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Mitochondrial comparative proteomic analysis of sterile line and its maintain line of purple cytoplasmic rice (Oryza sativa)

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DOI: 10.4236/abb.2010.13020    4,493 Downloads   8,756 Views   Citations

ABSTRACT

CMS/Rf systems in rice (Oryza sativa) have long been exploited for hybrid breeding to enhance productivity. Ying xiang CMS/Rf system is a new type. In this study, a mitochondrial comparative proteomic analysis of Ying xiang Sterile Line and its Maintain Line was started for a comprehensive investigation of the mitochondrial proteins’ functions in rice cytoplasmic male sterility. Mitochondria were prepared from rice shoots grown in the dark. Proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF/ MS. Using Mascot, it was found that 7 proteins were not described previously for plant mitochondria, indicating novel mitochondrial functions. 3 of them were characterized.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Wei, L. , Fei, Z. , Wu, X. , Dong, H. , Zhou, P. and Zhang, J. (2010) Mitochondrial comparative proteomic analysis of sterile line and its maintain line of purple cytoplasmic rice (Oryza sativa). Advances in Bioscience and Biotechnology, 1, 145-151. doi: 10.4236/abb.2010.13020.

References

[1] Pring, D.R., Van, T.H. and Schertz, K.F. (1995) Cytoplasmic male sterility and organelle DNAs of sorghum. In: Levings, C.S., III and Vasil, I.K., Eds, Advances in Cellular and Molecular Biology of Plants, Kluwer Academic, Dordrecht, 461-495.
[2] Shinjyo, C. (1969) Cytoplasmic genetic male sterility in cultivated rice, Oryza sativa L. II. The inheritance of male sterility. Japanese Journal of Genetics, 44(3), 149-156.
[3] Lin, S. and Yuan, L. (1980) Hybrid rice breeding in China. Innovative Approaches to Rice Breeding, International Rice Research Institute, Los Banos, Philippines, 35-51.
[4] Rao, Y. (1988) Cytohistology of cytoplasmic male sterile lines in hybrid rice. In: Smith, W.H., Bostian, L.R. and Cervantes, E.P., Eds., Hybrid Rice, International Rice Research Institute, Manila, Philippines, 115-128.
[5] Li, J. and Yuan, L. (2000) Hybrid rice, genetics, breeding, and seed production. In: Janick, J., Ed, Plant Breeding Reviews, John Wiley & Sons, New York, 17, 15-158.
[6] Virmani, S.S. (2003) Advances in hybrid rice research and development in the tropics. In: Hanoi, Vietnam, S., Virmani, S., Mao, C. and Hardy, B., Eds, Hybrid Rice for Food Security, Poverty Alleviation, and Environmental Protection, International Rice Research Institute, Los Baňos, Philippines, 7-20.
[7] Dewey, R.E., Levings, C.S., III and Timothy, D.H. (1986) Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas malesterile cytoplasm. Cell, 44(3), 439-449.
[8] Levings, C.S., III (1990) The Texas cytoplasm of maize: Cytoplasmic male sterility and disease susceptibility. Science, 250(4983), 942-947.
[9] Hanson, M.R. (1991) Plant mitochondrial mutations and male sterility. Annual Review of Genetics, 25, 461-486.
[10] Hernould, M., Suharsono, S., Zabaleta, E., Carde, J.P., Litvak, S., Araya, A. and Mouras, A. (1998) Impairment of tapetum and mitochondria in engineered male-sterile tobacco plants. Plant Molecular Biology, 36(4), 499-508.
[11] Hanson, M.R. and Bentolila, S. (2004) Interaction of mitochondria and nuclear genes that affect male gametophyte development. The Plant Cell, 16(Suppl 1), S154-S169.
[12] Wang, Z., Zou, Y., Li, X., Zhang, Q., Chen, L., Wu, H., Su, D., Chen, Y., Guo, J., Luo, D., Long, Y., Zhong, Y. and Liu, Y. ( 2006) Cytoplasmic male sterility of rice with boro II cytoplasm is caused by a cytotoxic peptide and is restored by two related PPR motif genes via distinct modes of mRNA silencing. The Plant Cell, 18(3), 676-687.
[13] Rakwal, R., Agrawal, G.K. (2003) Rice proteomics: Current status and future perspectives. Electrophoresis, 24(19-20), 3378-3389.
[14] Tanaka, N., Mitsui, S., Nobori, H., Yanagi, K. and Komatsu, S. (2005) Expression and function of proteins during development of the basal region in rice seedlings. Molecular & Cellular Proteomics, 4(6), 796-808.
[15] Kim, D.S., Cho, D.S., Park, W.M., Na ,H.J. and Nam, H.G. (2006) Proteomic pattern-based analyses of light responses in Arabidopsis thaliana wild-type and photoreceptor mutants. Proteomics, 6(10), 3040-3049.
