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Schiff base SH11 with tuberculostatic and radical scavenging activities against INH-induced oxidative hepatic damage

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DOI: 10.4236/abb.2012.327130    3,422 Downloads   7,012 Views   Citations

ABSTRACT

By in vitro visible electron paramagnetic resonance (EPR) spectrophotometry method we demonstrated that N-isonicotinoyl-N’-(3-etoxy-2-hydroxybenzaldehyd) hydrazone (SH11) exhibits radical scavenging activity (SSA). Malondialdehyde (MDA) in mice treated with INH was increased (2.578 ± 0.349 mM vs. 2.024 ± 0.164 μM, p < 0.001), while both superoxide dismutase (SOD) (1.583 ± 0.562 USOD/mg Pr vs. 2.273 ± 0.317 USOD/mg Pr, p < 0.05) and catalase (CAT) (30.176 ± 7.300 UCAT/mg Pr, vs. 47.070 ± 16.490 UCAT/mg Pr, p < 0.05) were decreased, compared to the untreated controls. The combination INH + SH11 (30 mg/kg p.o.) showed reduced levels of MDA, compared to the INH-treated (mean 2.291 ± 0.025 μM than 2.578 ± 0.349, p < 0.05). The combination with the lowest reduction of SOD compared to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11, but there was a significant difference in SOD activities between the group treated with this combination and the untreated controls (p < 0.05). The most effective combination, with CAT levels, close to the controls was 151 mg/kg i.p. INH + 30 mg/kg p.o. SH11.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Georgieva, N. , Yaneva, Z. , Nikolova, G. and Simova, S. (2012) Schiff base SH11 with tuberculostatic and radical scavenging activities against INH-induced oxidative hepatic damage. Advances in Bioscience and Biotechnology, 3, 1068-1075. doi: 10.4236/abb.2012.327130.

References

[1] Mahesh, C., Shailesh, P. and Mitesh, J. (2010) Recent Development and Future Perspective of Antitubercular Therapy. Anti-Infective Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Infective Agents), 9, 59-103. doi:10.2174/187152110791383779
[2] Van Halsema, C.L., Fielding, K.L., Chihota, V.N., Russell, E.C., Lewis, J.J., Churchyard, G.J. and Grant, A.D. (2010) Tuberculosis outcomes and drug susceptibility in individuals exposed to isoniazid preventive therapy in a high HIV prevalence setting. AIDS, 24, 1051-1055. doi:10.1097/QAD.0b013e32833849df
[3] Ravi, V., Patel, S.S., Verma, N.K., Dutta, D. and Saleem, T.S.M. (2010) Hepatoprotective activity of Bombax ceiba linn against isoniazid and rifampicin-induced toxicity in experimental rats. International Journal of Applied Research in Natural Products, 3, 19-26.
[4] Hearn, M.J., Cynamon, M.H., Chen, M.F., Coppins, R., Davis, J., Kang, H.J., Noble, A., Tu-Sekine, B., Terrot, M.S., Trombino, D., Thai, M., Webster, E.R. and Wilson, R. (2009) Preparation and antitubercular activities in vitro and in vivo of novel Schiff bases of isoniazid. European Journal of Medicinal Chemistry, 44, 4169-4178. doi:10.1016/j.ejmech.2009.05.009
[5] Georgieva, N. and Gadjeva, V. (2002) Isonicotinoyl-hydrazone analogs of isoniazid: Relationship between superoxide scavenging and tuberculostatic activities. Biochemistry (Moscow), 67, 588-591. doi:10.1023/A:1015558514432 www.ncbi.nlm.nih.gov/pubmed/12059780
[6] Georgieva, N., Gadjeva, V., Tolekova, A. and Dimitrov, D. (2005) Hepatoprotective effect of isonicotinoylhydrazone SH7 against chronic isoniazid toxicity. Die Pharmazie, 60, 138-141. www.ncbi.nlm.nih.gov/pubmed/15739904
[7] Varbanova, S. and Georgieva, N. (1993) New isonicotino-ylhydrazones with tuberculostatic action. Veterinary Science, 27, 81-85.
[8] Geran, R.S., Greenberg, N.H., Macdonald, M.M., Schumacher, A.M. and Abbott, B.J. (1972) Protocols for screening chemical agents and natural products against animal tumors and other biological systems. Cancer Chemotherapy Reports, 13, 1-87.
[9] Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein measurement with the Folin Phenol Reagent. The Journal of Biological Chemistry, 193, 265-275. www.ncbi.nlm.nih.gov/pubmed/14907713
[10] Placer, Z.A., Cushman, L.L. and Jonson, B.C. (1966) Estimation of product of lipid peroxidation (Malonyl Dialdehide) in Biochemcal Systems. Analitical Biochemistry, 16, 359-364. doi:10.1016/0003-2697(66)90167-9
[11] Sun, Y., Oberley, L.W. and Li, Y. (1988) A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34, 497-500.
[12] Beers, R. and Sizer, T. (1952) Spectrophotometric metod for measuring the breakdown of hydrogenperoxide by catalase. The Journal of Biological Chemistry, 195, 133-138.
[13] Fridovich, I. (1975) Superoxide dismutases. Annual Review of Biochemistry, 44, 147-159. doi:10.1146/annurev.bi.44.070175.001051
[14] Bhadauria, S., Mishra, R., Kanchan, R., Tripathi, C., Srivastava, A., Tiwari, A. and Sharma, S. (2010) Isoniazid-induced apoptosis in HepG2 cells: Generation of oxidative stress and Bcl-2 down-regulation. Toxicology Mechanisms and Methods, 20, 242-251. doi:10.3109/15376511003793325
[15] Afanas’ev, I. (2010) Signaling of reactive oxygen and nitrogen species in diabetes mellitus. Oxidative Medicine and Cellular Longevity, 3, 361-373. doi:10.4161/oxim.3.6.14415
[16] Santhosh, S., Sini, T.K., Anandan, R. and Mathee, P.T. (2007) Hepatoprotective activity of chitosan against isoniazid and rifampicin-induced toxicity in experimental rats. European Journal of Pharmacology, 572, 69-73. doi:10.1016/j.ejphar.2007.05.059
[17] Georgieva, N.V. (2005) Oxidative stress as a factor of disrupted ecological oxidative balance in biological systems—A Review. Bulgarian Journal of Veterinary Medicine, 8, 1-11. http://tru.uni-sz.bg/bjvm/vol08-no01-01.pdf

  
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