26S proteasome inhibitors inhibit dexamethasone-dependent increase of tyrosine aminotransferase and tryptophan 2,3-dioxygenase mRNA levels in primary cultured rat hepatocytes

Mizuho Harashima, Masashi Hyuga, Youko Nagaoka, Chieko Saito, Minako Furukawa, Taiichiro Seki, Toyohiko Ariga, Nana Kawasaki, Shingo Niimi

Department of Nutrition and Physiology, Nihon University College of Bioresource Sciences, Fujisawa, Japan.
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences, Tokyo, Japan.

DOI: 10.4236/jbpc.2012.34043                

Abstract

Dexamethasone (Dex), a ligand for transcriptional enhancement of tyrosine aminotransferase (TAT) and tryptophan 2,3-dioxygenase (TO) genes, (100 nM) maximally increased these mRNA levels at 12 h and 7 h in primary cultured rat hepatocytes and the nuclear fraction, respectively. Lactacystin (5 μM) and epoxomicin (0.5 μM), 26S proteasome inhibitors, significantly suppressed the Dex-dependent maximum increase of TAT and TO mRNA levels in the cells and the nuclear fraction. Electrophoretic mobility shift assay demonstrated that lactacystin did not affect binding of glucocorticoid receptor to glucocorticoid responsive element. Furthermore, lactacystin did not affect the activation of GRE luciferase reporter by Dex transfected to the cells. The results demonstrate that 26S proteasome is positively involved in the Dex-dependent increase of TAT and TO mRNA levels in the cells and suggest that the mechanism of action of 26S proteasome may be degradation of some RNase(s), which breaks down TAT and TO mRNAs.

Keywords

Glucocorticoid; 26S proteasome Inhibitor; Tyrosine Aminotransferase; Tryptophan 2, 3-Dioxygenase Genes

Conflicts of Interest

The authors declare no conflicts of interest.

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