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Solid state fermentation of pomegranate seed for lovastatin production: a bioprocessing approach

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DOI: 10.4236/abb.2012.35083    4,891 Downloads   8,338 Views   Citations

ABSTRACT

This study reports lovastatin production by solid state fermentation using pomegranate seeds as a substrate. Six different fungal strains and several agro-industrial wastes were selected and screened. Various physico-chemical parameters were optimized to improve lovastatin. Moreover, chemical mutation was systematically employed to enhance lovastatin yield on selected strains. Productivity of 3 ± 0.06 mg lovastatin/gm dfm was obtained prior to optimization. One factor a time followed by Response Surface Methodology (RSM) gave 4.2 ± 0.10 mg lovastatin/gm dfm yield in an optimized setup with pomegranate seed powder (5 gms), KH2PO4 (0.1% w/v), glucose (5% w/v), moisture (60% w/w), pH 5 in a 15 days fermentation cycle. The production was further increased to 6.5 ± 0.08 mg lovastatin/gm dfm through chemical mutation of the strain. This process is simple and reproducible for the production of lovastatin using pomegranate seed as an agro-industrial waste.

Conflicts of Interest

The authors declare no conflicts of interest.

Cite this paper

Naik, A. and Lele, S. (2012) Solid state fermentation of pomegranate seed for lovastatin production: a bioprocessing approach. Advances in Bioscience and Biotechnology, 3, 643-647. doi: 10.4236/abb.2012.35083.

References

[1] Jurenka, J. (2008) Therapeutic Applications of Pomegra nate (Punica granatum L.): A Review. Alternative medicine review, 13(2), 128-144.
[2] Aghsaghali, A.M. (2011) Evaluating potential nutritive value of pomegranate processing by-products for ruminants using in vitro gas production technique. ARPN Journal of Agricultural and Biological Science, 6(6), 45-51.
[3] EL-Nemr, S.E., Ismail, I.A., Raga, M. (1990) Chemical composition of juice and seeds of pomegranate fruit. Die Nahrung, 34(7), 601-606. doi:10.1002/food.19900340706
[4] Xu, B., Wang, Q., Jia, X., Sung, C. (2005) Enhanced lovastatin production by solid substrate fermentation of Monascus rubber. Biotechnology and bioprocess engineering, 10, 78-84.
[5] Manzoni, M., Bergomi, S., Rollini, M., Cavazzoni, V. (1999) Production of statins by filamentous fungi. Biotechnology letters, 21, 253-257. doi:10.1023/A:1005495714248
[6] Samiee, S.M., Moazami, N., Haghighi, S., Mohseni, F.A., Mirdamadi, S., Bakhtiari, M.R. (2003) Screening of lovastatin production by filamentous fungi. Iranian biomedical journal, 7(1), 29-33.
[7] Szakács, G., Morovján, G., Tengerdy, R.P. (1998) Production of lovastatin by a wild strain of Aspergillus terreus. Biotechnology Letters, 20(4), 411-415.
[8] Jia, Z., Zhang, X., Zhao, Y., Cao, X. (2010) Enhancement of lovastatin production by supplementing polyketide antibiotics to the submerged culture of Aspergillus terreus. Appl Biochem Biotechnol, 160(7), 2014-25. doi:10.1007/s12010-009-8762-1
[9] Kim, H., Moon, J.Y., Kim, H., Lee, D.S., Cho, M., Choi, H.K., Kim, Y.S., Mosaddik, A., Cho, S.K. (2010) Antioxidant and antiproliferative activities of mango (Mangifera Indica L.) flesh and peel. Food Chemistry, 121, 429-436. doi:10.1016/j.foodchem.2009.12.060
[10] Pandey, A. (2003) Solid-state Fermentation. Biochemical Engineering Journal, 13, 81-84. doi:10.1016/S1369-703X(02)00121-3
[11] Wei, P., Xu, Z., Cen, P. (2007) Lovastatin production by aspergillus terreus in solid state fermentation. Journal of Zhejiang, 8(9), 1521-1526. doi:10.1631/jzus.2007.A1521
[12] Vilches Ferron, M.A., Casas Lopez, J.L., Sanchez Perez, J.A., Fernandez Sevilla, J.M., Chisti, Y. (2005) Rapid screening of aspergillus terreus mutants for overproduction of lovastatin. World journal of microbiology & biotechnology, 21, 123-125. doi:10.1007/s11274-004-3045-z
[13] Valera, H.R., Gomes, J., Lakshmi, S., Gururaja, R., Suryanarayan, S., Kumar, D. (2005) Lovastatin production by solid state fermentation using aspergillus flavipes. Enzyme and microbial technology, 37, 521-526. doi:10.1016/j.enzmictec.2005.03.009
[14] Chen, F., and Hu, X. (2005) Study on red fermented rice with high concentration of monacolin K and low concentration of citrinin. International Journal of food Microbiology,103(3),331-337. doi:10.1016/j.ijfoodmicro.2005.03.002
[15] Pansuriya, R.C., and Singhal, R.S. (2010) Response surface methodology for optimisation of production of Lovastatin by Solid State Fermentation. Brazilian Journal of Microbiology, 41, 164-172. doi:10.1590/S1517-83822010000100024
[16] Sayyad, S.A., Panda, B.P., Javed, S., Ali, M. (2007) Optimization of nutrient parameters for lovastatin production by Monascus purpureus MTCC 369 under submerged fermentation using response surface methodology. Applied. Microbio. Biotech,73, 1054-1058. doi:10.1007/s00253-006-0577-1

  
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