Investigation of an inflammatory viral disease HBV in cardiac patients through polymerase chain reaction

Farina Saher, Khalil ur Rehman, Javed A. Qureshi, Muhammad Irshad, Hafiz M. Nasir Iqbal

Department of Chemistry and Bi?Chemistry, University of Agriculture, Faisalabad, Pakistan.
Department of Microbiology, University of Agriculture, Faisalabad, Pakistan.
Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE) Faisalabad, Pakistan.

DOI: 10.4236/abb.2012.324059                

Abstract

In the present study 100 cardiac patients were randomly selected from the cardiology ward, Allied Hospital Faisalabad, Pakistan. All the selected cases were analysed for different parameters like Hepatitis B surface Antigen (HbsAg), Bilirubin, Alkaline phosphatase, serum glutamic pyruvic transaminase, and serum glutamic-oxaloacetic transaminase. Out of total 16% patients were lying in the age of 21-30 year, 25% in the age of 31-40 year, 35% in the age of 41-50 year, 19% in the age of 51-60 years and 5% patients in the age of 61-70 years. No subject was found positive in age group 21-30 years patients. 35% patients have higher value of SGPT while, other 26% were with higher value of SGOT. Rest of the 32 and 24% have higher ALP and Bilirubin levels, respectively. Assay profile revealed that ALP level was increased with increasing age, body mass index, Creactive protein, diabetes, smoking, sex, serum uric acid, lead, cadmium, hypercholesterolemia, lesion of liver and cardiovascular disease. The serum of the eight HbsAg positive cases were tested for the presence of HBV through PCR and no sample was found positive. At the end of the study, PCR amplified samples were run on 1.5% agarose gel to confirm the case.

Keywords

HBV; Liver Inflammation; LFTs; PCR; Agarose Gel Electrophoresis

Conflicts of Interest

The authors declare no conflicts of interest.

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