropsci1999.0011183X003900010024x
[13]	N. C. Turner, J. C. Toole, R. T. Cruz, E. B. Yambao, S. Ahmad, O. S. Namuco and M. Dingkuhn, “Response of Seven Diverse Rice Cultivars to Water Deficit. II Osmotic Adjustment, Leaf Elasticity, Leaf Extension, Leaf Death, Stomatal Conductance and Photosynthesis,” Field Crops Research, Vol. 13, 1986, pp. 273-286. doi:10.1016/0378-4290(86)90028-6
[14]	G. D. Rao, M. Vanaja, K. C. Lakkneni and P. R. Reddy, “Stability of Excised Leaf Water Retention Capacity (ELWRC) Technique for Screening Castor Genotypes,” Journal of Oilseed Research, Vol. 15, No. 2, 1998, pp. 280-287.
[15]	L. S. Bates, R. P. Waldren and I. D. Teare, “Rapid Determination of Free Proline for Water Stress Studies,” Plant Soil, Vol. 39, No. 1, 1973, pp. 205-207. doi:10.1007/BF00018060
[16]	M. Dubois, K. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith, “A Colorimetric Method for the Determination of Sugars,” Nature, Vol. 168, No. 4265, 1951, pp. 167-168. doi:10.1038/168167a0
[17]	H. Rosen, “A Modified Ninhydrin Colorimetric Analysis for Amino Acids,” Archives of Biochemistry and Biophysics, Vol. 67, No. 1, 1957, pp. 10-15. doi:10.1016/0003-9861(57)90241-2
[18]	J. C. O’Toole and J. G. Real, “Estimation of Aerodynamic and Crop Resistances from Canopy Temperature,” Agronomy Journal, Vol. 78, No. 2, 1986, pp. 305-310. doi:10.2134/agronj1986.00021962007800020019x
[19]	R. L. Heath and L. Packer, “Photo Peroxidation in Isolated Chloroplasts. I Kinetics and Stoichiometry of Fatty Acid Peroxidation,” Archives of Biochemistry and Biophysics, Vol. 125, No. 1, 1968, pp. 189-198. doi:10.1016/0003-9861(68)90654-1
[20]	G. S. Premachandra, H. Saneoka, M. Kanaya and S. Ogata, “Cell Membrane Stability and Leaf Surface Wax Content as Affected by Increasing Water Deficits in Maize,” Journal of Experimental Botany, Vol. 42, No. 2, 1991, pp. 167-171. doi:10.1093/jxb/42.2.167
[21]	R. K. Sairam, “Effect of Moisture Stress on Physiological Activities of Two Contrasting Wheat Genotypes,” Indian Journal of Experimental Biology, Vol. 32, 1994, pp. 584- 593.
[22]	M. M. Bradford, “A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Utilizing the Principle of Protein-Dye Binding,” Annals of Biochemistry, Vol. 72, No. 1-2, 1976, pp. 248-254. doi:10.1016/0003-2697(76)90527-3
[23]	R. S. Dhindsa, P. P. Dhindsa and T. A. Throne, “Leaf Senescence; Correlated with Increased Levels of Membrane Permeability and Lipid Peroxidation and Decreased Levels of Superoxide Dismutase and Catalase,” Journal of Experimental Botany, Vol. 32, 1981, pp. 93-101. doi:10.1093/jxb/32.1.93
[24]	A. Claiborne, “Catalase Activity,” In: R. A. Greenwald, Ed., CRC Handbook of Methods for Oxygen Radical Research, Boca Raton, 1985, pp. 283-284.
[25]	I. K. Smith, T. L. Vierheller and C. A. Thorne, “Assay of Glutathione Reductase in Crude Tissue Homogenates Using 5.5-Dithiobis(2-nitobenzoic acid),” Annals of Biochemistry, Vol. 175, No. 2, 1988, pp. 408-413. doi:10.1016/0003-2697(88)90564-7
[26]	Y. Nakano and K. Asada “Hydrogen Peroxide is Scavenged by Ascorbate Specific Peroxides in Spinach Chlooplasts,” Plant Cell Physiology, Vol. 22, No. 5, 1981, pp. 867-880.