[16] Plomion, C., Lalanne, C., Claverol, S., Meddour, H., Kohler, A., Bogeat-Triboulot, M.B., Barre, A., Provost, G., Dumazet, H., Jacob, D., Bastien, C., Dreyer, E., Daruvar, A., Guehl, J.M., Schmitter, J.M., Martin, F. and Bonneu, M. (2006) Mapping the proteome of poplar and application to the discovery of drought-stress responsive proteins. Proteomics, 6(24), 6509-6527.
[17] Wei, L., Ding, Y., Liu, Y. and Yu, J. (2002) Microcalorimetric analysis of anthers of male sterile rice sterile line. Journal of Wuhan Botanical Research, 20(4),308-310.
[18] Hu, Y., Wu, Q., Liu, S., Wei, L., Chen, X., Yan, Z., Yu, J., Zeng, L. and Ding, Y. (2005) Study of rice pollen grains by multispectral imaging microscopy. Microscope Research and Technique, 68(6), 335-346.
[19] Wei, L., Ding, Y., Hu, Y. and Yu, J. (2002) Analysis of leaf proteins of zidao male sterile line by 2D-PAGE. Acta Genetica Sinica, 29(8), 696-699.
[20] Hu, Y., Wei, L., Liu, S., Yu, J. and Ding, Y. (2005) Analysis of male sterility-related protein of young panicle in rice (Oryza sativa L.) by two-Dimensional electrophoresis. Wuhan University Journal of Natural Sciences, 10(3), 597-601.
[21] Mignouna, H., Vermin, S.S. and Briquet, M. (1987) Mitochondrial DNA modification associated with cytoplasmic male sterility in rice. Theoretical and Applied Genetics, 74(5), 666-669.
[22] Zhang, Y., Fan, X., Chen, R., Xiao, Z., Feng, X., Tian, X. and Chen, Z. (2005) Comparative proteome analysis of untreated and Helicobacter pylori-treated HepG2. World Journal of Gastroenterology, 11(22), 3485-3489.
[23] Marilyn, E. and Robert, B. (1990) Respiration and mito- chondrial biogenesis in germinating embryos of maize. Plant Physiology, 93(1), 295-304.
[24] Hanson, M.R., Pruitt, K.D. and Nivison, H.T. (1989) Male sterility loci in plant mitochondrial genes. Oxford Surveys in Plant Molecular and Cellular Biology, 6, 61-85.
[25] Gagliardi, D. and Leaver, C.J. (1999) Polyadenylation accelerates the degradation of the mitochondria mRAN associated with cytoplasmic male sterility in sunflower. The EMBO Journal, 18(13), 3757-3766.
[26] Iwabuchi, M., Kyozuka, J. and Shimamoto, K. (1993) Processing followed by complete editing of an altered mitochondrial apt6 RNA restores fertility of cytoplasmic male sterile rice. The EMBO Journal, 12(4),1437-1446.
[27] Akagi, H., Sakamoto, M., Shinjyo, C., Shimada, H. and Fujimura, T. (1994) A unique sequence located down-stream from the rice mitochondrial apt6 may cause male sterility. Current Genetics, 25(1), 52-58.
[28] Yi, P., Wang, L., Sun, Q. and Zhu, Y. (2002) Discovery of mitochondria chimeric gene associated with male sterility of Honglian-rice. Chinese Science Bulletin, 47(9), 744-747.
[29] Peng, X., Li, F., Li, S. and Zhu, Y. (2009) Expression of a mitochondrial gene orfH79 from the CMS-HongLian rice inhibits Saccharomyces cerevisiae growth and causes excessive ROS accumulation and decrease in ATP. Biotechnology Letters, 31(3), 409-414.
[30] Pedersen, P.L. and Amzel, L.M. (1993) ATP synthases. Structure, reaction center, mechanism, and regulation of one of nature’s most unique machines. Journal of Biological Chemistry, 268(14), 9937-9940.
[31] Tedege, M. and Kuhlemeier, C. (1997) Aerobic fermentation during tobacco pollen development. Plant Molecu lar Biology, 35(3), 343-354.
[32] Runswick, M. and Walker, J. (1983) The amino acid sequence of the β-subunit of the ATP synthase from bovine heart mitochondria. Journal of Biological Chemistry, 258(5), 3081-3089.
[33] Zhang, H., Li, S., Yi, P., Wan, C., Chen, Z. and Zhu, Y. (2007) A Honglian CMS line of rice displays aberrant F0 of F0F1-ATPase. Plant Cell Reports, 26(7), 1065-1071.
[34] Hu, C., Sun, Q., Peng, X., Huang, Q., Wang, M., Li, S. and Zhu, Y. (2010) Flow cytometric analysis of mitochondrial populations in HL-CMS systems of rice under H2O2 stress. Protoplasma, 241(1-4), 91-98.
[35] Sun, Q., Hu, C., Hu, J., Li, S. and Zhu, Y. (2009) Quantitative proteomic analysis of CMS-related changes in Honglian CMS rice anther. The Protein Journal, 28(7-8), 341-348.
[36] Wei, L., Yan, Z. and Ding, Y. (2008) Mitochondrial RNA editing of F0-ATPase subunit 9 gene (atp9) transcripts of Yunnan purple rice cytoplasmic male sterile line and its maintainer line. Acta Physiologiae Plantarum, 30(5), 657-662.

  
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