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Mitochondrial comparative proteomic analysis of sterile line and its maintain line of purple cytoplasmic rice (Oryza sativa)

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DOI: 10.4236/abb.2010.13020    4,493 Downloads   8,756 Views   Citations


CMS/Rf systems in rice (Oryza sativa) have long been exploited for hybrid breeding to enhance productivity. Ying xiang CMS/Rf system is a new type. In this study, a mitochondrial comparative proteomic analysis of Ying xiang Sterile Line and its Maintain Line was started for a comprehensive investigation of the mitochondrial proteins’ functions in rice cytoplasmic male sterility. Mitochondria were prepared from rice shoots grown in the dark. Proteins were analyzed by two-dimensional electrophoresis and MALDI-TOF/ MS. Using Mascot, it was found that 7 proteins were not described previously for plant mitochondria, indicating novel mitochondrial functions. 3 of them were characterized.

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The authors declare no conflicts of interest.

Cite this paper

Wei, L. , Fei, Z. , Wu, X. , Dong, H. , Zhou, P. and Zhang, J. (2010) Mitochondrial comparative proteomic analysis of sterile line and its maintain line of purple cytoplasmic rice (Oryza sativa). Advances in Bioscience and Biotechnology, 1, 145-151. doi: 10.4236/abb.2010.13020.


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