[27]	B. Chance and A. C. Maehly “Assay of Catalase and Peroxidases,” Methods in Enzymology, Vol. 2, No. 11, 1955, pp. 764-775. doi:10.1016/S0076-6879(55)02300-8
[28]	G. W. Snedecor and W. G. Cochran, “Statistical Methods,” Oxford and IBH Publishing Co., Calcutta, 1967.
[29]	I. E. Henson,V. Mahalakshmi, F. R. Bidinger and G. Alagarswamy, “Osmotic Adjustment to Water Stress in Pearl Millet (Pennisetum glaucum [L.] Leeke) under Field Conditions,” Plant, Cell and Environment, Vol. 5, No. 2, 1982, pp. 147-154. doi:10.1111/1365-3040.ep11571559
[30]	N. Thurling and L. D. Das “The Relationship for Pre-Anthesis Development and Seed Yield of Spring Rape (Brassica napus L.),” Australian Journal of Agricultural Research, Vol. 31, No. 1, 1980, pp. 25-36. doi:10.1071/AR9800025
[31]	S. K. Yadav, N. J. Lakshmi, M. Maheswari, M. Vanaja and B. Venkateswarlu, “Influence of Water Deficit at Vegetative, Anthesis and Grain Filling Stages on Water Relation and Grain Yield in Sorghum,” Indian Journal of Plant Physiology, Vol. 10, No. 1, 2005, pp. 20-24.
[32]	I. E. Henson, “Osmotic Adjustment to Water Stress in Pearl Millet (Pennisetum glaucum(L.) Leeke) in a Controlled Environment,” Journal of Experimental Botany, Vol. 33, No. 1, 1982, pp. 78-87. doi:10.1093/jxb/33.1.78
[33]	A. Gonzalez, I. Martin and L. Ayerbe, “Yield and Osmotic Adjustment Capacity of Barley under Terminal Water-Stress Conditions,” Journal of Agronomy and Crop Science, Vol. 194, No. 2, 2008, pp. 81-91.
[34]	I. Slama, S. Tayachi and A. Jadey, “Differential Response to Water Deficit Stress in Alfalfa (Medicago sativa) Cultivars: Growth, Water Relations, Osmolyte Accumulation and Lipid Peroxidation,” Journal of Biotechnology, Vol. 10, No. 72, pp. 16250-16259.
[35]	A. Takele, “Differential Responses of Electrolyte Leakage and Pigment Compositions in Maize and Sorghum after Exposure to and Recovery From Pre- and Post- Flowering Dehydration,” Agricultural Science of China, Vol. 9, No. 6, 2010, pp. 813-824. doi:10.1016/S1671-2927(09)60159-0
[36]	H. E. Patil, M. K. Mahatma, N. J. Patel, R. Bhatnagar and G. C. Jadeja, “Differential Response of Pearl Millet Hybrids to Water Stress in Relation to Antioxidant Enzymes and Proline,” Indian Journal of Plant Physiology, Vol. 10, No. 4, 2005, pp. 344-348.
[37]	W. Tezara, W. J. Mitchell, S. D. Driscoll and D. W. Lawlor, “Effects of Water Deficit and Its Interaction with CO2 Supply on the Biochemistry and Physiology of Photosynthesis in Sunflower,” Journal of Experimental Botany, Vol. 53, No. 375, 2002, pp. 1781-1791. doi:10.1093/jxb/erf021
[38]	R. Khanna-Chopra and M. Maheswari, “Effect of Altering Source Availability on Expression of Sink Capacity in a Maize Hybrid and its Parents,” European Journal of Agronomy, Vol. 9, No. 1998, pp. 101-107. doi:10.1016/S1161-0301(98)00028-8
[39]	A. Hussain, M. R. Chaudhry, A. Wajid, A. Ahmad, M. Rafiq, M. Ibrahim and A. R. Goheer, “Growth, Yield and Radiation Use Efficiency of Various Wheat Cultivars,” International Journal of Agricultural Science, Vol. 6, No. 6, 2004, pp. 1074-1079.